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  • Applications and future prospects of DeepSeek and fishery intelligent agent in modern fisheries:a review
    MA Zhihong, LIU Ying
    Journal of Dalian Fisheries University. 2025, 40(2): 185-194. https://doi.org/10.16535/j.cnki.dlhyxb.2025-062
    Abstract (622) PDF (795) HTML (0)   Knowledge map   Save
    The rapid advancement of artificial intelligence (AI) has provided novel pathways for the intelligent transformation of the fisheries industry.This paper explores the application and development prospects of DeepSeek in fisheries.As a representative domestically developed open source large model,DeepSeek demonstrates high adaptability across multiple fisheries scenarios by leveraging its multimodal integration,dynamic reasoning,and knowledge graph construction capabilities.By constructing a DeepSeek-based fisheries intelligent agent,a new paradigm can be established for core production processes,including precision aquaculture management,breeding optimization,disease prevention,and resource stock assessment.Through interdisciplinary collaborative innovation,the co-construction of an “AI cooperative” ecosystem,technology scenario validation,and algorithm iteration,this study addresses critical challenges such as multisource data governance bottlenecks,industrial collaboration barriers,tacit knowledge conversion obstacles,and spatiotemporal decision making adaptation challenges.These efforts aim to achieve comprehensive integration of AI and fisheries practices,ultimately building a sustainable ecosystem for the intelligent evolution of the fisheries industry.
  • Research progress on application of deep learning in fish recognition, counting, and tracking: a review
    XU Wenjie, FANG Hui, YANG Shenglong, ZHANG Shengmao, SHI Yongchuang, WU Zuli, YU Shengchi, XIONG Xinquan, YANG Haodong, DAI Yang​
    Journal of Dalian Fisheries University. 2024, 39(5): 874-887. https://doi.org/10.16535/j.cnki.dlhyxb.2024-080
    Abstract (613) PDF (274) HTML (0)   Knowledge map   Save
    Fish recognition is essential for fisheries management and ecosystem protection measures due to provide a variety of information on the aquatic ecosystem, especially the abundance of fish resources and aquatic health. Time consuming, laborious and subjective manual fish recognition will lead to fish stress reaction after artificial contact, and is extremely unfavorable to the growth of fish. The traditional machine learning method avoids the adverse effects caused by contact with the fish directly, and still needs to manually extract the features and design the image vectorization method according to the recognition problem. However, deep learning known as end to end learning, different from the above recognition methods, is capable of obtaining high level features from the input data and mining the distribution patterns of the data, can free the labor, circumvent the tendency of subjective recognition, and minimize the stress reaction of fish, which is a positive contribution to the development of smart fisheries and precision aquaculture. The application of deep learning in fish recognition is described, and uniform recognition standard is proposed to clarify future research directions. Also, the level of applied research should be continuously improved to enhance practicability and intelligence. Addressing the interface compatibility between equipment and models is also recommended to increase flexibility for researchers in choosing tools, aiming to provide reference for researchers working on fish recognition using deep learning methods.
  • Research progress in the aquaculture biology of the Golden Pompano (Trachinotus ovatus)
    CHEN Huapu
    Journal of Dalian Fisheries University. 2025, 40(1): 1-11. https://doi.org/10.16535/j.cnki.dlhyxb.2024-155
    Abstract (610) PDF (235) HTML (0)   Knowledge map   Save
    The golden pompano(Trachinotus ovatus) is a popular fish variety for marine cage culture and large-scale offshore aquaculture facilities.Successive breakthroughs in key technologies for breeding and cultivating golden pompano have promoted the rapid development of its industrial aquaculture in China.However,issues such as the decline in farming performance,caused by relatively weak fundamental biological and genetic breeding research,have become bottlenecks for sustainable development of this industry.We describe the current state of research and development in the industry and review the biological characteristics of T. ovatus,including resource distribution,habitat,morphological traits,and reproductive biology.We also summarize recent research achievements in artificial breeding,aquaculture,physiological ecology,and genetics.In light of the development,conservation,and the future potential of golden pompano resource,we propose future research directions,including selective breeding,basic genetic mechanisms,gonadal development regulation,and new strain development,to provide references for the future stable development of golden pompano aquaculture sector.
