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  • Applications and future prospects of DeepSeek and fishery intelligent agent in modern fisheries:a review
    MA Zhihong, LIU Ying
    Journal of Dalian Fisheries University. 2025, 40(2): 185-194. https://doi.org/10.16535/j.cnki.dlhyxb.2025-062
    Abstract (1089) PDF (1003) HTML (2)   Knowledge map   Save
    The rapid advancement of artificial intelligence (AI) has provided novel pathways for the intelligent transformation of the fisheries industry.This paper explores the application and development prospects of DeepSeek in fisheries.As a representative domestically developed open source large model,DeepSeek demonstrates high adaptability across multiple fisheries scenarios by leveraging its multimodal integration,dynamic reasoning,and knowledge graph construction capabilities.By constructing a DeepSeek-based fisheries intelligent agent,a new paradigm can be established for core production processes,including precision aquaculture management,breeding optimization,disease prevention,and resource stock assessment.Through interdisciplinary collaborative innovation,the co-construction of an “AI cooperative” ecosystem,technology scenario validation,and algorithm iteration,this study addresses critical challenges such as multisource data governance bottlenecks,industrial collaboration barriers,tacit knowledge conversion obstacles,and spatiotemporal decision making adaptation challenges.These efforts aim to achieve comprehensive integration of AI and fisheries practices,ultimately building a sustainable ecosystem for the intelligent evolution of the fisheries industry.
  • Identification of the Duox gene in Exopalaemon carinicauda and preliminary study on its immune function
    LIU Dexue, FAN Menghao, CHENG Weitao, CAO Yanrong, HAN Wanyu, HU Guangwei
    Journal of Dalian Fisheries University. 2025, 40(1): 34-43. https://doi.org/10.16535/j.cnki.dlhyxb.2024-157
    Abstract (1086) PDF (447) HTML (0)   Knowledge map   Save
    Dual oxidase (Duox) is a member of the nicotinamide adenine dinucleotide (NADPH) oxidase (NOX) family,which plays a crucial role in various biological processes,such as the production of reactive oxygen species,regulation of the biological clock,and the immune response.To explore the immune function of the Duox gene in Exopalaemon carinicauda,the cDNA sequence of the Duox gene was successfully cloned from E. carinicauda,and the amino acid sequence which it encoded was analyzed.Real-time fluorescence quantitative PCR technology (qRT-PCR) was used to analyze the tissue expression characteristics of gene and its expression changes infected with Vibrio parahaemolyticus.Finally,the mortality rate of E. carinicauda infected with V. parahaemolyticus after Duox gene knockdown was analyzed by using the RNA interference technique.The result shows that the coding region sequence length of the Duox gene in E. carinicauda is 4 518 bp,encoding 1 505 amino acids,reflecting typical structural characteristics of the dual oxidase.The Duox gene is expressed in all of the E. carinicauda tissues tested,with the highest expression level in gill tissues.After infected with V. parahaemolyticus,the Duox gene expression level in gills increases significantly (P<0.05).Infection with V. parahaemolyticus after injection of siRNA reagent significantly reduces the survival rate of E. carinicauda in the RNA interference group compared with the control group (P<0.05).This study shows that the Duox gene plays a role in the immune response of E. carinicauda against pathogenic infection,and provides an favorable reference for further exploration into the immune function and mechanism of Duox in E. carinicauda.
  • Research progress in the aquaculture biology of the Golden Pompano (Trachinotus ovatus)
    CHEN Huapu
    Journal of Dalian Fisheries University. 2025, 40(1): 1-11. https://doi.org/10.16535/j.cnki.dlhyxb.2024-155
    Abstract (1061) PDF (931) HTML (1)   Knowledge map   Save
    The golden pompano(Trachinotus ovatus) is a popular fish variety for marine cage culture and large-scale offshore aquaculture facilities.Successive breakthroughs in key technologies for breeding and cultivating golden pompano have promoted the rapid development of its industrial aquaculture in China.However,issues such as the decline in farming performance,caused by relatively weak fundamental biological and genetic breeding research,have become bottlenecks for sustainable development of this industry.We describe the current state of research and development in the industry and review the biological characteristics of T. ovatus,including resource distribution,habitat,morphological traits,and reproductive biology.We also summarize recent research achievements in artificial breeding,aquaculture,physiological ecology,and genetics.In light of the development,conservation,and the future potential of golden pompano resource,we propose future research directions,including selective breeding,basic genetic mechanisms,gonadal development regulation,and new strain development,to provide references for the future stable development of golden pompano aquaculture sector.
  • Optimization of enzymatic hydrolysis process conditions for Eriocheir sinensis processing by-products and its umami characteristics of enzymatic hydrolysate
    GENG Xinghua, ZHANG Jiahui, LIU Taiang, SHI Yuyao, WANG Xichang
    Journal of Dalian Fisheries University. 2025, 40(2): 250-262. https://doi.org/10.16535/j.cnki.dlhyxb.2024-186
    Abstract (1014) PDF (192) HTML (0)   Knowledge map   Save
    To utilize efficiently processing byproducts of Chinese mitten crabs (Eriocheir sinensis),we used the byproducts as raw materials,optimized enzymatic hydrolysis conditions via response surface methodology,and investigated the umami characteristics of the hydrolysate.The enzyme for the experiment was determined based on sensory evaluation,degree of hydrolysis (DH),and protein utilization rate.Using DH as the evaluation index,the optimal enzymatic hydrolysis conditions were established via response surface methodology,and the umami-enhancing effect of the hydrolysate under these conditions was assessed.The results showed that the optimal enzymatic hydrolysis conditions were:hydrolysis time of 4 h,enzyme dosage of 1 609 U/g substrate,temperature of 46 ℃,and pH 6.0.Under these conditions,the DH reached 20.47%.The content of umami amino acids and flavor nucleotides in the hydrolysate increased by 33.83 mg/100 g and 18.33 mg/100 g,respectively,compared to pre-hydrolysis.Electronic tongue analysis confirmed a significant increase in umami intensity (P< 0.05) after hydrolysis,and sensory evaluation indicated a pronounced umami flavor,with results consistent with the electronic tongue data.These results demonstrate that enzymatic hydrolysis significantly enhances the overall umami taste of crab processing byproducts,making them suitable for developing unique umami-rich seasoning bases with distinct crab flavor.
  • Evaluation of low-salt tolerance of razor clams (Sinonovacula constricta) from different families and their physiological responses
    ZHAN Tianyu, ZHANG Na, YE Kai, BAI Zengqi, KANG Pengtian, NIU Donghong
    Journal of Dalian Fisheries University. 2025, 40(5): 780-788. https://doi.org/10.16535/j.cnki.dlhyxb.2025-068
    Abstract (817) PDF (86) HTML (0)   Knowledge map   Save
    To evaluate low salt tolerance of different family lines of razor clam (Sinonovacula constricta), we exposed specimens from six razor clam family lines (8, 13, 14, 23, 24, and 28) to low salt (2 salinity) or control (20 salinity) conditions to carry out low salt stress experiments. Tissue damage, apoptosis, Na+/K+-ATPase (NKA) activity, and antioxidant enzyme activity were measured and compared among the specimens from the different families. The results showed that the mortality rates of razor clams from F8, F13, and F14 were >80% but the survival curves of all families were similar. The Pearson correlation between razor clam survival and shell width was highly significant (P<0.01), and the Spearman correlation between shell width and body weight was significant (P<0.05). Hematoxylin and eosin (HE) staining showed that clams from F8, F13, and F14 had normal gill tissue structure by 48 h, some gill filaments were swollen at 72 h, gill spacing was reduced, gill volume was enlarged, and the area of broken region was increased at 96 h. Clams from the F23 group had slight edema of localized gill filaments at 72 h, and their damage extent were not worse than those from F8, F13, and F14 by 96 h. F24 and F28 clams had intact gill filament structure at 96 h of the stress cycle. The level of apoptosis was observed by Hoechst 33342 staining, showing that the nuclei of F8, F13, and F14 cells displayed dense bright blue patches, with aggregates of apoptotic vesicles and more apoptosis. In clams from the F23 group, the proportion of apoptotic cells was reduced; and the proportions of apoptotic cells were lower in F24 and F28 clams, with occasional sporadic apoptotic vesicles. The activity of NKA of clams from all family lines decreased continuously during 12 h of low salt stress, and the enzyme activities of clams from the F23, F24, and F28 increased significantly by 24 h but then decreased.The NKA activity levels of F8 and F13 clams decreased continuously, and the value of F8 was always lower than that of F13.The antioxidant enzyme activity assay showed that the catalase activity of two lines (F13 and F14) was always lower than that of the other lines. The superoxide dismutase activity of clams in all families increased and then decreased, with the maximum value at 24 h. The malondialdehyde content of clams in the F8, F13, and F14 groups was higher than that of clams in the F23, F24, and F28 groups at the end of the low salt stress period. In conclusion, the six razor clam families had different tolerances to low salt conditions, and the F23, F24, and F28 clams were the most tolerant. The study results provide a reference for further breeding new low salt-tolerant razor clam lines.
