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LI Zhijun
LIU Ying
ZHANG Dongdong (executive)
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10 April 2025, Volume 40 Issue 1
  
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  • Research progress in the aquaculture biology of the Golden Pompano (Trachinotus ovatus)
    CHEN Huapu
    2025, 40(1): 1-11. https://doi.org/10.16535/j.cnki.dlhyxb.2024-155
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    The golden pompano(Trachinotus ovatus) is a popular fish variety for marine cage culture and large-scale offshore aquaculture facilities.Successive breakthroughs in key technologies for breeding and cultivating golden pompano have promoted the rapid development of its industrial aquaculture in China.However,issues such as the decline in farming performance,caused by relatively weak fundamental biological and genetic breeding research,have become bottlenecks for sustainable development of this industry.We describe the current state of research and development in the industry and review the biological characteristics of T. ovatus,including resource distribution,habitat,morphological traits,and reproductive biology.We also summarize recent research achievements in artificial breeding,aquaculture,physiological ecology,and genetics.In light of the development,conservation,and the future potential of golden pompano resource,we propose future research directions,including selective breeding,basic genetic mechanisms,gonadal development regulation,and new strain development,to provide references for the future stable development of golden pompano aquaculture sector.
  • Effects of cold exposure on the immune indicators and gene expression of Ruditapes philippinarum
    CHEN Sunan, KONG Xue, SONG Manzong, CAI Yuefeng, ZHI Ying, SHEN Xin
    2025, 40(1): 12-24. https://doi.org/10.16535/j.cnki.dlhyxb.2024-121
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    The Manila clam,Ruditapes philippinarum,is an economically important bivalve shellfish whose survival is affected by a variety of environmental factors,temperature being a key factor affecting its normal activities.Much attention has been given to the response of shellfish to high temperature,while fewer studies have been conducted on their response to low temperature.Considering the increasing occurrence of extreme weather,such as extreme cold,research is required on the adaptation of shellfish to low temperatures.In this study,R. philippinarum was exposed to low temperature for 60 days.Its immune indicators were systematically measured and analyzed,and the differentially expressed genes (DEGs) obtained were analyzed by using the GO/KEGG enrichment databases.A total of 8 344 and 2 674 DEGs were found in the gill tissues of R. philippinarum after 20 and 60 days of cold exposure,respectively.Enrichment analysis of these DEGs revealed that R. philippinarum has potential low temperature coping strategies in biological processes such as protein processing,substance metabolism,antioxidant,immune and anti-apoptosis.Regarding protein processing,the expression of the SAR1B,HSP20,HSP70,TRX,and SEC61G genes were up-regulated during protein processing,suggesting that R. philippinarum may maintain cellular stability at low temperatures by enhancing protein synthesis.Phosphatidylcholine (PC) is a key component of cell membranes,and the up-regulated expression of PC synthesis-related genes in this study suggests that R. philippinarum may maintain cell membrane stability under cold exposure by enhancing PC synthesis.In this study,the expression of the SOD,CYP450,GST,and GPX genes were up-regulated,and the MDA content decreased when the SOD activity increased during the late stage of cold exposure,suggesting that hypothermia induces SOD to scavenge excessive ROS in the organism,thus protecting it from hypothermic injury.In addition,the immune and anti-apoptosis related genes were also significantly activated at low temperature.These results provide basic data for furtherstudy on the mechanism of low-temperature tolerance in R. philippinarum and provide theoretical support for the selection and breeding of low-temperature tolerant shellfish varieties.
