Sequence and SNP analysis of Kelch-like gene from pearl oyster Pinctada fucata martensii
1.College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;2.Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province,Zhanjiang 524088,China;3.Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding,Zhanjiang 524088,China；4.Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang),Zhanjiang 524006,China
Abstract: In order to explore the function of Kelch-like gene in pearl oyster Pinctadafucatamartensii,the full-length sequence and SNPs of Kelch-like were analyzed in the present study.It was found that the full-length sequence of Kelch-like was 2 127 bp containing a 5′UTR of 58 bp and a 3′UTR of 44 bp.The open reading frame had length of 2 025 bp encoding 674 amino acids.The Kelch-like sequence contained a BTB domain,a BACK domain and two Kelch domains.The pearl oyster Kelch-like had high homology with Kelch-like of other species in amino acid sequence.Multi-sequence alignment indicated that the BTB and BACK domains were highly conservative,and Kelch domain held a conservative GG site.Kelch-like gene showed significantly higher expression in the marginal zone of mantle (P<0.05),especially high expression (up-regulated) level in the 6,12 h and 120 h in the repair process of the pearl oyster with damaged mantles (P<0.05).SNPs analysis of Kelch-like exon region revealed that 31 SNP sites in 56 SNP obtained were moderately polymorphic and the rest were low-level polymorphisms.Among the 56 SNPs,10 SNP loci formed a haplotype set containing,4 haplotypes,of which the haplotype AATCTGTCGG was an excellent haplotype,with significantly greater shell weight than the haplotype AGCCTACCGA and haplotype AGCCTACTTA (P<0.05).These findings showed that Kelch-like may participated in the shell prismatic layer and haplotype AATCTGTCGG hold the potential as the molecular maker of shell weight in the breeding process,which are of great significance to the breeding of pearl oyster with excellent economic traits.
2022, Vol. 37 