  • Effects of cold exposure on the immune indicators and gene expression of Ruditapes philippinarum
    CHEN Sunan, KONG Xue, SONG Manzong, CAI Yuefeng, ZHI Ying, SHEN Xin
    Journal of Dalian Fisheries University. 2025, 40(1): 12-24. https://doi.org/10.16535/j.cnki.dlhyxb.2024-121
    Abstract (696) PDF (338) HTML (0)   Knowledge map   Save
    The Manila clam,Ruditapes philippinarum,is an economically important bivalve shellfish whose survival is affected by a variety of environmental factors,temperature being a key factor affecting its normal activities.Much attention has been given to the response of shellfish to high temperature,while fewer studies have been conducted on their response to low temperature.Considering the increasing occurrence of extreme weather,such as extreme cold,research is required on the adaptation of shellfish to low temperatures.In this study,R. philippinarum was exposed to low temperature for 60 days.Its immune indicators were systematically measured and analyzed,and the differentially expressed genes (DEGs) obtained were analyzed by using the GO/KEGG enrichment databases.A total of 8 344 and 2 674 DEGs were found in the gill tissues of R. philippinarum after 20 and 60 days of cold exposure,respectively.Enrichment analysis of these DEGs revealed that R. philippinarum has potential low temperature coping strategies in biological processes such as protein processing,substance metabolism,antioxidant,immune and anti-apoptosis.Regarding protein processing,the expression of the SAR1B,HSP20,HSP70,TRX,and SEC61G genes were up-regulated during protein processing,suggesting that R. philippinarum may maintain cellular stability at low temperatures by enhancing protein synthesis.Phosphatidylcholine (PC) is a key component of cell membranes,and the up-regulated expression of PC synthesis-related genes in this study suggests that R. philippinarum may maintain cell membrane stability under cold exposure by enhancing PC synthesis.In this study,the expression of the SOD,CYP450,GST,and GPX genes were up-regulated,and the MDA content decreased when the SOD activity increased during the late stage of cold exposure,suggesting that hypothermia induces SOD to scavenge excessive ROS in the organism,thus protecting it from hypothermic injury.In addition,the immune and anti-apoptosis related genes were also significantly activated at low temperature.These results provide basic data for furtherstudy on the mechanism of low-temperature tolerance in R. philippinarum and provide theoretical support for the selection and breeding of low-temperature tolerant shellfish varieties.
  • The current development status and future prospects of smart fisheries technology:a review
    LIU Huang, LIU Shijing
    Journal of Dalian Fisheries University. 2025, 40(4): 541-551. https://doi.org/10.16535/j.cnki.dlhyxb.2025-183
    Abstract (695) PDF (803) HTML (0)   Knowledge map   Save
    Smart fisheries are an important means of improving fishery production efficiency and promoting the modernization and transformation of the fishery industry. By deeply integrating advanced technologies such as multisensing, the Internet of Things (IoT), big data, and artificial intelligence, smart fisheries have made significant progress in key areas such as breeding, aquaculture, fishing, and processing. However, the new trends in the high quality development of modern fisheries pose higher demands on the development of smart fisheries. This paper systematically reviews the major research progress in smart fisheries technologies from three dimensions: information sensing, transmission, and analysis and decision-making. It also outlines the technology development trends and summarizes the current applications of intelligent technologies in breeding, aquaculture, resources, fishing, and processing, in combination with the practical needs of the industry. The aim is to provide support for the transformation of China’s fisheries from a big fishery country to a strong fishery nation.
  • Design of a water quality monitoring system for shellfish purification and temporary rearing
    PAN Lanlan, CHEN Kairui, YU Hankun, ZHOU Yangtao, LIU Hongyuan, ZHENG Yu, FU Xinyuan, ZHANG Guochen
    Journal of Dalian Fisheries University. 2025, 40(3): 510-518. https://doi.org/10.16535/j.cnki.dlhyxb.2024-197
    Abstract (660) PDF (310) HTML (1)   Knowledge map   Save
    To address the significant water quality fluctuations and delayed oxygenation caused by the high latency of the oxygenation system in the shellfish purification and temporary rearing processes, we designed and made a water quality monitoring system based on the NBIoT communication technology. The system uses a multi-point sensor layout to monitor water quality parameters such as temperature, pH, turbidity, and dissolved oxygen (DO) in real time, and the particle swarm optimization (PSO) algorithm optimizes the back propagation (BP) neural network and the Proportional-Integral-Derivative (PID) controller and builds predicting model for DO and controlling model of automatic aerator. The system uses an STM32 microcontroller as the main control unit for the water quality monitoring sensors,and accomplishes data collection and transmission. Remote communication and data storage have been facilitated through the NBIoT module and the Alibaba Cloud platform. Simulation results showed that the PSO-BP neural network-based dissolved oxygen prediction model had high prediction accuracy, while the PSO-PID controller has improved stability and faster response for aerator control. System testing indicated that the average error in sensor data collection was <5%, confirming high sensor data collection accuracy. This system provides comprehensive, real-time monitoring of water quality throughout the entire process of shellfish purification and temporary rearing, achieving the requirements for water quality monitoring in such applications.
  • Effect of β-carotene supplementation on growth and antioxidant ability of female swimming crab (Portunus trituberculatus)
    LI Zejun, SUN Yawen, HE Wende
    Journal of Dalian Fisheries University. 2025, 40(2): 290-297. https://doi.org/10.16535/j.cnki.dlhyxb.2024-206
    Abstract (634) PDF (116) HTML (0)   Knowledge map   Save
    Dietary supplementation with β carotene was investigated to assess its impact on gonad development,antioxidant status,color parameters,and carotenoid composition in adult female swimming crabs (Portunus trituberculatus).Four dry diets (β-0,β-50,β-100,and β-200) were formulated to contain 1.30,49.15,102.13,and 203.53 mg/kg of synthetic β-carotene,and a 60 day feeding trial was conducted.No significant differences in body weight or gonadosomatic index were observed among the groups,indicating that β-carotene did not significantly enhance the growth and gonad development of adult P. trituberculatus.The inclusion of 50-100 mg/kg of β-carotene in the diet led to improvements in antioxidant capacity indicators and immune-related parameters in both the hepatopancreas and hemolymph.Notably,the redness of the ovaries and hepatopancreas increased significantly with higher dietary β-carotene supplementation.In conclusion,incorporating β-carotene into the diet enhanced the antioxidant ability,non-specific immunity,and tissue carotenoid contents in adult female P. trituberculatus.Moreover,the results suggest that the optimal level of dietary synthetic β-carotene supplementation for adult female P. trituberculatus is around 100 mg/kg.
  • Exploring low and Ultra-low temperature preservation techniques for sperm of Hemibarbus labeo in the Yalu River
    LI Jianqiao, LUO Xiaonian, DUAN Youjian, LI Jiao, KANG Junlong, MO Yanfang, DONG Ruixin, XI Wenqiu
    Journal of Dalian Fisheries University. 2025, 40(2): 204-215. https://doi.org/10.16535/j.cnki.dlhyxb.2024-223
    Abstract (610) PDF (245) HTML (0)   Knowledge map   Save
    To preserve the genetic resources of Hemibarbus labeo in Yalu River,ten mature parent fish (♀∶♂= 4∶6) were taken from Xingda Fish Farm in Liaoyang County in May 2024.The effects of various dilution solutions,dilution ratios,cryoprotectants,and freezing and thawing protocols on sperm were assessed.Results indicated that the dilution solution L-1 (0.8 g of NaCl,0.05 g of KCl,and 0.50 g of glucose dissolved in 100 mL of distilled water) had the superior preservation efficacy for H. labeo sperm.After 4 ℃ low temperature storage for 48 h,activation rates reached 71.667%±2.887%,motility times averaged (21.667±1.528) s,and lifespans were recorded at (41.000±1.000) s.The ideal dilution ratio was 1∶5,and post-storage recovery metrics showed activation rates of 76.667%±2.887%,motility times averaging (24.000±2.000)s,and lifespans of (39.333±1.528) s after low temperature storage for 48 h with a cryoprotectant (DMSO) concentration of 10%.The optimal freezing conditions for H. labeo sperm involved exposure to nitrogen vapor approximately 3 cm above the liquid nitrogen surface for 5 min prior to thawing.This treatment resulted in high vitality levels when 24-hour preserved sperm were thawed using a water bath set to 37 ℃ for 20 s and recovery metrics showed an activation rate of 80.000%±8.667%,average motility time of (37.000±1.733)s,and lifespan of (102.333±3.215)s.Following sperm recovery from the frozen state,artificial insemination trials yielded fertilization rates and hatching success rates of 70.667%±1.154% and 72.000%±4.583%,respectively.This study establishes a comprehensive system for low temperature and ultra-low temperature preservation techniques applicable to H. labeo sperm,thereby providing foundational data essential for both germplasm resources conservation and artificial reproduction of this species.
  • Effect of low temperature silencing and resuscitation on gene transcription and expression in sperm of Glyptocidaris crenularis
    WANG Hao, WANG Yongjie, TIAN Fenglin, RUAN Shuchao, TIAN Ye, LI Shufeng, DING Beichen, DING Jun, HAN Bing
    Journal of Dalian Fisheries University. 2025, 40(2): 195-203. https://doi.org/10.16535/j.cnki.dlhyxb.2023-244
    Abstract (608) PDF (242) HTML (0)   Knowledge map   Save
    To explore the regulatory mechanism of sperm motility in the yellow sea urchin (Glyptocidaris crenularis) during cryopreservation and resuscitation,RNA-Seq was used to analyze the gene transcription level of sperm after cryopreservation and seawater resuscitation.The results showed that 5.92-6.99 Gb of data were obtained for every group of sea urchin by high throughput sequencing,and that 113 728 transcripts and 73 814 Unigenes were assembled.And 15 333 Unigene annotations were made to the Kyoto Encyclopedia of Genes and Genomes database and classified into 290 pathways.The results of differentially expressed gene enrichment showed that low temperature treatment inhibited cell gap connection communication,oxidative phosphorylation and other pathways; and enhanced carbohydrate catabolism,pentose phosphate signaling,NADPH regeneration,glucose 6 phosphate metabolism,and other pathways after seawater resuscitation.The expression levels of differentially expressed genes Spe 8,Spe 12,CatSper2,and NKAa1,which are related to the regulation of sperm motility,were verified by fluorescence quantitative PCR.Their expression levels were significantly downregulated after low temperature silencing but significantly increased after seawater resuscitation.These results provide a theoretical basis and new clues for further research on the effects of cryopreservation and resuscitation on the regulatory mechanism of yellow sea urchin sperm.