  • Structure and expression characteristics of taurine transporter CgTauT genes in Crassostrea gigas
    JIANG Chunyu, KONG Ning, SUN Shiqing, LI Xiang, LUO Cong, QIAO Xin, WANG Lingling, SONG Linsheng
    2025, 40(1): 25-33. https://doi.org/10.16535/j.cnki.dlhyxb.2024-125
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    To investigate the composition and expression characteristics of the taurine transporter (CgTauT) gene family in Crassostrea gigas,a genome-wide screening strategy was used to identify taurine transporter genes,and the qRT-PCR was used to analyse their tissue distribution pattern and to expression characteristics after taurine treatment.The results revealed that C. gigas contains four CgTauT genes,with each containing an SNF structural domain and 12 transmembrane domains.The four CgTauT genes were constitutively expressed in the various tissues of C. gigas,with CgTauT1 showing the highest expression in the adductor muscle,and CgTauT2,CgTauT3 and CgTauT4 showing the highest expression in the mantle.There were no significant correlations between the expression levels of the four CgTauT genes and the taurine contents of the tissues.Under taurine immersion treatment,the four CgTauT genes exhibited different temporal expression profiles in the mantle of C. gigas.The expression level of CgTauT1 increased after three hours of treatment.With prolonged taurine treatment,the mRNA expression level of CgTauT3 increased gradually,while that of CgTauT4 first increased and then decreased.These findings provide a theoretical foundation for further exploration of the physiological functions of CgTauTs and the molecular mechanisms underlying taurine accumulation in C. gigas.
  • Identification of the Duox gene in Exopalaemon carinicauda and preliminary study on its immune function
    LIU Dexue, FAN Menghao, CHENG Weitao, CAO Yanrong, HAN Wanyu, HU Guangwei
    2025, 40(1): 34-43. https://doi.org/10.16535/j.cnki.dlhyxb.2024-157
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    Dual oxidase (Duox) is a member of the nicotinamide adenine dinucleotide (NADPH) oxidase (NOX) family,which plays a crucial role in various biological processes,such as the production of reactive oxygen species,regulation of the biological clock,and the immune response.To explore the immune function of the Duox gene in Exopalaemon carinicauda,the cDNA sequence of the Duox gene was successfully cloned from E. carinicauda,and the amino acid sequence which it encoded was analyzed.Real-time fluorescence quantitative PCR technology (qRT-PCR) was used to analyze the tissue expression characteristics of gene and its expression changes infected with Vibrio parahaemolyticus.Finally,the mortality rate of E. carinicauda infected with V. parahaemolyticus after Duox gene knockdown was analyzed by using the RNA interference technique.The result shows that the coding region sequence length of the Duox gene in E. carinicauda is 4 518 bp,encoding 1 505 amino acids,reflecting typical structural characteristics of the dual oxidase.The Duox gene is expressed in all of the E. carinicauda tissues tested,with the highest expression level in gill tissues.After infected with V. parahaemolyticus,the Duox gene expression level in gills increases significantly (P<0.05).Infection with V. parahaemolyticus after injection of siRNA reagent significantly reduces the survival rate of E. carinicauda in the RNA interference group compared with the control group (P<0.05).This study shows that the Duox gene plays a role in the immune response of E. carinicauda against pathogenic infection,and provides an favorable reference for further exploration into the immune function and mechanism of Duox in E. carinicauda.
  • An experimental study of an in situ labeling technique for Mactra veneriformis
    GAO Jin, XIE Xi, BAI Yongan, PANG Jinchi, LIU Xiangfeng, TENG Weiming, WANG Qingzhi
    2025, 40(1): 44-54. https://doi.org/10.16535/j.cnki.dlhyxb.2024-096
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    The use of calcein as an in situ labeling tool for Mactra veneriformis was examined.The optimal labeling concentration and immersion time were explored by using indicators such as the individual survival rate,the success rate of fluorescence labeling,the rate of wel-labeled individuals,and the antioxidant enzyme activity in the digestive glands and lipid markers in the soft tissues.An one-month in situ labeling experiment was conducted in Geligang in the Liaodong Bay,based on the most suitable labeling conditions.The result shows that two labeling schemes,0,25,50,75 mg/L calcein immersion for 1.5 hours and 0.50 mg/L calcein immersion for 0.5 and 1.5 hours,had no significant negative impacts on M. veneriformis.Except for the well-labeled rate of 54.90%±8.99% for the 25 mg/L calcein immersion for 1.5 hours group,the success rate and well-labeled rate of all other treatment groups were 100%.This indicates that calcein has the ability to mass-label M. veneriformis in a short period of time.The treatment involving immersion in 50-75 mg/L calcein for 0.5 to 2 hours could serve as a method for in situ labeling of M. veneriformis in intertidal flats.The results of the in situ labeling experiment indicate that immersion in 75 mg/L calcein for 2 hours produces the best results,with an average recapture rate of 10.00%±3.33% after one month,a success rate of 36.11%±12.73%,and a well-labeled rate of 100%.These results provide scientific references for the development of in situ labeling techniques for intertidal shellfish and the conservation of native high-quality clam resources.
