Biological effects of high voltage electrostatic field on gametophytes of sea mustard Undaria pinnatifida
LI Xiao-li1, LU Ying1, HU Yu-cai2, SUN Pi-hai3, XING Dong-fei1
1.Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China; 2.College of Sciences, Dalian Ocean University, Dalian 116023, China; 3.Science and Technology Park Company Limited，Dalian 116023, China
Abstract: The gametophytes of selective referred sea mustard Undariapinnatifida strain DL18 were treated with high voltage electrostatic field at electric field intensity of 0, 3.3, 4.0, 4.7, 5.3, and 6.0 kV/cm for 2-10 min and then reared in an indoor and cultivated on the sea to investigate their growth and development. The rbcL, rbcL-rbcS spacer, and partial of rbcS sequences(rbcL-sp-S) was analyzed in F1 generation. It was found that survival rate of the gametophytes was all above 95% within electric field intensity of 3.3-6.0 kV/cm for 2-10 min, though survival rate of the gametophytes was shown to be gradually reduced with the increase in electric field intensity and duration, without significant difference in maturation rate, fertilized rate of mature eggs and germination rate of fertilized eggs of male and female gametophytes between the treatment groups and control group (P>0.05). However, there were significant differences in specific growth rate (SGR) of gametophytes and length of juvenile sporophytes between the treatment groups and control group (P<0.05), the maximal average length (2.4-2.8) m and weight(1.6-2.0) kg of F1 generation sporophytes in treatment groups and the maximal average length (2.2±0.3) m and weight (1.2±0.2) kg in control group in April at electric field intensity of 5.3 kV/cm. The best growth was observed in treated gametophytes and their F1 generation juvenile and adult sporophytes at electric field intensity of 5.3 kV/cm, with the maximal length of (2.8±0.5)m and weight of (2.0±0.3)kg. The rbcL-sp-S sequence analysis revealed that these fragments were all above 2100 bp in size, with average A+T content of 64.22% in the treatment groups and the control group, higher A+T content than G+C content. There was more base replacement and base deletion and insertion in the treatment groups than that in the control group, and there was greater genetic distance of F1 generation gametophytes between the treatment groups and the control group, increased from 0.002 4 to 0.005 7 with the increase in the electric field intensity, with genetic distance of 0.003 3 between the F1 generation gametophytes and the F2 generation gametophytes in the control group. The finding provides theoretical guidance for the application of high voltage electrostatic field technology in improvement of germplasm and breeding of sea mustard.
李晓丽, 芦莹, 胡玉才, 孙丕海, 邢冬飞. 高压静电场激励裙带菜配子体的生物效应研究[J]. 大连海洋大学学报, 2018, 33(4): 413-422.
LI Xiao-li, LU Ying, HU Yu-cai, SUN Pi-hai, XING Dong-fei. Biological effects of high voltage electrostatic field on gametophytes of sea mustard Undaria pinnatifida. Journal of Dalian Ocean University, 2018, 33(4): 413-422.
2018, Vol. 33 