  • Comparative transcriptomics reveals key genes and underlying mechanisms of salinity-alkalinity tolerance in Gymnocypris przewalskii
    YAN Qingchun, LI Changzhong, WANG Linan, YANG Ying, LI Zhaonan, LIANG Tianxiu , JIN Wenjie, CHEN Yanxia
    Journal of Dalian Fisheries University. 2025, 40(2): 223-234. https://doi.org/10.16535/j.cnki.dlhyxb.2024-200
    Abstract (583) PDF (327) HTML (0)   Knowledge map   Save
    To further explore the regulatory mechanism of Gymnocypris przewalskii to adapt hypersaline and hyperalkaline water environment,we screened the genes that may play important regulatory roles in its adaptation to the hypersaline and hyperalkaline water environment of Qinghai Lake.We used transcriptomics technology to sequence the liver (GECL,GPRL),kidney (GECK,GPRK),and gill (GECG,GPRG) tissues of G. przewalskii and G. eckloni using high throughput sequencing.The different tissues of the two species were screened for differentially expressed genes (DEGs),which then were functionally annotated.The results showed that a total of 149.57 Gb of clean data were obtained from 18 samples,that the clean data from each sample reached >6.50 Gb,that the percentage of Q30 bases was >83.89%,and 224 010 Unigenes were totally obtained after de novo assembly.The GECG vs GPRG,GECK vs GPRK,and GECL vs GPRL comparisons yielded 9 807,6 550,and 5 651 DEGs respectively.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that DEGs co-expressed in gill,kidney,and liver tissues of G. przewalskii and G. eckloni were significantly enriched in the signaling pathways of “ferroptosis”,“apoptosis”,and “glycolysis/gluconeogenesis” (P< 0.05).The DEGs annotated to these pathways included genes such as tfa,tfr1a,atf4b,and hk2,which may play important regulatory roles in the adaptation of G. przewalskii to the hypersaline and hyperalkaline water environment of Qinghai Lake.
  • Structure and expression characteristics of taurine transporter CgTauT genes in Crassostrea gigas
    JIANG Chunyu, KONG Ning, SUN Shiqing, LI Xiang, LUO Cong, QIAO Xin, WANG Lingling, SONG Linsheng
    Journal of Dalian Fisheries University. 2025, 40(1): 25-33. https://doi.org/10.16535/j.cnki.dlhyxb.2024-125
    Abstract (581) PDF (297) HTML (0)   Knowledge map   Save
    To investigate the composition and expression characteristics of the taurine transporter (CgTauT) gene family in Crassostrea gigas,a genome-wide screening strategy was used to identify taurine transporter genes,and the qRT-PCR was used to analyse their tissue distribution pattern and to expression characteristics after taurine treatment.The results revealed that C. gigas contains four CgTauT genes,with each containing an SNF structural domain and 12 transmembrane domains.The four CgTauT genes were constitutively expressed in the various tissues of C. gigas,with CgTauT1 showing the highest expression in the adductor muscle,and CgTauT2,CgTauT3 and CgTauT4 showing the highest expression in the mantle.There were no significant correlations between the expression levels of the four CgTauT genes and the taurine contents of the tissues.Under taurine immersion treatment,the four CgTauT genes exhibited different temporal expression profiles in the mantle of C. gigas.The expression level of CgTauT1 increased after three hours of treatment.With prolonged taurine treatment,the mRNA expression level of CgTauT3 increased gradually,while that of CgTauT4 first increased and then decreased.These findings provide a theoretical foundation for further exploration of the physiological functions of CgTauTs and the molecular mechanisms underlying taurine accumulation in C. gigas.
  • Glucose metabolism and relevant response of Gymnocypris przewalskii exposed to chronic alkalinity stress
    GAO Zihan, WEI Wei, YUE Miao, LI Yixin, CUI Yanrong, XU Baoke, LI jin, WEI Fulei, LIANG Jian
    Journal of Dalian Fisheries University. 2025, 40(1): 65-75. https://doi.org/10.16535/j.cnki.dlhyxb.2024-042
    Abstract (563) PDF (178) HTML (0)   Knowledge map   Save
    To inquire mechanism of Gymnocypris przewalskii adaptation to alkalinity changes through glucose metabolism,G. przewalskii with body weights of 126.67 g±8.88 g were reared in an 85 cm×56 cm×62 cm tank containing either freshwater (control group) or four experimental alkalinities of 25%,50%,75% and 100%,prepared using NaHCO3 and Na2CO3 dissolved in tap water (NaHCO3∶Na2CO3 = 9∶1).The important indicators of G. przewalskii glucose metabolism were determined,including glycogen content,glucose (GLU) content,and the activities of pyruvate (PA),hexose kinase (HK),pyruvate kinase (PK),phosphoenolpyruvate (PK),phosphoenolpyruvate carboxylase (PEPCK),glucose 6 phosphate dehydrogenase (G6PD),and fructose 1,6 bisphosphate aldolase (FBA).The results showed that,with increasing alkalinity,the hepatic glycogen,muscle glycogen,and GLU content in the gills and kidneys increased and then decreased,and the GLU in the muscle gradually decreased.As the alkalinity increased,PA in the gills increased and then decreased,and PA in the kidneys,liver,and muscle decreased and then increased.The activities of HK,PK,PEPCK and G6PD in the gills showed an increasing trend with increasing alkalinity,and the activities of FBA in the kidneys and liver gradually rose.A heatmap showed that glucose metabolism processes in the gills,kidneys,and liver were promoted by increasing alkalinity to some extent,while glucose metabolism in the muscle was inhibited as alkalinity increased.As alkalinity increased,fba-c was commonly expressed in the gills,kidneys,intestine,and liver,and the expression level of fba-c in the gills and kidneys increased and then decreased.The maximum expression levels were observed in the 75% alkalinity group.In conclusion,the gills,kidneys,and liver responded to alkalinity changes by increasing glucose metabolism,while muscle responded to alkalinity changes by inhibiting glucose metabolism,leading to rapid adaptation to different alkalinity levels in G. przewalskii.
  • Analysis on karyotype of F1 hybrids of red seabream(Pagrus major)♂×black porgy (Acanthopagrus schlegelii)♀ and F1 self-fertilization of black porgy
    ZHOU Shimiao, XU Dafeng, DU Shuran, JIA Chaofeng, MENG Qian, ZHU Fei, CHEN Shuyin
    Journal of Dalian Fisheries University. 2025, 40(2): 216-222. https://doi.org/10.16535/j.cnki.dlhyxb.2024-212
    Abstract (526) PDF (169) HTML (0)   Knowledge map   Save
    To analyze the chromosome karyotype changes in F1 self-fertilization of Acanthopagrus schlegelii (AS) and F1 hybrids of AS ♀× Pagrus major (PA) and to provide a basis for black porgy breeding,metaphase chromosome morphology of two sub-populations were prepared respectively by injecting phytohemagglutinin(PHA) and colchicine.The results showed that the chromosome number of both AS and PA is 2n = 48.The karyotype of AS is 2n = 4m + 4sm + 2st + 38t (NF = 56),while the karyotype of PA is 2n = 8sm + 2st + 38t (NF = 56).They have some different karyotypes and st chromosomes with distinct satellite structures.The karyotype of AS is completely consistent with that of the black porgy female parent,while the karyotype of PA is inconsistent with that of the parent species.The number of st and t type chromosomes in the karyotype of PA is the same as that of the female parent,and total number of m+sm type chromosomes is also same.This might suggest gynogenesis for PA.The study provides reference information for the genetics and breeding of F1 hybrids and F1 self-fertilized black porgy.
  • Application of computer vision to recognize fish behavior
    GUO Jianjun, CHEN Jiexin, LIN Lijun, KONG Yiyou, YE Junwei, XU Xin
    Journal of Dalian Fisheries University. 2025, 40(2): 351. https://doi.org/10.16535/j.cnki.dlhyxb.2024-210
    Abstract (522) PDF (221) HTML (0)   Knowledge map   Save
    Understanding fish behavior is beneficial to both aquaculture management and farmers.In recent years,the application of computer vision has developed rapidly in the field of fish recognition,and it provides a non-contact machine detection method for recognizing fish behavior.This technology integrates advanced technologies in many fields,such as image processing and machine learning.Through frame analysis and processing of captured fish behavior videos,key frame image information is extracted to realize automatic recognition and quantitative analysis of fish behavior patterns.Results of this study can provide guidance and inspiration for future research and promote the further application and development of computer vision technology in fish behavior research.