  • Study on the expression pattern of Cathepsin B in Yesso scallops (Patinopecten yessoensis) infected with Polydora
    HANG Yunna, SUN Hongyan, TANG Anqi, MAO Junxia, TIAN Ying, WANG Xubo, HAO Zhenlin, DING Jun, CHANG Yaqing
    2025, 40(1): 55-64. https://doi.org/10.16535/j.cnki.dlhyxb.2024-110
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    Cathepsin B (CatB) is a lysosomal cysteine protease with important immune functions.To investigate the molecular function of CatB in Yesso scallops (Patinopecten yessoensis) infected by Polydora,genomic identification,sequence characterization,and gene expression analyses of the CatB gene were conducted in P. yessoensis (PyCatB),and the changes of PyCatB enzyme activity and gene expression pettern were analyzed.The results showed that the full length of the PyCatB gene was 9 267 bp with nine exons and eight introns.The cDNA length of PyCatB was 1 219 bp,with a 5′UTR of 103 bp and a 3′UTR of 99 bp,and an open reading frame length of 1 017 bp,encoding 338 amino acids.PyCatB was predicted to contain three conserved functional domains; the N-terminal signal peptide; Propeptide C1; and peptidase C1A cathepsin B.Additionally,it also had a catalytic triplet structure (Cys116,His284,Asn304),a Gln110 oxygen anion pore,a GCNGG motif,and a characteristic occluding loop formed by C194/C205 fixed residue fragments,which played important roles in the catalytic activity of PyCatB.Phylogenetic analysis showed that the evolutionary status of CatB was consistent with the species taxonomy.Enzyme activity analysis showed that the enzyme activity of CatB in the mantle tissue of P. yessoensis infected with Polydora was significantly higher than that in healthy scallops.Gene expression analysis showed that PyCatB was expressed at different levels in the various tissues of healthy adult scallops except for the adductor muscle,indicating that PyCatB was involved in a wide range of physiological processes.The expression levels of PyCatB in different mantle regions of infected scallops were all significantly higher than those in healthy scallops,but there was no significant difference between the hemocytes of healthy and infected scallops.It was speculated that PyCatB probably acted mainly through the mantle tissue to participate in the immune response processes of P. yessoensis to resist Polydora infection.This study provides a reference for analyses of the mechanism whereby P. yessoensis resists Polydora infection and lays an important theoretical foundation for Polydora infection control in molluscs.
  • Glucose metabolism and relevant response of Gymnocypris przewalskii exposed to chronic alkalinity stress
    GAO Zihan, WEI Wei, YUE Miao, LI Yixin, CUI Yanrong, XU Baoke, LI jin, WEI Fulei, LIANG Jian
    2025, 40(1): 65-75. https://doi.org/10.16535/j.cnki.dlhyxb.2024-042
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    To inquire mechanism of Gymnocypris przewalskii adaptation to alkalinity changes through glucose metabolism,G. przewalskii with body weights of 126.67 g±8.88 g were reared in an 85 cm×56 cm×62 cm tank containing either freshwater (control group) or four experimental alkalinities of 25%,50%,75% and 100%,prepared using NaHCO3 and Na2CO3 dissolved in tap water (NaHCO3∶Na2CO3 = 9∶1).The important indicators of G. przewalskii glucose metabolism were determined,including glycogen content,glucose (GLU) content,and the activities of pyruvate (PA),hexose kinase (HK),pyruvate kinase (PK),phosphoenolpyruvate (PK),phosphoenolpyruvate carboxylase (PEPCK),glucose 6 phosphate dehydrogenase (G6PD),and fructose 1,6 bisphosphate aldolase (FBA).The results showed that,with increasing alkalinity,the hepatic glycogen,muscle glycogen,and GLU content in the gills and kidneys increased and then decreased,and the GLU in the muscle gradually decreased.As the alkalinity increased,PA in the gills increased and then decreased,and PA in the kidneys,liver,and muscle decreased and then increased.The activities of HK,PK,PEPCK and G6PD in the gills showed an increasing trend with increasing alkalinity,and the activities of FBA in the kidneys and liver gradually rose.A heatmap showed that glucose metabolism processes in the gills,kidneys,and liver were promoted by increasing alkalinity to some extent,while glucose metabolism in the muscle was inhibited as alkalinity increased.As alkalinity increased,fba-c was commonly expressed in the gills,kidneys,intestine,and liver,and the expression level of fba-c in the gills and kidneys increased and then decreased.The maximum expression levels were observed in the 75% alkalinity group.In conclusion,the gills,kidneys,and liver responded to alkalinity changes by increasing glucose metabolism,while muscle responded to alkalinity changes by inhibiting glucose metabolism,leading to rapid adaptation to different alkalinity levels in G. przewalskii.