  • Analysis of environment of Dafeng River in Beihai, Guangxi and health status of cultivated Hong Kong oyster (Crassostrea hongkongensis)
    LIU Ranyang, GAO Lei, ZHANG Xingzhi, YANG Wenwen, ZHAO Junyan, ZHAO Bao, YU Haijie, XU Jixiang, LIU Lu, PENG Jinxia, WANG Lingling, SONG Linsheng
    Journal of Dalian Fisheries University. 2024, 39(4): 551-558. https://doi.org/10.16535/j.cnki.dlhyxb.2023-275
    Abstract (573) PDF (743) HTML (0)   Knowledge map   Save
    To elucidate the impacts of water environment of Dafeng River in Beihai, Guangxi, on health status of Hong Kong oyster(Crassostrea hongkongensis) cultured therein, the physicochemical factors in water quality, planktonic microalgae, bacterial abundance, and energy reserve and oxidative stress of Hong Kong oysters were surveyed in the main Hong Kong oyster farming area of Beihai Sea of Guangzhou, the mouth of the Dafeng River and the upper waters(108°52′49″E, 21°38′26″N; 108°51′30″E, 21°46′22″N) in September and October 2023. The results showed that, the estuarine water temperature was 28.1 ℃, with a salinity of 20.89, total bacterial abundance of 7.22×108 cells/L and Vibrio abundance of 1.49×107 cells/L in September. Diatom abundance was found to be approximately 1.3×107 cells/L, with the dominant species of Skeletonema costatum. The malondialdehyde (MDA) content of 9.60 nmol/mg protein was observed in the gill of cultivated Hong Kong oysters, with glycogen content of 6.18 mg/g and glucose content of 2.59 μmol/g in the adductor muscle. In October,however, the estuarine water temperature was decreased to 25.7 ℃, accompanied by an increase in salinity to 25.50, with bacterial abundance decreased to 4.97×107 cells/L and Vibrio abundance to 1.40×104 cells/L and diatom abundance decreased to 4.3×105 cells/L in the water. MDA content was 1.02 nmol/mg protein in the gill of cultivated Hong Kong oyster, and glycogen and glucose content in the adductor muscle was decreased to 2.49 mg/g and 1.94 μmol/g, respectively. The water temperature and total bacterial abundance were similar to those in the estuarine waters in the upstream waters in October. However, salinity and Vibrio abundance were relatively low, with high diatom abundance. The glycogen and glucose contents in adductor muscle were increased in cultivated Hong Kong oysters. In summary, Hong Kong oysters cultured in the Dafeng River estuary faced multiple environmental stressors, including high temperature, elevated bacterial and Vibrio abundances in September, leading to increased oxidative stress. In October, these oysters primarily encountered a single environmental pressure, high salinity, and improved oxidative stress status. The decrease in algal food led to decline in glycogen content and resistance to environmental changes in October, and there were lower salinity, higher diatom abundance, and relatively healthier conditions for Hong Kong oyster cultivation in upstream waters in the Dafeng River.
  • Effects of temperature and salinity on the temporary rearing and stress preservation of grass carp (Ctenopharyngodon idellus)
    HE Jingyi, ZHENG WEI, HUANG Hui, CEN Jianwei, ZHAO Yongqiang, WANG Tian, WEI Ya, HAO Shuxian, YANG Shaoling, CHEN Chen
    Journal of Dalian Fisheries University. 2024, 39(4): 597-605. https://doi.org/10.16535/j.cnki.dlhyxb.2023-270
    Abstract (511) PDF (725) HTML (0)   Knowledge map   Save
    In order to explore the low-temperature preservation and transportation technology of freshwater fish, serum biochemical indices and contents antioxidant index were detected in grass carp (Ctenopharyngodon idellus) with body weight of (1 200±100) g at water temperature from 23-25 ℃ (control group) to 24, 20, 18, 16, 14, and 12 ℃ at cooling rate of 3 ℃/h at >25 ℃, 10 ℃ at ≤ 25 ℃, and 2 ℃/h at ≤ 10 ℃ and at salinity of 0.5-5 for 48 h and total ammonia nitrogen concentration in water was determined. The results showed that the grass carp had critical temperature of 6-8 ℃, and semi-dormant temperature of 16 ℃ at salinity of less than 3. The grass carp with delayed stress at 16 ℃ and lower concentration of ammonia and nitrogen in the water column had the maximal survival rate, lower serum levels of AST, ALT, COR and GLU at 48 h of preservation, whereas the maximal enzyme activities of SOD, CAT, and GSH-Px were found, with significantly lower content of MDA than that in other groups (P<0.05). Lower ammonia nitrogen concentrations in the water column, the maximal survival rate of grass carp, generally lower levels of the biochemical indicators in the serum of grass carp, higher activities of antioxidant enzymes and lower levels of MDA were observed at salinity from 1 to 2 for 48 h of preservation.The Pearson and Mantle tests revealed that the effects of temperature on COR, GLU and GSH-Px, and salinity on ALT, AST, COR, GLU and SOD were more significant. The water quality was improved and long-distance transport of grass carp with water was facilitated at water temperature of 16 ℃ and appropriate salinity. The key parameters of the transient conditions of grass carp in this experiment provide technical support for the live transport of bulk freshwater fishes and reference for the development of relevant transient and transport.