  • Screening and identification of a highly efficient denitrifying functional bacterium XYB4 and its effects on the growth and intestinal flora of Litopenaeus vannamei and water quality
    WU Jiaqi, ZHOU Jianing, XUE Yuan, WANG Furui, ZHU Miao, SUN Chengbo
    Journal of Dalian Fisheries University. 2025, 40(1): 86-94. https://doi.org/10.16535/j.cnki.dlhyxb.2024-126
    Abstract (521) PDF (189) HTML (0)   Knowledge map   Save
    To develop highly efficient and economic denitrifying technology for an aquaculutre system,the experiment isolated a highly efficient denitrifying bacterium from a biofloc in shrimp culture pond ( named as XYB4) by morphological screening,physiological and biochemical identification,molecular biological assay,safety evaluation test,and denitrifying effect under the influence of different physical and chemical factors.The result shows that the XYB4 strain is a gram-positive bacillus.16S rDNA sequence result shows that it is Bacillus subtilis.The safety evaluation test shows that XYB4 is not lethal to Litopenaeus vannamei,without hemolysis,and is sensitive to 14 types of antibiotics.The best conditions for denitrification by the XYB4 strain are as follows:salinity 30,temperature 30 ℃,pH 7.5,and C/N 10-15.A mixture of XYB4 with feed (B7),splashed into an aquaculture pond (P7),significantly reduced the concentrations of  and  in the cultured water (P<0.05),and significantly increased the growth rate and specific growth rate of L. vannamei by 16.32%,17.52%,and 40.18%.and 40.06%,respectively.There was no significant difference in survival rate among the three groups (P>0.05).The richness of the L. vannamei intestinal flora and the water flora is significantly increased in the experimental group (P<0.05).The addition of the XYB4 strain inhibited the growth of harmful bacteria,such as Photobacterium and Tenacibaculum,and promoted the growth of beneficial nitrifying bacteria,such as Nitrospirae.In conclusion,the application of XYB4 can effectively reduce the concentrations of  and  in aquaculture water,promote the growth of L. vannamei,improve the richness of the microflora communities in the water and L. vannamei intestines,and inhibit the growth of some harmful microbial populations.The XYB4 has a high application value in aquaculture production.
  • Fish biodiversity survey and assessment in the Estuary of Liaohe River with the environmental DNA metabarcoding technology and bottom trawl sampling method
    WANG Xiaolin, YAN Long, JI Guang, DONG Jing, XU Yanzhao, WANG Aiyong, LIU Xiuze, WANG Bin, KONG Yefu
    Journal of Dalian Fisheries University. 2025, 40(2): 298-307. https://doi.org/10.16535/j.cnki.dlhyxb.2024-105
    Abstract (506) PDF (167) HTML (0)   Knowledge map   Save
    To evaluate fish diversity in the Liaohe Estuary and adjacent sea at autumn and to explore the applicability of environmental DNA (eDNA) metabarcoding in monitoring fish diversity,both seawater samples for eDNA and fish samples by bottom trawl survey were taken at ten survey stations in the Liaohe Estuary and adjacent sea in October 2023.High throughput sequencing and biological analysis were conducted separately.In total,37 fish species were detected in the eDNA sample ASV annotation results,2 of which were identified only at the genus level,and 16 fish species were identified in the fish samples by bottom trawl survey.13 fish species were totally identified by these two methods.The number of fish species detected by the eDNA metabarcoding technology was 2.3 times more than that by the bottom trawl survey.The differences in alpha diversity indices among the different stations based on the eDNA detection data were not significant.Principal component analysis (PCA) showed that the first two principal components could explain 57.04% of differences in fish communities (PC1 43.04%,PC2 14.0%),and there was little difference in fish community composition among sampling stations.Pearson correlation analysis showed a high correlation between the relative abundance of eDNA and relative catch (biomass,number of individuals) of bottom trawl surveys,with r values of 0.921 and 0.888,respectively,and the correlation was highly significant (P< 0.01).Redundancy analysis(RDA) results showed that there was no significant correlation between various water environmental factors (temperature,salinity,pH,DO,water depth) and fish community structure (P> 0.05).In summary,the eDNA technology had higher species detection efficiency than bottom trawl survey method,and there was a significant correlation between the relative abundance of the eDNA and the relative catch,indicating that the eDNA technology can be effectively applied to monitor and evaluate fish diversity in the Liaohe Estuary and its adjacent waters.
  • Effects of ocean warming on bacterial biofilm formation and biofilm-induced larval metamorphosis of the mussel Mytilus coruscus
    MA Fan, TAO Yu, YANG Jinlong, LIANG Xiao
    Journal of Dalian Fisheries University. 2025, 40(2): 241-249. https://doi.org/10.16535/j.cnki.dlhyxb.2024-205
    Abstract (505) PDF (160) HTML (0)   Knowledge map   Save
    The goal of this study was to assess how ocean warming regulates biofilm formation and larval metamorphosis of the mussel Mytilus coruscus. Pseudoalteromonas marina,a typical marine bacterium known for inducing larval metamorphosis,was selected as the test organism.The temperature gradient of 14 ℃,18 ℃,22 ℃,27 ℃,and 31 ℃ was set based on the sea surface temperature of the ocean combined with ocean warming.The characteristics of biofilms formed at different temperatures and their induction of larval metamorphosis were observed.The results showed that bacterial density,extracellular a-polysaccharide and cyclic dimeric guanosine monophosphate contents (c-di-GMP),and induction activity increased with increasing seawater temperature to a peak at 22 ℃,and then they decreased as temperature increased.The induction activity of biofilms was not correlated with β-polysaccharide content but was correlated with c-di-GMP content.Furthermore,the variation trends of lipopolysaccharide and colanic acid contents were consistent with the changes in biofilm induction capacity,whereas cellulose content showed an increasing trend with rising temperature.As c-di-GMP content regulates secretion of extracellular polysaccharides,temperature induced changes in its contents ultimately affect the capacity of the biofilm to induce larval metamorphosis.These results can be applied to improving the larval settlement efficiency of mollusks under ocean warming and to the sustainable development of the marine mollusk aquaculture industry.
  • Cloning the OSCP subunit gene of Macrobrachium rosenbergii and assessing its influence on the structure of ATP synthase
    CHEN Xin, DAI Xilin, JIA Chuipan, DONG Wangming, HE Bo, LUO Zhenhui, BU Xiaoyang
    Journal of Dalian Fisheries University. 2025, 40(3): 363-371. https://doi.org/10.16535/j.cnki.dlhyxb.2024-170
    Abstract (503) PDF (302) HTML (4)   Knowledge map   Save
    To investigate the role of the oligomycin sensitivity-conferring protein (OSCP) subunit gene of ATP synthase in the structural integrity and functional stability of ATP synthase in the freshwater prawn Macrobrachium rosenbergii, its full-length cDNA was cloned by using rapid amplification of cDNA ends(RACE) technology. Small interfering RNA (siRNA) was used to interfere with the expression of the OSCP subunit gene, and the resulting changes in the expression levels of other ATP synthase subunit genes were explored. The results showed that the full-length cDNA of the OSCP subunit gene of ATP synthase in M. rosenbergii is 922 base pairs long and encodes a hydrophobic protein consisting of 209 amino acids. Homology comparison and genetic relatedness analysis indicated that the OSCP subunit gene in M. rosenbergii has the highest homology and closest genetic relationship with the American lobster (Homarus americanus). Quantitative PCR analysis of tissue samples revealed that the expression level of the OSCP subunit gene was highest in the heart. The optimal injection dose of siRNA was 1.2 μg/g. When the expression of the OSCP subunit gene in the heart of M. rosenbergii was downregulated, the expression levels of the α, β, γ, δ, b, and d subunit genes of ATP synthase decreased significantly (P<0.05). This result indicates that inhibition of the expression of the OSCP subunit gene affects the expression of other subunit genes, thereby influencing the synthesis of ATP synthase.
  • Study on the response of polian vesicle to Vibrio splendidus invasion in Apostichopus japonicus
    JIA Ziqiang, LI Qiang, REN Yuan, WU Jiong, WANG Yinan
    Journal of Dalian Fisheries University. 2025, 40(2): 235-240. https://doi.org/10.16535/j.cnki.dlhyxb.2024-198
    Abstract (499) PDF (187) HTML (0)   Knowledge map   Save
    To investigate the response of polian vesicles to pathogenic bacteria invasion in the sea cucumber Apostichopus japonicus,we injected Vibrio splendidus,the pathogen responsible for “skin ulcer syndrome”,into the coelom of A. japonicus via the body wall,and studied the morphological and tissue structural changes of polian vesicles,and proliferation rate of cells in polian vesicles’ wall and cavity.The results showed that the morphology and tissue structure of polian vesicles changed significantly after invaded by V. splendidus.During the 2 h to 12 h after injection of Vibrio splendidus,polian vesicles expanded and elongated gradually,the number of dark-stained cells in the polian vesicles’ wall first decreased,then increased,but decreased,then increased again,and their proliferation was most active within 6 h to 12 h after injection.The 5-ethynyl-2′-deoxyuridine (EdU) positive rate of cells in the cavity of polian vesicles was 10.39%,11.20% and 11.61%,respectively,at 4 h,6 h,and 12 h after injection,whereas it was 7.22%,6.24%,and 6.61%,respectively,in the control group.These results indicated that polian vesicles responded to V. splendidus invasion by accelerating cell proliferation in the cyst wall and cavity,even if the bacteria could not enter the vesicles.