  • Immunomodulatory effects of enzymolytic products of Perinereis aibuhitensis in immunosuppressed mice
    LI Lijuan, ZHU Guoping, CAO Wenhong, CHEN Zhongqin, TAN Mingtang
    2025, 40(1): 76-85. https://doi.org/10.16535/j.cnki.dlhyxb.2024-088
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    The enzymolytic products of Perinereis aibuhitesis were used to explore the immunomodulatory effects of P.aibuhitensis in immunosuppressed mice.The immunosuppressed mouse model was established by using cyclophosphamide induction,and the changes in body mass indexes,immune organ indexes,splenic T lymphocyte subset,total white blood cells,and immunoglobulin concentration of mice in the untreated group,model group,and sample group (the enzymolysis products below<5 000,5 to 8 000,>8 000 in P. aibuhitensis) were measured.The results showed that the three ultrafiltration components of enzymolytic products from P. aibuhitensis could increase the immune organ indexes of mice,the number of CD3e+ on T cells in the spleen,and the concentration of immunoglobulin,all of which have particular immune functions.The high dose of enzymolytic products below<5 000 in P. aibuhitensis showed the highest efficacy and significantly increased the body mass indexes,immune organ indexes,and total white blood cells (P<0.05) of the mice.And the number of CD3e+ and the ratio of CD4+/CD8a+ were significantly increased (P<0.01) and the effect of plasma cells on immunoglobulin secretion was enhanced.Perinereis aibuhitensis can ameliorate the immune impairment induced by immunosuppressants and enhance the immunomodulatory effect in immunosuppressed mice and therefore has the potential for development as an immunomodulator.
  • Screening and identification of a highly efficient denitrifying functional bacterium XYB4 and its effects on the growth and intestinal flora of Litopenaeus vannamei and water quality
    WU Jiaqi, ZHOU Jianing, XUE Yuan, WANG Furui, ZHU Miao, SUN Chengbo
    2025, 40(1): 86-94. https://doi.org/10.16535/j.cnki.dlhyxb.2024-126
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    To develop highly efficient and economic denitrifying technology for an aquaculutre system,the experiment isolated a highly efficient denitrifying bacterium from a biofloc in shrimp culture pond ( named as XYB4) by morphological screening,physiological and biochemical identification,molecular biological assay,safety evaluation test,and denitrifying effect under the influence of different physical and chemical factors.The result shows that the XYB4 strain is a gram-positive bacillus.16S rDNA sequence result shows that it is Bacillus subtilis.The safety evaluation test shows that XYB4 is not lethal to Litopenaeus vannamei,without hemolysis,and is sensitive to 14 types of antibiotics.The best conditions for denitrification by the XYB4 strain are as follows:salinity 30,temperature 30 ℃,pH 7.5,and C/N 10-15.A mixture of XYB4 with feed (B7),splashed into an aquaculture pond (P7),significantly reduced the concentrations of  and  in the cultured water (P<0.05),and significantly increased the growth rate and specific growth rate of L. vannamei by 16.32%,17.52%,and 40.18%.and 40.06%,respectively.There was no significant difference in survival rate among the three groups (P>0.05).The richness of the L. vannamei intestinal flora and the water flora is significantly increased in the experimental group (P<0.05).The addition of the XYB4 strain inhibited the growth of harmful bacteria,such as Photobacterium and Tenacibaculum,and promoted the growth of beneficial nitrifying bacteria,such as Nitrospirae.In conclusion,the application of XYB4 can effectively reduce the concentrations of  and  in aquaculture water,promote the growth of L. vannamei,improve the richness of the microflora communities in the water and L. vannamei intestines,and inhibit the growth of some harmful microbial populations.The XYB4 has a high application value in aquaculture production.