  • Research progress and prospect of fish swimming mechanism: a review
    SUN Wei, LIU Suping, WU Gang, ZHOU Yinxin, REN Xiaozhong
    Journal of Dalian Fisheries University. 2024, 39(4): 698-707. https://doi.org/10.16535/j.cnki.dlhyxb.2023-283
    Abstract (501) PDF (100) HTML (0)   Knowledge map   Save
    Fish as the oldest vertebrate have excellent underwater swimming ability after long-term natural evolution. Research of the swimming mechanism of fish including its movement characteristics, stress and interaction with water is not only helpful to deeply understand the evolution process of fish, but also provides scientific reference and innovative ideas for environmental protection, bionic design, agriculture and aquaculture. Fish have two modes of swimming: body/tail fin (BCF) and intermediate fin/opposing fin (MPF). The research progress of fish swimming mechanism are summarized from three aspects: theoretical research, experimental research and numerical research. In view of the existing problems in the research of fish swimming mechanism, it is proposed that further research should be carried out in the aspects of biological fish propulsion system, bionic fish application and multi-robot fish synergy in the future, so as to provide reference for marine fishery and marine industry development.
  • Growth characteristics and resource dynamics of rockfish (Sebastes schlegelii) in waters of Dachangshan Island
    CUI Jiuru, XUE Guojin, YIN Zengqiang, YANG Jisong, LI Guoxing, SUN Peiqi, TIAN Tao, CHENG Qian, LIU Yonghu
    Journal of Dalian Fisheries University. 2024, 39(4): 657-665. https://doi.org/10.16535/j.cnki.dlhyxb.2023-272
    Abstract (482) PDF (385) HTML (0)   Knowledge map   Save
    To understand the population dynamics of rockfish (Sebastes schlegelii) and determine the strategy of sustainable utilization,based on the surveyed data from 2011 to 2019 in Dachangshan Island waters,the relationship between body length and body weight was analyzed, the body length growth equation and body weight growth equation were established, and the resources of this species were evaluated in Dachangshan Island waters. The changes in eggs production per recruitment (EPR) and spawner biomass per recruition (SBR) of Sebastes schlegelii were simulatively analyzed under different management strategies. The results showed that the length-weight formula was described as W=4×10-5L2.934 4(R2=0.918 9,n=204). There were asymptotic body length(L∞)of 425 mm, the asymptotic body weight(W∞) of 2 064.5 g, growth curvature K of 0.23 a-1 and the theoretical initial age t0 of -0.58 a, and the inflection age of body weight growth of 4.1 a, with the total mortality(Z)of 1.34 a -1, the natural mortality(M)of 0.208 9 a-1 and the fishing mortality(F) of 1.311 1 a-1. The critical age was found to be 5.7 a, and the biomass to be 1 084.68 t. It would be more reasonable to set the closed fishing period from March to May, when EPR values return to 67.54% and SBR values to 85.35% during the unfished period. It is found that the resources of S. schlegelii are overfished, the population is seriously juvenile, and the spawner biomass is seriously insufficient in waters of Dachangshan Island, with urgent need of scientific management. The findings are expected to provide a reference for the rational use of population of S. schlegelii in Dachangshan Island.
  • Current status of development and application of unmanned vessel technology in fisheries: a review
    BA Yaoji, TANG Fenghua, ZHANG Shengmao, WANG Fei, DAI Yang, WU Zuli
    Journal of Dalian Fisheries University. 2024, 39(5): 888. https://doi.org/10.16535/j.cnki.dlhyxb.2024-156
    Abstract (475) PDF (187) HTML (0)   Knowledge map   Save
    With the development of artificial intelligence, automatic collision avoidance, and unmanned navigation technologies, unmanned vessels are being used more and more widely, especially in the fishery field, which plays an increasingly important role. The key technologies of unmanned vessels are introduced, mainly including environment sensing, path planning, target analysis and formation control technology, and main applications of unmanned vessels in the fishery field are analyzed. Unmanned vessel technology is also challenged by many problems including difficulty in release and recovery, poor environmental adaptability, limited longrange detection and high cost, although unmanned vessel technology shows great potential. Finally, the future development trend and research direction of unmanned vessels are discussed. With technological progress, unmanned vessels are expected to become an important tool for fishery resources investigation and management.
  • Fish diversity in important estuaries in Liaodong Peninsula based on environmental DNA metabarcoding
    CUI Xiaoyu, ZHANG Yunlei, ZHANG Jinyong, SUN Yi, WANG Yuan, LI Hongjun
    Journal of Dalian Fisheries University. 2024, 39(4): 578-588. https://doi.org/10.16535/j.cnki.dlhyxb.2023-278
    Abstract (431) PDF (533) HTML (0)   Knowledge map   Save
    In order to explore the composition and diversity distribution of fish in important estuaries in the Bohai Sea and the North Yellow Sea from molecular technology, fish community diversity was surveyed and investigated in 12 important estuaries in Bohai Sea and North Yellow Sea (121°18′～124°19′E, 39°10′～40°54′N) by environmental DNA metabarcoding technique. The results showed that 74 fish species were found in Bohai Sea, belonging to 2 classes, 19 orders, 41 families and 57 genera, with the maximal proportion of species in Perciformes (45%). A total of 76 fish species were detected in the Northern Yellow Sea, belonging to 2 classes, 19 orders, 42 families and 59 genera, with the maximal proportion of species in Perciformes (42%). There was the similar variation trend of diversity index in Bohai Sea to that in the Northern Yellow Sea, with lower diversity index in the Bohai Sea than that in the Northern Yellow Sea, indicating the rich fish diversity in the Northern Yellow Sea. NMDS analysis and ANOSIM test based on Bray-curtis distance showed that the difference between fish communities was larger in Bohai Sea and Northern Yellow Sea than the difference within the groups (P=0.001). Environmental DNA metabarcoding survey was found to be featured by significant advantages in sensitivity, non-destructiveness and cost-effectiveness, compared with traditional fish diversity survey methods, and thus suitable for fish diversity survey. The findings demonstrated that eDNA metabarcoding technology supplemented the results of fishery resources investigation and had a wide range of business application prospects in fish diversity investigation.