  • Study on the expression pattern of Cathepsin B in Yesso scallops (Patinopecten yessoensis) infected with Polydora
    HANG Yunna, SUN Hongyan, TANG Anqi, MAO Junxia, TIAN Ying, WANG Xubo, HAO Zhenlin, DING Jun, CHANG Yaqing
    Journal of Dalian Fisheries University. 2025, 40(1): 55-64. https://doi.org/10.16535/j.cnki.dlhyxb.2024-110
    Abstract (495) PDF (175) HTML (0)   Knowledge map   Save
    Cathepsin B (CatB) is a lysosomal cysteine protease with important immune functions.To investigate the molecular function of CatB in Yesso scallops (Patinopecten yessoensis) infected by Polydora,genomic identification,sequence characterization,and gene expression analyses of the CatB gene were conducted in P. yessoensis (PyCatB),and the changes of PyCatB enzyme activity and gene expression pettern were analyzed.The results showed that the full length of the PyCatB gene was 9 267 bp with nine exons and eight introns.The cDNA length of PyCatB was 1 219 bp,with a 5′UTR of 103 bp and a 3′UTR of 99 bp,and an open reading frame length of 1 017 bp,encoding 338 amino acids.PyCatB was predicted to contain three conserved functional domains; the N-terminal signal peptide; Propeptide C1; and peptidase C1A cathepsin B.Additionally,it also had a catalytic triplet structure (Cys116,His284,Asn304),a Gln110 oxygen anion pore,a GCNGG motif,and a characteristic occluding loop formed by C194/C205 fixed residue fragments,which played important roles in the catalytic activity of PyCatB.Phylogenetic analysis showed that the evolutionary status of CatB was consistent with the species taxonomy.Enzyme activity analysis showed that the enzyme activity of CatB in the mantle tissue of P. yessoensis infected with Polydora was significantly higher than that in healthy scallops.Gene expression analysis showed that PyCatB was expressed at different levels in the various tissues of healthy adult scallops except for the adductor muscle,indicating that PyCatB was involved in a wide range of physiological processes.The expression levels of PyCatB in different mantle regions of infected scallops were all significantly higher than those in healthy scallops,but there was no significant difference between the hemocytes of healthy and infected scallops.It was speculated that PyCatB probably acted mainly through the mantle tissue to participate in the immune response processes of P. yessoensis to resist Polydora infection.This study provides a reference for analyses of the mechanism whereby P. yessoensis resists Polydora infection and lays an important theoretical foundation for Polydora infection control in molluscs.
  • Diurnal variation of shell opening and closing frequency and heart rate of the mussel Hyriopsis cumingii and the responses of these parameters to feeding and immune stress
    CHEN Yang, LÜ Xuefeng, LU Tingting, WANG Zhiyan, FU Baicheng, YUAN Yiping, BAI Zhiyi
    Journal of Dalian Fisheries University. 2025, 40(2): 263-270. https://doi.org/10.16535/j.cnki.dlhyxb.2024-173
    Abstract (491) PDF (131) HTML (0)   Knowledge map   Save
    To explore the feasibility of using the frequency of shell opening and closing in the mussel Hyriopsis cumingii as an indicator of organism health,we evaluated the diurnal variation of the frequency of this behavior and the heart rate of H. cumingii.We then compared the response characteristics of shell opening and closing frequency and heart rate to feeding and immune stress in the same population under normal culture conditions.The results showed that the opening and closing frequency and heart rate of H. cumingii at night were higher than those in the day,but the difference was not significant (P>0.05).The values at 1 h after feeding were significantly higher than those before feeding (P<0.05) and the heart rate amplitude increased,indicating that the shell opening and closing frequency responded to feeding behavior.The shell opening and closing frequency reached the highest value of 18.75 times/h after infection with Aeromonas hydrophila,which was 1.52 times higher than the frequency before infection (P<0.05),but by 15 h it had decreased to 31.29% of the shell opening and closing frequency before infection.At 5 h after infection,the heart rate was significantly higher than the normal level and reached a peak of 14.65 beats per minute,which was 1.25 times higher than the initial heart rate (P<0.05),and then it slowly decreased and returned to the normal level after 12 h.These results indicate that shell opening and closing and heart rate responded to bacterial infection and that the response of shell movement frequency was stronger than that of heart rate.Under normal culture conditions,shell opening and closing frequency varied within the same population,and individuals could be divided into high,medium,or low frequency.The expression of four immune-related enzymes was highest in the hepatopancreas.Expression of superoxide dismutase was significantly higher in the high frequency group compared to the low frequency group.The activities of alkaline phosphatase,acid phosphatase,and catalase in the high frequency group were significantly higher than those in the medium and low frequency groups (P<0.05).In summary,shell opening and closing frequency is more sensitive to environmental changes than heart rate,and the activities of the measured immune-related enzymes are higher in specimens that exhibit high shell opening and closing frequency.These results suggest that the frequency of shell opening and closing can be used as a health indicator for H. cumingii,which will be of great significance for promoting healthy shellfish culture.
  • A formation control method for multiple underwater robots to assess living resources in the marine ranch
    JIN Zhaoyi, SONG Jinghan, YU Yifan, WANG Xin, LU Chun, YANG Zhiqing, LI Zhijun, LIN Yuanshan
    Journal of Dalian Fisheries University. 2025, 40(2): 318-329. https://doi.org/10.16535/j.cnki.dlhyxb.2024-135
    Abstract (466) PDF (95) HTML (0)   Knowledge map   Save
    Most existing formation control methods for underwater robots rely on precise multi-underwater robot kinematics or dynamics models.As an alternative,we propose a multi-underwater robot formation control method based on the multi-agent deep deterministic policy gradient.In this model,N underwater robots are divided into one leader and N-1 followers,and a Markov decision process model based on the leader-follower architecture is established to model the formation control of the multi-underwater robots.Next,each robot’s own position and velocity information along with its position information relative to the other robots are used as observations,and their actions are represented by the forces acting on the robots.Finally,a reward function is constructed based on the distance between the leader and the target position,the distance between the followers and the leader,and the distances between the followers,which balances both individual and system-level rewards.Application of this model showed that the multi-underwater robot formation control strategy can generate cooperative motion patterns similar to those of biological swarming and outperform other deep reinforcement learning algorithms in terms of formation stability and path optimization.This strategy does not require precise kinematic or dynamic modeling,and it exhibits flexible behavior,strong distributed execution capability,and good generalization ability.It is an effective multi-underwater robot formation control method that can provide new technical support for stock assessment in the marine ranch.
  • An experimental study of an in situ labeling technique for Mactra veneriformis
    GAO Jin, XIE Xi, BAI Yongan, PANG Jinchi, LIU Xiangfeng, TENG Weiming, WANG Qingzhi
    Journal of Dalian Fisheries University. 2025, 40(1): 44-54. https://doi.org/10.16535/j.cnki.dlhyxb.2024-096
    Abstract (460) PDF (182) HTML (0)   Knowledge map   Save
    The use of calcein as an in situ labeling tool for Mactra veneriformis was examined.The optimal labeling concentration and immersion time were explored by using indicators such as the individual survival rate,the success rate of fluorescence labeling,the rate of wel-labeled individuals,and the antioxidant enzyme activity in the digestive glands and lipid markers in the soft tissues.An one-month in situ labeling experiment was conducted in Geligang in the Liaodong Bay,based on the most suitable labeling conditions.The result shows that two labeling schemes,0,25,50,75 mg/L calcein immersion for 1.5 hours and 0.50 mg/L calcein immersion for 0.5 and 1.5 hours,had no significant negative impacts on M. veneriformis.Except for the well-labeled rate of 54.90%±8.99% for the 25 mg/L calcein immersion for 1.5 hours group,the success rate and well-labeled rate of all other treatment groups were 100%.This indicates that calcein has the ability to mass-label M. veneriformis in a short period of time.The treatment involving immersion in 50-75 mg/L calcein for 0.5 to 2 hours could serve as a method for in situ labeling of M. veneriformis in intertidal flats.The results of the in situ labeling experiment indicate that immersion in 75 mg/L calcein for 2 hours produces the best results,with an average recapture rate of 10.00%±3.33% after one month,a success rate of 36.11%±12.73%,and a well-labeled rate of 100%.These results provide scientific references for the development of in situ labeling techniques for intertidal shellfish and the conservation of native high-quality clam resources.
  • A study on the underwater fish recognition method based on deep subtle features capture
    CHEN Yuwei, ZHU Xiaolong, LI Chaofan, CHU Wenjing, CHEN Zongyi, ZHANG Zhenyu
    Journal of Dalian Fisheries University. 2025, 40(3): 489-498. https://doi.org/10.16535/j.cnki.dlhyxb.2024-204
    Abstract (460) PDF (320) HTML (1)   Knowledge map   Save
    We proposed a deep subtle feature extraction-based underwater fish recognition method (DFFE-Net) to enhance the recognition capability in the complex underwater environments. This method uses YOLOv8 as the base model due to its robustness and deformable convolution DCNv4 to replace traditional convolutions, forming the EDCN module to capure, subtle features from deep characteristic image. The Shape IoU algorithm is employed to optimize the positioning of bounding boxes, thereby enhancing the precision of fish localization. Comparative experiments using a self-built underwater fish image dataset were conducted to test the model. The experimental results indicated that the proposed DFFE-Net can enhance the mAP_50, mAP_75, and overall mAP of underwater fish recognition to 95.1%, 87.2%, and 80.3%, respectively, without increasing the parameter count or computational load. Thus, DFFE-Net can provide technical support for intelligent and precise.