  • Proteomics analysis of the Yesso scallop (Patinopecten yessoensis) with different shell colors based on quantitative tandem mass tag labeling technology
    TANG Anqi, YUAN Changzi, HANG Yunna, MAO Junxia, WANG Xubo, TIAN Ying, HAO Zhenlin, DING Jun, CHANG Yaqing
    2025, 40(1): 95-106. https://doi.org/10.16535/j.cnki.dlhyxb.2024-095
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    To explore the molecular mechanism causing shell color formation in the Yesso scallop (Patinopecten yessoensis),a proteomics analysis was performed on the mantle tissue of P.yessoensis with two different shell colors (brown and white) by using quantitative tandem mass tag (TMT) labeling technology.A total of 5 177 credible proteins were identified in the mantle tissue,of which 343 differentialy expressed proteins (DEPs) were detected between the two shell color types,with 187 up-regulated and 156 down-regulated DEPs in the white individuals.GO and KEGG functional enrichment analysis showed that melanin biosynthesis process,calcium ion regulation,and neural regulation related functions and molecular pathways were significantly enriched in the DEPs,suggesting that they play an important role in the formation and regulation of shell color in P.yessoensis.Immune-related functions and molecular pathways were also significantly enriched,indicating that there may be immunity differences between the two shell color types.A protein-protein interaction network was constructed and interaction relationships among the DEPs were predicted.Finally,the expression levels of DEPs were validated by using qRT-PCR technology,proving the reliability of the experiment.This study provides more evidence for the mechanism underlying shell color formation in P.yessoensis,and establishes a theoretical grounds for the breeding of disease resistant mollusk varieties with shell color as the objective trait.
  • Effects of different temperatures on the growth of Fabrea salina
    ZHANG Xinyue, CUI Wenting, PAN Wei, SUN Sihan, LIU Jiale, QI Hongli
    2025, 40(1): 107-116. https://doi.org/10.16535/j.cnki.dlhyxb.2024-098
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    To explore the effect of temperature on the population dynamics of Fabrea salina,the Montagnes population model was used to study the population dynamics of F. salina feeding different diets,such as Dunaliella salina,Isochrysisgalbana galbana,Chlorella vulgaris and Chaetocerosmuelleri under the different temperatures of 15 ℃,25 ℃,35 ℃.The result shows that the Montagnes model is suitable for evaluating the effects of the different temperatures on population dynamics of F. salina.The maximum feeding rate (Imax) of F. salina generally increases with increasing temperature.The peak (Imax) of F. salina feeding on C. vulgaris is 5.832×104 ind./h at 35 ℃.For D. salina,the (Imax) drops to a minimum of 9.150×102 ind./h at 15 ℃.The maximum birth rate (Bmax) of F. salina under the four diets generally increases with increasing temperature.The maximum Bmax of F. salina feeding on I. galbana is 1.764 1/d at 35 ℃,while the maximum (Bmax) of F. salina fed C. vulgaris is negative at 15 ℃.The analysis indicates that 15 ℃ limits the growth and feeding activities of F. salina,while 35 ℃ promotes its feeding and population growth rate.Its highest conversion efficiency and highest minimum mortality (Dmin) were observed at 35 ℃,as was the saturated feed concentration of the prey D. salina.At 25 ℃,the conversion efficiency (e) of D. salina diet was relatively high compared with the other three diets,and the minimum mortality rate (Dmin) was the smallest,and can be used as the criteria for large-scale,high-density cultures of F. salina.Fabrea salina has a relatively high temperature tolerance range and could be used as an excellent food organism.