  • Research advances on application of computational fluid dynamics methods in bionic robotic fish:a review
    LIU Bilin, HUANG Xiaoshuang, CHEN Xinjun, ZHANG Ying, KONG Xianghong
    Journal of Dalian Fisheries University. 2024, 39(4): 719. https://doi.org/10.16535/j.cnki.dlhyxb.2023-294
    Abstract (383) PDF (1207) HTML (0)   Knowledge map   Save
    Computational fluid dynamics as an important numerical simulation method in reaearch of the hydrodynamic properties of bionic fish has been widely used in many fields such as bionics and oceanography. The type of literature, journal distribution, trend of publication volume, authors, research institutions, and highly cited literature were analyzed in 146 English literature and 201 Chinese literature retrieved using CNKI Chinese database and Web of Science core collection database, and bibliometric analysis based on Cite Space software by combining with keyword network knowledge mapping and keyword clustering mapping to explore the research hotspots and future development trends of computational fluid dynamics in the field of bionic machine fish. The results of the analysis showed that the number of Chinese and foreign literature in the field of bionic fish was increased year by year, with high impact factor and citation frequency of bionic journals and literature. The research disciplines involved in many interdisciplinary disciplines including engineering, robotics, mechanics and material science, with the hot spots and key directions of the dynamics model, 3D flow simulation, design and fabrication. To address the current limitations in research, future investigations are suggested to focus on rigorous exploration of aquatic swarm behavior simulation, the mechanisms behind fish lateral line sensation, and hydrodynamic testing of bionic robotic fish. Such comprehensive research is essential for promoting interdisciplinary collaboration and offering a solid scientific foundation to overcome obstacles and chart a clear path forward in the development of robotic fish technologies.
  • Functional analysis of CgPOU3F4L in the apoptosis regulation of Pacific oyster (Crassostrea gigas) exposed to high-temperature stress
    LI Qingsong, GAO Lei, HU Li, YE Jiayu, WANG Lingling, SONG Linsheng
    Journal of Dalian Fisheries University. 2024, 39(6): 905-914. https://doi.org/10.16535/j.cnki.dlhyxb.2024-047
    Abstract (380) PDF (309) HTML (0)   Knowledge map   Save
    In order to probe the role of CgPOU3F4L gene in the response to high-temperature stress in Pacific oyster (Crassostrea gigas), the composition and evolutionary features of POU family genes and the involvement of CgPOU3F4L gene in the regulation of haemocyte apoptosis were investigated in 2 year old Pacific oyster exposed to water temperature of 28 ℃ for 72 h. The results revealed that the Pacific oyster genome contained four POU family genes, namely CgPOU2F1, CgPOU3F4, CgPOU3F4L, and CgPOU4F3. The CgPOU3F4L possessed a unique amino acid sequence, the unclear functional differentiation characteristics of which are not yet clear. There were significantly higher mRNA expression levels of CgPOU3F4L in mantle, adductor muscle and haemocyte than that in hepatopancreas, as high 91.32, 19.24 and 7.92 fold as that in hepatopancreas (P<0.05), as high 2.63 fold at 12 h, 3.05 fold at 24 h and 3.62 fold at 36 h in the Pacific oyster exposed tp high-temperature stress as that in the control group (15 ℃) (P<0.05). POU transcription binding sites were found at 1 197-1 207 and 1 286-1 297 bp in the promoter region of apoptosis-related genes CgBcl-2 and CgCaspase3, respectively. RNAi method was applied to inhibit the expression of CgPOU3F4L, and the mRNA expression of CgBcl-2 in CgPOU3F4L-RNAi group was shown to be increased significantly, which was 7.49-fold of that in the control group (P<0.001). Conversely, the mRNA expression level of CgCaspase3 was found to be decreased significantly, which was 0.57-fold of that in the control group (P<0.05). Additionally, the apoptosis rate of haemocytes (16.53%) was significantly lower than that in the control group (45.90%, P<0.001). It is approved that CgPOU3F4L gene regulates the apoptosis of haemocytes through the transcription regulation of CgBcl-2 and CgCaspase3 after high-temperature stress. These findings provide theoretical basis and reference for further understanding of the evolutionary characteristics of POU family and the mechanism of environmental stress response in oyster.