  • Development status and future trends in aquaponics technology：a review
    TANG Guangjing, WU Zhengli, SUN Yu, PU Decheng, LI Yanhong
    Journal of Dalian Fisheries University. 2025, 40(3): 529. https://doi.org/10.16535/j.cnki.dlhyxb.2024-183
    Abstract (449) PDF (308) HTML (0)   Knowledge map   Save
    Aquaponics is a multidisciplinary agricultural technology with the microorganisms as the common productive base. It uses the theory of circular economy to address the problem of environmental pollution and food safety and pursues the goal of green, healthy, and sustainable development in the view of ‘all encompassing approach to food’ concept. Driven by the digital transformation and intelligent applications in recent years, aquaponics technology has experienced significant advances, but still faced many problems and challenges. The paper reviewed the domestic and foreign scientific literature, explored the technical origin of aquaponics development, took the research status of the technical model as the logical line, and clarified the key technical elements, cost-bebefit feasibility, of aquaponics. The technical bottlenecks of aquaponics were analyzed, including limitations relating to salt-alkali water cultivation and breeding varieties, deficiencies in system design, and inadequate supply of artificial light sources.Additionally, challenges such as disease prevention, incomplete purification of recycled water, and a shortage of skilled personnel were also addressed. In view of the analysis above, future research directions should focus on the selection of salt-alkali water cultivation varieties, the transforming mechanisms of nitrogen, phosphorus, and other nutrients, disease prevention and control, and the RD of organic feed. It is forseeable tht that the aquaponics technology will make progress in digitization, artificial intelligence, three dimensionality, and commercialization. This may provide reliable theoretical support for promotion and application of aquaponics technology.
  • Vitamin C mitigated the effect of hexavalent chromium-induced intestinal microbiota in zebrafish (Danio rerio)
    LU Guanling, NING Jing, RUAN Yuqi, WEI Lu
    Journal of Dalian Fisheries University. 2025, 40(3): 431-439. https://doi.org/10.16535/j.cnki.dlhyxb.2024-141
    Abstract (449) PDF (198) HTML (1)   Knowledge map   Save
    Hexavalent chromium [Cr(Ⅵ)] is a common and highly toxic environmental pollutant. In this study, we assessed the effects of long-term exposure to environmental concentrations of Cr(Ⅵ) on the intestinal microflora of adult zebrafish (Danio rerio) as well as the effectiveness of the natural antioxidant vitamin C (VC) in preventing chromium oxidative toxicity. Four treatment groups were set up: control (Con) group, VC group (2 mg/L), Cr group (2 mg/L), and Cr+VC group (2 mg/L). After 60 days of semi-static aqueous phase exposure, zebrafish intestines were dissected and feces samples were taken for 16S rRNA high throughput sequencing to analyze the diversity and composition of microbial communities. The results showed that Cr(Ⅵ) induced an increase in Shannon’s index in the intestinal alpha diversity of zebrafish and that the addition of VC reduced this value to some extent (P<0.05). The beta diversity differed between groups in the major components of the gut microbiota (P<0.05). The abundance of species at the phylum and genus levels showed that Cr(Ⅵ) exposure significantly decreased the beneficial bacteria in the genus Cetobacterium (Fusobacteriota) and significantly increased the beneficial bacteria in the genus Exiguobacterium (Firmicutes) in the zebrafish intestine (P<0.05) and that the addition of VC alleviated these changes to a certain extent. Exposure to Cr(Ⅵ) exposure caused the “amino acid transportation and metabolism” function to be slightly lower than that of the Con group, but this function increased slightly after the addition of VC. These results show that VC can alleviate the balance of zebrafish intestinal microflora induced by exposure to Cr(Ⅵ) to a certain extent. The study provides a data support for the remediation of aquatic ecosystems polluted by heavy metals, and it expands the understanding of the structure and function of the zebrafish intestinal microbiota.
  • Nutritional quality and heavy metal safety risk assessment of Chinese mitten crab(Eriocheir sinensis) and crayfish (Procambarus clarkia) cultivated in cold ponds
    WANG Shihui, SU Junjie, ZHANG Shuqi, LUO Liang, ZHANG Rui, GUO Kun, ZHAO Zhigang
    Journal of Dalian Fisheries University. 2025, 40(3): 396-405. https://doi.org/10.16535/j.cnki.dlhyxb.2024-241
    Abstract (447) PDF (209) HTML (2)   Knowledge map   Save
    To investigate the nutritional quality and heavy metal safety risk of Chinese mitten crab (Eriocheir sinensis) and the crayfish (Procambarus clarkia) farmed in cold ponds with the similar environments, the edible yield, proximate composition(moisture, crude protein, total lipid and ash), fatty acids, mineral elements, and heavy metal (Cd,Cr,Hg and Pb) in these two specieswere determined by dissection, biochemical composition analysis, area normalization, and inductively coupled plasma mass spectrometry. The results showed that the average body weight, gonadosomatic index, meat yield, and total edible yield of E. sinensis were significantly higher than those of P.clarkia (P<0.01). The moisture and crude protein contents in the hepatopancreas of E. sinensis were significantly higher than those of P.clarkia, but the crude protein content in the muscle was significantly lower (P<0.01). The total saturated fatty acid, C18:3n3 (α-linolenic acid), C22:6n3 (docosahexaenoic acid), and total 3-ω polyunsaturated fatty acid contents of E. sinensis were significantly lower than those of P.clarkia (P<0.01), whereas the total polyunsaturated fatty acid content was significantly higher (P<0.01). The contents of Na, Ca, Zn, Cu, and total minerals in the hepatopancreas of E. sinensis were higher than those in the P. clarkia, but the contents of Fe and Mn were lower. The contents of Na, Ca, Fe, Zn, Cu, Mn, Se and total minerals in the muscle of E. sinensis were higher than those in the P. clarkia, but K content was lower (P< 0.01). The contents of the heavy metal elements Cd, Cr, Hg, and Pb in the hepatopancreas and muscle of P.clarkia were slightly higher than those in. In summary, had higher body weight, gonadosomatic index, meat yield, total edible yield, and total PUFA, Na, Ca, Zn, Cu, and total mineral element contents compared to P.clarkia, whereas the edible tissues of P.clarkia had higher α-linolenic acid, docosahexaenoic acid, K contents as well as higher crude protein content in muscle. The poisonous heavy metal contents in the edible tissues of both species were lower than the national food safety standard, making them safe sources of aquatic food.
  • Immunomodulatory effects of enzymolytic products of Perinereis aibuhitensis in immunosuppressed mice
    LI Lijuan, ZHU Guoping, CAO Wenhong, CHEN Zhongqin, TAN Mingtang
    Journal of Dalian Fisheries University. 2025, 40(1): 76-85. https://doi.org/10.16535/j.cnki.dlhyxb.2024-088
    Abstract (439) PDF (134) HTML (0)   Knowledge map   Save
    The enzymolytic products of Perinereis aibuhitesis were used to explore the immunomodulatory effects of P.aibuhitensis in immunosuppressed mice.The immunosuppressed mouse model was established by using cyclophosphamide induction,and the changes in body mass indexes,immune organ indexes,splenic T lymphocyte subset,total white blood cells,and immunoglobulin concentration of mice in the untreated group,model group,and sample group (the enzymolysis products below<5 000,5 to 8 000,>8 000 in P. aibuhitensis) were measured.The results showed that the three ultrafiltration components of enzymolytic products from P. aibuhitensis could increase the immune organ indexes of mice,the number of CD3e+ on T cells in the spleen,and the concentration of immunoglobulin,all of which have particular immune functions.The high dose of enzymolytic products below<5 000 in P. aibuhitensis showed the highest efficacy and significantly increased the body mass indexes,immune organ indexes,and total white blood cells (P<0.05) of the mice.And the number of CD3e+ and the ratio of CD4+/CD8a+ were significantly increased (P<0.01) and the effect of plasma cells on immunoglobulin secretion was enhanced.Perinereis aibuhitensis can ameliorate the immune impairment induced by immunosuppressants and enhance the immunomodulatory effect in immunosuppressed mice and therefore has the potential for development as an immunomodulator.
  • Prediction of bigeye tuna (Thunnus obesus) fishing ground in the Atlantic Ocean based on the modified XGBoost model
    ZHOU Shuting, ZOU Xiaorong, ZOU Bangyu
    Journal of Dalian Fisheries University. 2025, 40(3): 481-488. https://doi.org/10.16535/j.cnki.dlhyxb.2024-254
    Abstract (438) PDF (177) HTML (1)   Knowledge map   Save
    To predict accurately the bigeye tuna fishing ground and thus to reduce fishing cost effectively, we utilized the fishing operation data on the Chinese tuna longline fishing vessels in the Atlantic during the period of 2018 to 2021 and marine environmental data concurrently. Based upon the Extreme Gradient Boosting (XGBoost) model, we incrementally integrated Convolutional Neural Network (CNN) models and the Simulated Annealing (SA) algorithm to build XGBoost, CNN-XGBoost, and CNN-SA-XGBoost models to predict bigeye tuna fishing grounds in the Atlantic Ocean and compared the predictive performance of these models. The results indicated that the CNN-SA-XGBoost model achieved an accuracy (ACC) of 82% and an area under the receiver operating curve (AUC) of 0.81, which represents 15% and 6% increases in accuracy and 0.10 and 0.02 increases in the AUC compared with the XGBoost and CNN-XGBoost models, respectively. In the analysis of the importance of environmental factors by CNN, chlorophyll concentration (Chl-a), surface seawater temperature (T0), surface seawater salinity (S0), and 300 m depth salinity (S300) accounted for approximately 51% of the relative importance index, identifying them as key environmental factors affecting the catch and distribution of bigeye tuna. In summary, the CNN-SA-XGBoost model demonstrates strong accuracy, balance, and adaptability, suggesting it suitable for forecasting bigeye tuna fishing grounds in the Atlantic Ocean.