  • Effect of different feeds on intestinal tissue and structural characteristics of bacterial community in the sea cucumber (Apostichopus japonicus)
    PEI Honglin, WANG Luo, DING Jun, CHENG Chao, CHANG Yaqing
    2025, 40(1): 117-125. https://doi.org/10.16535/j.cnki.dlhyxb.2024-132
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    The study investigated the effects of different feeds on the intestinal tissue and bacterial community of the sea cucumber (Apostichopus japonicus).Sea cucumbers were cultured in net cages in the offshore waters of Xiapu,Fujian Province and fed either a seaweed diet (HD group) or a granulated feed (SL group) for 90 days.Analysis of the intestinal morphological features showed that the average length of the mucosal layer in the HD and SL groups was (194.4±21.0 ) μm and (204.9±17.1 ) μm,respectively,that the average thickness of the muscle layer was (5.2±1.6 ) μm and (6.3±1.9 ) μm,respectively,and that,compared with the HD group,the length of the intestinal mucosal layer and thickness of the muscle layer in the SL group were increased by 5.4% and 21.2%,respectively.High-throughput sequencing results showed that the abundance and diversity of the sea cucumber gut microbiotas were not significantly affected by the different diet types (P>0.05),but that the diet types did influence the composition of the sea cucumber gut microbiotas.Under different food sources,the predominant bacterial phylum,Proteobacteria,showed relative abundance ranges from 34.28% to 47.75%.The subdominant phyla showed significant changes.The most common in both the HD and SL groups were Bacteroidetes and Firmicutes,with relative abundances of 23.22% and 31.80%,respectively.At the genus level,the predominant bacteria in the HD and SL groups were Formosa and Bacillus spp.,with relative abundances of 15.92% and 13.40%,respectively.The study indicates that granulated feed can promote the colonization of beneficial intestinal bacteria,stabilize the structure of the gut microbiota,and significantly reduces the relative abundance of potentially opportunistic pathogens (P<0.05) and provides a theoretical basis for improvements in the management of sea cucumbers in aquaculture cages.
  • Effect of dietary methionine level on growth,biochemical indicators,digestive enzyme activity and antioxidant capacity of larval and juvenile hybrid snakehead (Channa maculate♀×Channa argus♂)
    QIN Yao, HU Bo, XIONG Pan, LIANG Haohui, ZOU Jixing, ZHU Xifeng, WANG Guoxia
    2025, 40(1): 126-134. https://doi.org/10.16535/j.cnki.dlhyxb.2024-152
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    The experiment investigated the effects of dietary methionine level on growth performance,intestinal digestion,and antioxidant capacity of larval and juvenile hybrid snakehead (Channa maculate♀×Channa argus♂).2 400 larval and juvenile hybrid snakeheads with an initial body weight of 100 mg were selected and randomly divided into six groups with four replicates and 100 repeats each.Each replicate was placed into a nylon mesh boxwith a water volume of 50 L in an outdoor breeding system and fed with isonitrogen and isolipid diets,supplemented with either 0%,0.5%,1.0%,1.5%,2.0% or 2.5% of methionine (measured values of 0.67%,1.08%,1.43%,1.78%,2.22% and 2.61%,respectively).The trial period was 30 dys.The results showed that:the weight gain rate (WGR),specific growth rate (SGR) and protein efficiency ratio (PER) of the test fish first increased and then decreased with increasing dietary methionine level; and that the Feed Conversion Ratio (FCR) first decreased and then increased.The WGR and SGR in the 1.08%,1.43% and 1.78% groups were significantly higher than in the 0.67% group (P<0.05); the FCR was significantly lower in the 1.08%,1.43% and 1.78% groups than in the 0.67% group (P<0.05); the viscerosomatic index (VSI) in the 1.78% group was significantly lower than in the other test groups (P<0.05); the liver triglyceride (TG) in the 0.67% group was significantly greater than in the other test groups (P<0.05); the Total Cholesterol (TC) was significantly higher in the 1.08% group than in the 2.22% and 2.61% groups (P<0.05); the Aspartate transaminase (AST) in the 1.78% group was significantly higher than in the other test groups (P<0.05); both trypsin and lipase activities in the intestine were significantly higher in the 1.78% group than in the 0.67% test group; the liver T-SOD was significantly higher in the 1.78% group than in the 2.22% group; GSH-Px was significantly higher in the 1.34% group than in the 1.42%,1.78%,and 2.61% groups; and MDA was significantly lower in the 2.61% group than in the other groups (P<0.05).The addition of suitable amounts of methionine to the feed can therefore improve the growth performance of both larval and juvenile hybrid snakehead,reducing the feed coefficient,and improving the digestive enzyme vitality and antioxidant capacity.Taking the SGR as the evaluation index,a broken-line regression analysis showed that the optimal level of methionine supplementation was 1.72%,accounting for 3.22% of the feed protein.