  • Research progress on wild training for breeding and releasing fish: a review
    CHEN Shihong, TIAN Tao, YU Xiaoming, WU Zhongxin, YIN Zengqiang
    Journal of Dalian Fisheries University. 2024, 39(4): 684-697. https://doi.org/10.16535/j.cnki.dlhyxb.2023-255
    Abstract (372) PDF (874) HTML (0)   Knowledge map   Save
    The increase in fishery resources via artificial propagation and releasing is a crucial method for replenishing biological resources and ensuring the sustainable development of fisheries, and developed rapidly in China in past decades. However, the high mortality of artificially bred seedlings after release led to the failure in most artificial proliferation and releasing of fish without the expected results, thereby affecting the benefits of fishery resource proliferation. One of the effective methods to improve the quality and survival rate of released seedlings is to conduct wild training through various methods before release, which plays an important role in enhancing the overall benefits of proliferation and release. The current situation of the proliferation and release of fishery resources in China was reviewed, focusing on scientific issues of concern in the research of proliferation and release. The reasons for the low survival rate of fish after release and the research progress of fish wild training were evaluated from several aspectsincluding food cognition, predator information, environmental complexity, and fish swimming ability. It is suggested to carry out genetic improvement, test sperm and egg quality, count injury rates,mortality rates and practice of analyzing blood biochemical indicators, controlling feeding space, improving the complexity of aquaculture environment, implanting chips and biological tracking for fish wild training, improving the quality of life of breeding and releasing species in the future, in order to provide reference basis for promoting water ecological restoration and sustainable development of the fisheries.
  • Pathogenicity and drug resistance of Bacillus paranthracis strain BC006 from bluntnose black bream (Megalobrama amblycephala) based on whole genome analysis
    LIU Zhenhong, LI Wenhui, LI Luying, GAO Meng, YANG Zhijun, LI Yan, PENG Kaisong
    Journal of Dalian Fisheries University. 2024, 39(5): 754-765. https://doi.org/10.16535/j.cnki.dlhyxb.2024-033
    Abstract (349) PDF (61) HTML (0)   Knowledge map   Save
    In order to understand the pathogenicity and drug resistance of Bacillus paranthracis from a genetic perspective, whole genome sequencing, in vivo bacterial virulence testing, and antibiotic sensitivity testing were conducted in the pathogenic bacterium B. paranthracis designated as strain BC006 isolated from viscera of diseased bluntnose black bream (Megalobrama amblycephala). The results showed that, the genome of B.paranthracis strain BC006 consisted of 5 457 931 bp, including 1 chromosome (GenBank ID CP119629) and 7 plasmids (GenBank ID CP119630～CP119636), containing 5 539 coding genes,with the maximal degree of collinearity with B. paranthracis strain 1710 from bovine. The LD50 induced by intraperitoneal injection of BC006 was found to be 4.68×105 CFU/g in bluntnose black bream and 4.22×105 CFU/g in mice. The B. paranthracis strain BC006 carried 43 virulence genes, including alo encoding the hemolysin I component, hly Ⅲ encoding the hemolysin Ⅲ component, nheA/B/C encoding the non hemolytic enterotoxin (Nhe), and sph encoding sphingomyelinase (SMase), whose virulence genes were associated with β- hemolysis and enteritis by BC006. BC006 carried 16 resistance genes. Tet (45) and tetB (P) were shown to be involved in the resistance to tetracycline and doxycycline, and MPhL was involved in the resistance to erythromycin. Both Bc Ⅰ and Bc Ⅱ were involved in the resistance to cefotaxime and penicillin G. QacJ was involved in the resistance to metronidazole, trimethoprim, rifampicin, norfloxacin, enrofloxacin, ciprofloxacin, ofloxacin, and compound sulfamethoxazole. The findings indicate that Bacillus subtilis BC006 is a multidrug-resistant bacterium carrying multiple virulence factors, and a newly discovered pathogenic bacterium to bluntnose black bream. It is recommended to use fluophenicol and neomycin sulfate sensitive to this bacterium in national standard fishing medicines.