  • Fish feeding intensity recognition based on multi-modal background suppression
    WANG Hao, WANG Miao, LONG Jinlong, HAN Aihui, WU Junfeng, SUN Qunwen, YU Hong
    Journal of Dalian Fisheries University. 2025, 40(3): 499-509. https://doi.org/10.16535/j.cnki.dlhyxb.2024-174
    Abstract (434) PDF (200) HTML (1)   Knowledge map   Save
    To achieve efficient, accurate, and intelligent fish feeding andto reduce labor intensity and farming cost, this paper proposes a fish feeding intensity recognition method based on a cross-modal background suppression network (CMBS).The CMBS model integrates a cross-modal co-attention mechanism, a multi-head attention fusion module, and both temporal- and event-level background suppression modules. These components enhance the model’s focus on salient features and reduce the influence of transient noise and audio-visual asynchrony often found in single-modality data. To evaluate the effectiveness of the proposed approach, verifying experiments were conducted on two datasets： the publicly available AFFIA3K dataset (collected under controlled laboratory conditions) and a self-constructed dataset obtained from a recirculating aquaculture system (RAS), representing a real-world commercial farming environment. The proposed CMBS model was compared with seven state-of-the-art deep learning models. Experimental results show that the CMBS model achieved a three-class feeding intensity classification accuracy of 98.33% using the AFFIA3K dataset, outperforming traditional single-modal approaches. Using the Pseudocaranx dentex dataset collected in the RAS environment, the model reached an accuracy of 92.20%. These results demonstrate that the CMBS network can achieve high classification performance, and exhibits strong robustness and stability in complex, real-world aquaculture environments.
  • The influence of light intensity and shelter on habitat and feeding behavior of the spiny lobster (Panulirus homarus)
    TONG Yanling, LIANG Huafang, WEN Chongqing, WANG Chenggui, LI Zhuobo, QING Haipeng
    Journal of Dalian Fisheries University. 2025, 40(3): 382-389. https://doi.org/10.16535/j.cnki.dlhyxb.2024-285
    Abstract (433) PDF (240) HTML (2)   Knowledge map   Save
    To understand the light adaptation ability and preference for shelters of the spiny lobster (Panulirus homarus), specimens were exposed to different light intensities, light colors, and shelters. The results showed that P. homarus preferred to inhabit low light environments, demonstrating negative phototaxis. They also exhibited a preference for certain light colors. The highest habitation rate (58.61%) occurred in the red light environment and was significantly higher than those of the other three light color groups (P<0.05). Light intensity affected feeding, with greater light intensity resulting in longer time required for the feeding rate to recover. For the 300 lx light intensity experimental group, the feeding rate recovered to 101.0% on day 2, whereas it recovered to only 97.2% on day 6 in the 1 200 lx group. For the four light colors tested, the feeding rate of the lobsters returned to normal levels on day 2. P. homarus showed a preference for low height shelters. The highest habitation rate (66.48%) occurred in shelters located 1 cm from the bottom, and this rate was significantly different from those of the other groups (P<0.05). The lowest habitation rate (0.74%) was recorded in 80 cm high shelters. The lobsters also showed a preference for larger shelters. The highest habitation rate (44.76%) was detected in shelters with a diameter of 20 cm, which was significantly different those of the other groups (P<0.05). These results suggest that P. homarus prefer habitats with weak light and significantly prefer red light. For shelter selection, shelters should be placed at a bottom height not exceeding 40 cm and with a diameter ranging from 0.8 to 1.0 times the length of the antennal flagellum.
  • Structural characteristics of microbial community in the water and sediments of recirculating aquaculture systems for Apostichopus japonicus
    LIU Dan, PAN Haoran, NI Hongdong, ZHAO Xinyan, XU Tianhao, WANG Nanyi, DING Jun, CHANG Yaqing, TIAN Yi
    Journal of Dalian Fisheries University. 2025, 40(1): 135-145. https://doi.org/10.16535/j.cnki.dlhyxb.2024-133
    Abstract (425) PDF (45) HTML (0)   Knowledge map   Save
    The taxonomic composition of the microbial flora in the water and sediments of recirculating aquaculture systems for Apostichopus japonicus were studied by using 16S rRNA high-throughput sequencing in different months of year.The Alpha diversity results showed that the diversity and richness of microbial flora in the recirculating water of aquaculture tank were significantly higher than those in the sediments (P<0.05).Comparing the differences of the microbial community composition between the water and sediments,the relative abundance of Bacteroidetes in the sediments was found to be significantly higher than in the water (P<0.001),and the relative abundance of Actinomycetes in the sediments was significantly lower than in the water (P<0.05).The relative abundances of Lutibacter and Pseudoruegeria in the sediments were significantly higher than in the water (P<0.05).Regarding the monthly differences at the phylum level in the water:the proportion of Cyanobacteria was higher in May; the proportions of unidentified Bacteria and Chloroflexi were higher in July; the proportions of Octadecabacter and unidentified Cyanobacteria were higher in May; the proportion of Arcobacter was higher in July; and the proportions of Marinobacterium and Glaciecola were higher in September.Regarding the monthly differences at the phylum level in the sediments:Cyanobacteria were higher in September; Glaciecola,unidentified_Flavobacteriaceae and Octadecabacter were higher in May; Arcobacter and Agarivorans were higher in July; and unidentified Cyanobacteria were higher in September.The results showed that the dominant taxa in the water and sediments were different in different months of the year,and could provide a reference for the rational utilization of anti-microbial agents in the healthy culture of A. japonicus in recirculating water aquaculture systems.
  • Research progress and prospect of solid waste transport performance in recirculating aquaculture system:a review
    ZHAO Chenxu, WU Gang, SUN Wei, HU Wei, REN Xiaozhong, ZHANG Jun, WANG Xian
    Journal of Dalian Fisheries University. 2025, 40(1): 174. https://doi.org/10.16535/j.cnki.dlhyxb.2024-161
    Abstract (424) PDF (108) HTML (0)   Knowledge map   Save
    Recirculating aquaculture systems (RAS) are widely acknowledged as pivotal in developing future improved aquaculture practices because of their ability to rear a large number of aquatic organisms in a small space,conserve resources,and maintain environmental sustainability.Nevertheless,the buildup of solid waste in RAS presents a danger to the health of aquaculture species and has detrimental impacts on the functioning and productivity of a system.Efficient management and disposal of solid waste in RAS is crucial for maintaining optimum system operation.This study examines the categorization,origins,and potential consequences of solid waste in the RAS,and also explores methods to enhance waste transportation efficiency.Significant improvements in solid waste removal efficiency can be achieved by optimizing the design of aquaculture tanks,upgrading intake and drainage structures,modifying tank bottom gradients,utilizing diversion devices,and enhancing feed quality.These improvements will guarantee the consistent and effective functioning of a RAS.
  • Study on application of deep learning models to forecast chlorophyll-a concentration in the Estuary of Changjiang River
    CHEN Wei, HAN Zhen
    Journal of Dalian Fisheries University. 2025, 40(2): 330-337. https://doi.org/10.16535/j.cnki.dlhyxb.2024-168
    Abstract (422) PDF (209) HTML (0)   Knowledge map   Save
    To study the role of deep learning models in forecasting chlorophyll a concentration,we did a comparative analysis of precision with the three deep learning models,namely Long Short-Term Memory (LSTM),Gated Recurrent Unit (GRU),and Convolutional Neural Network (CNN),to forecast chlorophyll a concentration in the Estuary of Changjiang River.The root mean square error (RMSE) and mean absolute percentage error (MAPE) for the GRU model were 1.452 9 mg/m3 and 15.171 6%,respectively,indicating that the GRU model can effectively capture short-term dependencies.The RMSE and MAPE for the LSTM model were 1.493 1 mg/m3 and 14.759 1%,respectively,suggesting that the LSTM model is better at capturing key long-term dependencies.The RMSE and MAPE for the CNN model were 1.756 1 mg/m3 and 16.936 6%,respectively,meaning that the CNN model performs well in analyzing feature data with spatial locality.Based on these findings,we propose an attention score calculation scheme that optimizes the GRU model and improves the forecast precision,with an overall RMSE of 0.921 9 mg/m3 and a MAPE of 11.209 4%.