  • Structural characteristics of microbial community in the water and sediments of recirculating aquaculture systems for Apostichopus japonicus
    LIU Dan, PAN Haoran, NI Hongdong, ZHAO Xinyan, XU Tianhao, WANG Nanyi, DING Jun, CHANG Yaqing, TIAN Yi
    2025, 40(1): 135-145. https://doi.org/10.16535/j.cnki.dlhyxb.2024-133
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    The taxonomic composition of the microbial flora in the water and sediments of recirculating aquaculture systems for Apostichopus japonicus were studied by using 16S rRNA high-throughput sequencing in different months of year.The Alpha diversity results showed that the diversity and richness of microbial flora in the recirculating water of aquaculture tank were significantly higher than those in the sediments (P<0.05).Comparing the differences of the microbial community composition between the water and sediments,the relative abundance of Bacteroidetes in the sediments was found to be significantly higher than in the water (P<0.001),and the relative abundance of Actinomycetes in the sediments was significantly lower than in the water (P<0.05).The relative abundances of Lutibacter and Pseudoruegeria in the sediments were significantly higher than in the water (P<0.05).Regarding the monthly differences at the phylum level in the water:the proportion of Cyanobacteria was higher in May; the proportions of unidentified Bacteria and Chloroflexi were higher in July; the proportions of Octadecabacter and unidentified Cyanobacteria were higher in May; the proportion of Arcobacter was higher in July; and the proportions of Marinobacterium and Glaciecola were higher in September.Regarding the monthly differences at the phylum level in the sediments:Cyanobacteria were higher in September; Glaciecola,unidentified_Flavobacteriaceae and Octadecabacter were higher in May; Arcobacter and Agarivorans were higher in July; and unidentified Cyanobacteria were higher in September.The results showed that the dominant taxa in the water and sediments were different in different months of the year,and could provide a reference for the rational utilization of anti-microbial agents in the healthy culture of A. japonicus in recirculating water aquaculture systems.
  • Comparative analysis between germplasm characteristics of cultured stocks of Parabramis pekinensis in the Yangtze River and in the Heilongjiang River Basin
    SHI Changhe, ZHENG Xianhu, SUN Zhipeng, LIU Tianqi, NA Rongbin, LU Cuiyun
    2025, 40(1): 146-155. https://doi.org/10.16535/j.cnki.dlhyxb.2024-129
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    To explore the difference in germplasm characteristics of Parabramis pekinensis in Yangtze River from that in Heilongjiang River,and improve the germplasm resources of this species in the Heilongjiang River,the external morphological characteristics,muscle nutrient compositions,and genetic structures of cultured stocks of P. pekinensis in the Yangtze River (WB) and the Heilongjiang River (FB) were analyzed.An analysis of the morphological data shows significant differences (P<0.05) between the WB and FB populations in the ratios of total length (TL),body height (BH),head length (HL),snout length (WL),eye diameter (ED),eye cross (EC),and caudal peduncle height (FH) compared with the standard length (SL).The body shape of fish species from Changjiang River is considerable different to that from Heilongjiang River and fish stocks from Changjiang River may be used as the hybrid breeding stocks of P. pekinensis.Muscle nutrient composition analysis shows that the crude lipid content (0.63 g/100 g) of the WB stock is significantly lower than that of the FB stock (1.57 g/100 g) (P<0.05),and that the difference in crude protein contents is not significant (P>0.05).The total amino acid,semi-essential amino acid,non-essential amino acid,and flavor-amino acid contents of the WB stock are significantly higher than those of the FB stock (P<0.05).But the saturated fatty acid,monounsaturated fatty acid,and polyunsaturated fatty acid contents of the WB stock are significantly lower than those of the FB stock (P<0.05).Analysis of the genetic structure based on genome wide SNP markers reveals that the polymorphic information contents (PIC) of the WB and FB stocks are 0.100 7 and 0.100 4,respectively,indicating low levels of genetic diversity (PIC<0.25).The genetic differentiation coefficient (Fst) between the stocks is 0.030 5,and the gene flow (Nm) iss 7.942 8,suggesting extensive gene exchange between the two stocks.This study provides a favorable reference for the development,breeding,and sustainable utilization of P. pekinensis.