  • Effects of cold exposure on the immune indicators and gene expression of Ruditapes philippinarum
    CHEN Sunan, KONG Xue, SONG Manzong, CAI Yuefeng, ZHI Ying, SHEN Xin
    Journal of Dalian Fisheries University. 2025, 40(1): 12-24. https://doi.org/10.16535/j.cnki.dlhyxb.2024-121
    Abstract (344) PDF (223) HTML (0)   Knowledge map   Save
    The Manila clam,Ruditapes philippinarum,is an economically important bivalve shellfish whose survival is affected by a variety of environmental factors,temperature being a key factor affecting its normal activities.Much attention has been given to the response of shellfish to high temperature,while fewer studies have been conducted on their response to low temperature.Considering the increasing occurrence of extreme weather,such as extreme cold,research is required on the adaptation of shellfish to low temperatures.In this study,R. philippinarum was exposed to low temperature for 60 days.Its immune indicators were systematically measured and analyzed,and the differentially expressed genes (DEGs) obtained were analyzed by using the GO/KEGG enrichment databases.A total of 8 344 and 2 674 DEGs were found in the gill tissues of R. philippinarum after 20 and 60 days of cold exposure,respectively.Enrichment analysis of these DEGs revealed that R. philippinarum has potential low temperature coping strategies in biological processes such as protein processing,substance metabolism,antioxidant,immune and anti-apoptosis.Regarding protein processing,the expression of the SAR1B,HSP20,HSP70,TRX,and SEC61G genes were up-regulated during protein processing,suggesting that R. philippinarum may maintain cellular stability at low temperatures by enhancing protein synthesis.Phosphatidylcholine (PC) is a key component of cell membranes,and the up-regulated expression of PC synthesis-related genes in this study suggests that R. philippinarum may maintain cell membrane stability under cold exposure by enhancing PC synthesis.In this study,the expression of the SOD,CYP450,GST,and GPX genes were up-regulated,and the MDA content decreased when the SOD activity increased during the late stage of cold exposure,suggesting that hypothermia induces SOD to scavenge excessive ROS in the organism,thus protecting it from hypothermic injury.In addition,the immune and anti-apoptosis related genes were also significantly activated at low temperature.These results provide basic data for furtherstudy on the mechanism of low-temperature tolerance in R. philippinarum and provide theoretical support for the selection and breeding of low-temperature tolerant shellfish varieties.
  • Analysis of effect of morphological traits on body weight of male and female pear-shaped ringed edge snail Bellamya purificata
    WU Jiaqian, ZHOU Kangqi, ZHANG Caiqun, WEI Xiaokai, YE Hua, PENG Jinxia, LIN Yong, QIN Junqi, CHEN Zhong, LUO Hui, WEI Pinyuan, HE Pingping, PAN Xianhui
    Journal of Dalian Fisheries University. 2024, 39(4): 622-633. https://doi.org/10.16535/j.cnki.dlhyxb.2023-254
    Abstract (337) PDF (337) HTML (0)   Knowledge map   Save
    To investigate the correlation between morphological traits and body weight in male and female pear-shaped ringed edge snail Bellamya purificata, 11 traits including body weight, shell width and shell height were measured in 1007 snail samples collected from Guangxi Nanfan Aquatic Breeding Base after sex identification and the correlation among the morphological traits was analyzed by trail analysis and multiple regression analysis. The results showed that there was significant correlation between each morphological trait and each quality trait (P<0.05). The body weight of both male and female snails was mainly affected by shell height, with a flux coefficient of 0.927. The soft body weight was primarily affected by shell height (0.904) in male snail, and by shell width (0.899) in females. Muscle weight was found to be largely influenced by the height of the body snail layer (0.899) in male snails, and by the combined effect of shell width, aperture width, body whorl height, and the spiral height in females. The maximal individual coefficient of determination for body weight of female (0.489) and male (0.281) and soft body weight of male (0.561) was observed in shell height. There were the maximal individual coefficient of determination for soft weight (0.190) and muscle weight (0.080) in female snails in shell width. In summmary, the body weight, soft weight, and muscle weight of male and female snails were shown to have good potential for selection. Shell height should be selected as a key auxiliary selection trait when selecting for higher body weight and soft weight. When muscle weight is used as a selection target, body snail layer height should be used as the main selection trait in male snails, while the effects of shell width, aperture width, and body whorl height should be considered in female snails. In this study, a better equation was established by stepwise multiple regression for body weight, soft body weight, and muscle weight of male and female snails, which provides an important reference basis for the subsequent selection of good pear-shaped ringed edge snail.
  • Isolation and application of a bacterial strain with potential probiotic effects from intestinal tract of Manila clam (Ruditapes philippinarum)
    XIE Xiaochen, WANG Bo, JIANG Jialin, ZHAO Xinyan, LI Jiaxi, QIU Xianbo, HUO Zhongming, FANG Lei
    Journal of Dalian Fisheries University. 2024, 39(4): 568-577. https://doi.org/10.16535/j.cnki.dlhyxb.2023-259
    Abstract (332) PDF (336) HTML (0)   Knowledge map   Save
    With an attempt to screen bacteria with potential probiotic effects on Manila clam (Ruditapes philippinarum), culturable bacteria were first isolated from the intestinal tract of the healthy clam and then subject to enzyme-producing ability examination by in vitro plate experiment. Those enzyme-producing strains were, morphologically observed and molecularly identified by plate counting method and the standard curve between the absorbance value and the bacterial cell number. The bacterium was examined for acid and bile salt tolerance, and the safety of the enzyme-producing bacterium on R.philippinarum larvae by challenging test. The Manila clam juveniles were reared to examine growth promoting effects. The results showed that a bacterial strain isolated from the intestinal tract of healthy clams designated as C26 produced amylase, protease and lipase was gram-negative and belonged to the genus of Pseudoalteromonas, with tolerabce to pH value of higher than 4.5 and a bile salts concentration ranging from 0 g/100 mL to 0.1 g/100 mL. In addition, Strain C26 had no pathogenicity to R.philippinarum and significantly improved the weight gain rate of the juveniles (P<0.05). To sum up, a safe Pseudoalteromonas strain C26 was screened and had probiotic effects on Manila clam larvae, which provides foundation for enrichment of the Manila clam probiotic strain resources.