  • Effects of glass microbeads on the behavior, gut morphology, and gut microbiota of sea cucumber (Apostichopus japonicus)
    SONG Qiuzhen, YANG Yunjie, WAN Liwei, WU Hengye, ZHAO Chong
    Journal of Dalian Fisheries University. 2025, 40(3): 440-450. https://doi.org/10.16535/j.cnki.dlhyxb.2025-015
    Abstract (419) PDF (183) HTML (1)   Knowledge map   Save
    A good gut microbiota is of vital importance to digestion of sea cucumber (Apostichopus japonicus). This study used paraffin sectioning, behavioral observation, and 16S rRNA high-throughput sequencing techniques to investigate the effects of ingested glass microbeads on the behavior and intestinal structure of A. japonicus,andthensystematically elucidated the impacts of those microbeads on the composition of intestinal microbial community of sea cucumber. The results showed that no significant differences were observed in crawling and feeding behaviors or gut morphology among group Q (complete replacement of sea mud with microbeads), group I (partial replacement of sea mud withmicrobeads), and group O (control). The abundance and diversity of the gut microbiota of A. japonicus in group Q were significantly higher than those in group O (P<0.05), while no significant difference was observed in the relevant indicators in group I. However, the ingested glass microbeads showed minimal impact on the dominant microbial communities. Proteobacteria were the primary dominant phylum in all three groups, and Bacteroidota persisted as the second dominant phylum, suggesting that the original balance of microbial communities was not disrupted. Compared to group O, group Q exhibited specific bacterial phyla including NB1-j, Hydrogenedentes, and Desulfobacterota, along with specific bacterial families such as the PS1 clade, Hydrogenedensaceae, Hyphomicrobiaceae, and Arenicellaceae.But group I showed no differential bacterial phyla. This study demonstrates that complete substitution of sea mud with glass microbeads in the substrate enhances the richness and diversity of intestinal microbiota of A. japonicus, effectively enriching the beneficial microorganisms while maintaining normal behavioral activities, intestinal structural integrity, and intestinal microecological balance. This study provides theoretical insights for culture of A. japonicus.
  • A study on the ability of Brazilin to penetrate the blood-brain barrier of Nile tilapia (Oreochromis niloticus) in vitro
    ZHENG Mengjie, LI Zixin, CHEN Yanghui, CAI Jia, HUANG Yu, TANG Jufen, MOU Xidong, YANG Yexin, WANG Bei
    Journal of Dalian Fisheries University. 2025, 40(3): 372-381. https://doi.org/10.16535/j.cnki.dlhyxb.2024-237
    Abstract (416) PDF (317) HTML (2)   Knowledge map   Save
    To explore the ability of Brazilin to penetratethe blood-brain barrier and its potential to treat brain diseases in tilapia, a UPLC-MS/MS analytical method was established for the determination of Brazilin in an in vitro model of the blood-brain barrier of the Nile tilapia (Oreochromis niloticus). The antibacterial efficacy of 36 different Chinese herbal medicines against Streptococcus agalactiae (ZQ0910) was assessed by using the agar diffusion method, and the Minimum Inhibitory Concentration of these extracts was determined using a two-fold dilution technique. Herbs exhibiting significant antibacterial activity were selected for a subsequent in vitro permeability experiment to penetrate blood-brain barrier. The effects of an antibacterial agent on the proliferation of tilapia brain endothelial cells (TVEC-01) and tilapia astrocyte cells (TA-02) were determined using the CCK8 method in a co-culture model of TVEC-01 and TA-02 cells. The effects of bacteriostatic drugs on the expression levels of the tight junction-associated proteins JAM-B, ZO-1, and Claudin-5 in the in vitro blood-brain barrier were then detected using qRT-PCR. Finally, the concentrations of the bacteriostatic drugs in pooled samples from the in vitro blood-brain barrier model were determined using UPLC-MS/MS. The experimental results showed that of the 36 herbs tested in the experiment, the sappan wood aqueous extract had the greatest in vitro inhibitory effect on S. lactis. The results of CCK8 experiment showed that Brazilin in the range of experimental concentrations had no significant inhibitory effect on the growth of the TVEC-01 and TA-02 cells constituting the blood-brain barrier. Concentrations of 20, 40, and 80 μmol/L of Brazilin all regulated down the expression levels of tight junction proteins JAM-B, ZO-1, and Claudin-5 (P<0.05). Brazilin could be detected in samples from the lower pool of blood-brain barrier model after 30-minute administration, and the penetration concentration increased with increase in concentration of drug administered. These findings demonstrate that Brazilin can penetrate the blood-brain barrier and exert antibacterial effects, and provide a feasible strategy for screening drugs to treat brain diseases.
  • Comparative analysis between germplasm characteristics of cultured stocks of Parabramis pekinensis in the Yangtze River and in the Heilongjiang River Basin
    SHI Changhe, ZHENG Xianhu, SUN Zhipeng, LIU Tianqi, NA Rongbin, LU Cuiyun
    Journal of Dalian Fisheries University. 2025, 40(1): 146-155. https://doi.org/10.16535/j.cnki.dlhyxb.2024-129
    Abstract (416) PDF (119) HTML (0)   Knowledge map   Save
    To explore the difference in germplasm characteristics of Parabramis pekinensis in Yangtze River from that in Heilongjiang River,and improve the germplasm resources of this species in the Heilongjiang River,the external morphological characteristics,muscle nutrient compositions,and genetic structures of cultured stocks of P. pekinensis in the Yangtze River (WB) and the Heilongjiang River (FB) were analyzed.An analysis of the morphological data shows significant differences (P<0.05) between the WB and FB populations in the ratios of total length (TL),body height (BH),head length (HL),snout length (WL),eye diameter (ED),eye cross (EC),and caudal peduncle height (FH) compared with the standard length (SL).The body shape of fish species from Changjiang River is considerable different to that from Heilongjiang River and fish stocks from Changjiang River may be used as the hybrid breeding stocks of P. pekinensis.Muscle nutrient composition analysis shows that the crude lipid content (0.63 g/100 g) of the WB stock is significantly lower than that of the FB stock (1.57 g/100 g) (P<0.05),and that the difference in crude protein contents is not significant (P>0.05).The total amino acid,semi-essential amino acid,non-essential amino acid,and flavor-amino acid contents of the WB stock are significantly higher than those of the FB stock (P<0.05).But the saturated fatty acid,monounsaturated fatty acid,and polyunsaturated fatty acid contents of the WB stock are significantly lower than those of the FB stock (P<0.05).Analysis of the genetic structure based on genome wide SNP markers reveals that the polymorphic information contents (PIC) of the WB and FB stocks are 0.100 7 and 0.100 4,respectively,indicating low levels of genetic diversity (PIC<0.25).The genetic differentiation coefficient (Fst) between the stocks is 0.030 5,and the gene flow (Nm) iss 7.942 8,suggesting extensive gene exchange between the two stocks.This study provides a favorable reference for the development,breeding,and sustainable utilization of P. pekinensis.
  • Molecular characterization of estrogen receptor genes (esr2a, esr2b) of largemouth bass (Micropterus salmoides) and their mechanism of immunity action
    ZHU Zhilin, GAO Fengying, DONG Junjian, SUN Chengfei, ZHANG Hetong, LI Jiaxin, WANG Qingkui
    Journal of Dalian Fisheries University. 2025, 40(3): 417-430. https://doi.org/10.16535/j.cnki.dlhyxb.2024-246
    Abstract (401) PDF (200) HTML (1)   Knowledge map   Save
    Estrogen receptors play a crucial role in the reproductive processes of teleost fish. Recent studies have indicated that estrogen signaling pathways are related to immune responses of fish. To investigate the potential immune functions of the estrogen receptor genes 2a (esr2a) and 2b (esr2b) of the largemouth bass (Micropterus salmoides), cDNA fragments of the esr2a and esr2b genes were isolated and their sequence characteristics were analyzed. Expression patterns and temporal expression profiles after injection with Nocardia seriolae, Largemouth bass virus (LMBV), and polyinosinic-polycytidylic acid (Poly I：C), as well as treatment with the exogenous hormone 17β estradiol (E2) were explored.The cDNA sequence of the esr2a gene was 1 912 bp in length, with a 3′untranslated region (UTR) of 113 bp, a 5′UTR of 125 bp, and an open reading frame (ORF) of 1 674 bp, encoding a total of 557 amino acids. The cDNA sequence of the esr2b gene was 2 358 bp in length, with a 3′UTR of 293 bp, a 5′UTR of 52 bp, and an ORF of 2 013 bp, encoding a total of 670 amino acids.Both the esr2a and esr2b proteins in the largemouth bass were characterized as unstable, hydrophilic, and acidic. In addition, both esr2a and esr2b contained one ZnF_C4 domain and one HOLI domain. Real-time fluorescence quantitative PCR analysis showed that, in normal fish, the esr2a gene exhibited its highest expression in kidney, while the esr2b gene showed its highest levels in spleen and liver. Following injection with N. seriolae, significant decreases in the expression levels of the esr2a and esr2b genes were observed in the liver of largemouth bass (P<0.05). Conversely, after injection with LMBV, the expression levels of the esr2a and esr2b genes in the kidney increased significantly (P<0.05). In contrast, injection with Poly I：C significantly inhibited the expression of esr2a and esr2b in both the kidney and spleen (P<0.05). Additionally, E2 treatment inhibited the expression of the esr2a and esr2b mRNAs in the spleens of both male and female fish (P<0.05). This study suggests a potential association between the esr2a and esr2b genes and the immune response in largemouth bass.
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Editor-in-Chief: SONG Linsheng
Deputy Editor-in-Chief:
ZHANG Guochen
LI Zhijun
LIU Ying
ZHANG Dongdong (executive)
Sponsor: Dalian Ocean University
Supervisor: Education Department of Liaoning Province
Address: No. 52,Heishijiao street, Shahekou District, Dalian, Liaoning Province, China
Postal code: 116023
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E-mail：xuebao@dlou.edu.cn
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