  • Stock assessment of Sciaenidae fishes in the coastal waters of Zhejiang Province
    JIN Zihui, XU Kaida, ZHANG Hongliang, ZHOU Yongdong, LONG Xiangyu, ZHAN Wei, MA Wenjing, XU Fei
    2025, 40(1): 156-165. https://doi.org/10.16535/j.cnki.dlhyxb.2024-137
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    The coastal waters of Zhejiang Province are important breeding grounds for major economic fishes,including species of the Sciaenidae family with high nutritional value,represented by large yellow croaker (Larimichthys crocea) and small yellow croaker (Larimichthys polyactis).To assess the resource status of Sciaenidae off the coast of Zhejiang,the bottom trawling survey data were used from the coastal area of Zhejiang (27°00′-31°00′ N,120°30′-123°45′ E) collected each spring (April) and autumn (November) from 2019 to 2021.The study used the swept sea area method and analyzed the seasonal and spatial distribution of main economic fishes of the Sciaenidae family,including their dominance,biomass density,numerical density,and existing biomass.The results showed that:the average biomass density of Sciaenidae off the coast of Zhejiang over the three years was 140.7 kg/km2,with autumn values consistently higher than those in spring and the highest values ascertained in the southern area; the biomasses of species ranking from the highest to lowest in survey years were brown croaker (Miichthys miiuy),silver croaker (Pennahia argentata) and small yellow croaker (Larimichthys polyactis); there was an overall gradual improvement in resource staus compared with historical data.The study provides a detailed description of the Sciaenidae fish resource densities and distribution status.The results provide a basis for future research and the development of conservation techniques and sustainable utilization management of offshore fishery resources in Zhejiang.
  • Stock assessment of Uroteuthis edulis in the East China Sea based on the JABBA
    WU Jiamin, LI Nan, FANG Zhou
    2025, 40(1): 166-173. https://doi.org/10.16535/j.cnki.dlhyxb.2024-140
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    To explore applicability of the just another bayesian biomass assessment (JABBA) in assessing the short-life species stocks such as Uroteuthis edulis,three surplus production models based in the JABBA were used to assess the U.edulis stock status in the East China Sea with catch and CPUE data from China,Taiwan Province of China,and Japan from 2009 to 2021.The results showed that the Schaefer model provided the best fit to the dataset.In 2021,the biomass of U.edulis in the East China Sea was 135.0×104 t,and the maximum sustainable yield (MSY) was 57.34×104 t.A retrospective analysis showed problems in the retrospective evaluation of stock assessment:the value of F/FMSY may be overestimated; and the stock biomass may be underestimated.A predictive analysis showed that when the total allowable catch (TAC) was set to 52.26×104 t (i.e.,the catch in 2021) or less (i.e.,the catch in 2021),the biomass of U.edulis showed an increasing trend,but was still lower than BMSY.In future,it will be necessary to consider the impact of environmental factors on U.edulis population dynamics,further adjust and optimize data collection and population modelling,consider the uncertainties in the parameter and model assumptions,and select a more appropriate stock assessment model.
  • Research progress and prospect of solid waste transport performance in recirculating aquaculture system:a review
    ZHAO Chenxu, WU Gang, SUN Wei, HU Wei, REN Xiaozhong, ZHANG Jun, WANG Xian
    2025, 40(1): 174. https://doi.org/10.16535/j.cnki.dlhyxb.2024-161
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    Recirculating aquaculture systems (RAS) are widely acknowledged as pivotal in developing future improved aquaculture practices because of their ability to rear a large number of aquatic organisms in a small space,conserve resources,and maintain environmental sustainability.Nevertheless,the buildup of solid waste in RAS presents a danger to the health of aquaculture species and has detrimental impacts on the functioning and productivity of a system.Efficient management and disposal of solid waste in RAS is crucial for maintaining optimum system operation.This study examines the categorization,origins,and potential consequences of solid waste in the RAS,and also explores methods to enhance waste transportation efficiency.Significant improvements in solid waste removal efficiency can be achieved by optimizing the design of aquaculture tanks,upgrading intake and drainage structures,modifying tank bottom gradients,utilizing diversion devices,and enhancing feed quality.These improvements will guarantee the consistent and effective functioning of a RAS.
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