  • Differential expression analysis of plasma proteomics in Nile tilapia (GIFT) before and after infection with Streptococcus agalactiae
    ZHANG Meiyan, ZHU Weijuan, LIU Zhigang, WANG Zhang, CAO Jianmeng, LU Maixin, KE Xiaoli
    Journal of Dalian Fisheries University. 2024, 39(4): 559-567. https://doi.org/10.16535/j.cnki.dlhyxb.2023-288
    Abstract (329) PDF (453) HTML (0)   Knowledge map   Save
    In order to probe the differentially expressed proteins (DEPs) of plasma and their associated signaling pathways from Nile tilapia (GIFT) Oreochromis niloticus before and after infection with Streptococcus agalactiae, the plasma DEPs were analyzed in the plasma collected from the tilapia with body weight of (150 g±10 g) intraperitoneally injected with 100 μL of S.agalactiae WC1535 suspension at concentration of 5×107 CFU/mL in the experimental group and 100 μL sterile PBS in the control group in via proteomics after 6 h of challenge via proteomics. Furthermore, GO and KEGG enrichment was performed for the DEPs. The results showed that 751 proteins were identified in the tilapia plasma, with 34 significantly different proteins including 9 proteins significantly up-regulated and 25 proteins significantly down-regulated. GO functional annotation showed that these DEPs were mainly involved in binding, motility and catalysis functions. KEGG pathway enrichment analysis revealed that the DEPs were significantly enriched in regulation of actin cytoskeleton, MAPK signaling pathway, central carbon metabolism in cancer, and proteoglycans in cancer. It was found that the DEPs from the tilapia infected with S.agalactiae were mainly enriched in the pathways related to energy metabolism, cellular motility and immune regulation. The findings provided basic data for further research on the molecular mechanisms of S.agalactiae infection in tilapia.
  • Expression of GDH gene and regulation mechanism of the GDH gene function by HIF-1α gene in sea urchin (Strongylocentrotus intermedius)
    NING Bingyu, WANG Haolin, SUN Jingxian, ZHAO Tanjun, CHANG Yaqing, ZHAN Yaoyao
    Journal of Dalian Fisheries University. 2024, 39(6): 926-937. https://doi.org/10.16535/j.cnki.dlhyxb.2024-057
    Abstract (322) PDF (251) HTML (0)   Knowledge map   Save
    In order to elucidate the molecular mechanism of GDH gene (glutamate dehydrogenase, GDH) expression and function regulated by the transcription factor HIF-1α gene in sea urchins (Strongylocentrotus intermedius), the promoter sequence of GDH gene and its structural characteristics were cloned and analyze by genome walking, luciferase reporter assay and RNA interference techniques and the binding site and regulatory relationship between HIF-1α gene and the promoter region of GDH gene were verified in S. intermedius. The effect of HIF-1α gene regulation on the expression of GDH gene on the energy production of coelomocytes and gonads of S. intermedius was investigated. The results showed that the total length of GDH gene promoter was found to be 1 067 bp, containing 12 promoter elements, 14 different transcription factor binding sites and 1 CpG island, with core promoter region ranging from -984 to -363 bp. There were two binding sites between HIF-1α gene and GDH gene promoter region (-683 to -649 bp; -280 to -264 bp), with a positive regulatory relationship between the two. The relative expression of GDH (mRNA and protein levels) and the activity of relative total GDH in coelomocytes and gonads were shown to be a downward trend, accompanied by a significant decrease in the relative content of ATP in S. intermedius exposed to inhibition of HIF-1α gene expression. These findings suggest that HIF-1α gene in sea urchin can affect the expression of GDH gene by positively regulating its transcriptional activity, and ultimately affect the ATP level in different tissues of the sea urchin.
  • Preparation and removal effect on ammonia nitrogen and phosphates in seawater and freshwater in kaolinite-oyster shell powder modified material
    YANG Yuxiang, SONG Kexin, YANG Guang, MA Xiaona, XIE Siqi, LIU Zishuo, FENG Zhihua
    Journal of Dalian Fisheries University. 2024, 39(4): 634-647. https://doi.org/10.16535/j.cnki.dlhyxb.2023-264
    Abstract (318) PDF (159) HTML (0)   Knowledge map   Save
    In order to adsorption removal of phosphate and ammonia nitrogen in water, oyster shell powder and kaolin were mixed in the ratio of 3∶1, and the modified oyster shell powder with the best adsorption effect was prepared and screened out under different conditions of MgO addition of 0%, 2%, 4%, 8%, and 12%; temperatures of 0, 600, 700, 800, 900, and 1 000 ℃ by scanning electron microscopy, and Fourier infrared spectrometry. The characteristics of the modified oyster shell powder was analyzed by scanning electron microscope, Fourier infrared spectrometer, specific surface and porosity analyser and X-ray diffractometer. The adsorption process was fitted by kinetic model and isothermal adsorption model, and the effects of initial pH and adsorption column on the adsorption of phosphate and ammonia nitrogen by modified oyster shell powder were analyzed. The results showed that the best adsorption performance of modified oyster shell powder was observed at 8% MgO addition and 800 ℃ calcination temperature, with the phosphate removal rates of 91.7% (seawater) and 82.25% (freshwater), and the ammonia nitrogen removal rates of 36.67% (seawater)and 47.71%(freshwater). The adsorption of phosphate by modified oyster shell powder conformed to the quasi-secondary kinetic model and the Freundlich model, and the maximum The adsorption of phosphate by modified oyster shell powder conformed to the quasi-secondary kinetic model and Freundlich model, with the maximum adsorption of 108.37 (seawater) and 101.99 (freshwater) mg/g, and the adsorption of ammonia nitrogen conformed to the Langmuir model, with the maximum adsorption of ammonia nitrogen of 0.27 (seawater) and 0.38 (freshwater) mg/g. The adsorption of ammonia nitrogen and phosphate by the modified oyster shell powder in the adsorption columns was facilitated by the acidic conditions, and the adsorption of ammonia nitrogen was more favorable by the neutral conditions. The adsorption amounts of modified oyster shell powder on ammonia nitrogen and phosphate were found to be 0.12, 78.95 mg/g (seawater) and 0.17, 70.34 mg/g (freshwater) in the adsorption column. In the application in the sewage into the sea, the effluent reached the standards of a class of water with the removal rate of 96.83% for phosphate, and ammonia nitrogen for 9.7%. The findings indicate that the low cost modified oyster shell powder has the potential to be applied to remove phosphate and ammonia nitrogen from water efficiently.
  • Effect of low temperature silencing and resuscitation on gene transcription and expression in sperm of Glyptocidaris crenularis
    WANG Hao, WANG Yongjie, TIAN Fenglin, RUAN Shuchao, TIAN Ye, LI Shufeng, DING Beichen, DING Jun, HAN Bing
    Journal of Dalian Fisheries University. 2025, 40(2): 195-203. https://doi.org/10.16535/j.cnki.dlhyxb.2023-244
    Abstract (315) PDF (128) HTML (0)   Knowledge map   Save
    To explore the regulatory mechanism of sperm motility in the yellow sea urchin (Glyptocidaris crenularis) during cryopreservation and resuscitation,RNA-Seq was used to analyze the gene transcription level of sperm after cryopreservation and seawater resuscitation.The results showed that 5.92-6.99 Gb of data were obtained for every group of sea urchin by high throughput sequencing,and that 113 728 transcripts and 73 814 Unigenes were assembled.And 15 333 Unigene annotations were made to the Kyoto Encyclopedia of Genes and Genomes database and classified into 290 pathways.The results of differentially expressed gene enrichment showed that low temperature treatment inhibited cell gap connection communication,oxidative phosphorylation and other pathways; and enhanced carbohydrate catabolism,pentose phosphate signaling,NADPH regeneration,glucose 6 phosphate metabolism,and other pathways after seawater resuscitation.The expression levels of differentially expressed genes Spe 8,Spe 12,CatSper2,and NKAa1,which are related to the regulation of sperm motility,were verified by fluorescence quantitative PCR.Their expression levels were significantly downregulated after low temperature silencing but significantly increased after seawater resuscitation.These results provide a theoretical basis and new clues for further research on the effects of cryopreservation and resuscitation on the regulatory mechanism of yellow sea urchin sperm.
  • Enrichment and metabolism of sulfamethazine in rockfish (Sebastes schlegelii)
    SUN Yiming, LIU Siyu, YONG Yanhua, LIU Yang, ZHAO Hongxia, CHEN Siyu, QU Baocheng
    Journal of Dalian Fisheries University. 2024, 39(5): 766-772. https://doi.org/10.16535/j.cnki.dlhyxb.2024-034
    Abstract (315) PDF (188) HTML (0)   Knowledge map   Save
    In order to evaluate the transport and transformation of antibiotics in marine fish and evaluate the ecological risk of antibiotics, the bioconcentration pattern and metabolic pathway of sulfamethazine(SMZ) were detected in rockfish Sebastes schlegelii reared in a 30 L container and exposed to 0(control group), 10, and 100 μg/L SMZ at water temperature of (20±0.5)℃ by liquid chromatography tandem mass spectrometry quantitative analysis and high-resolution mass spectrometry detection and analysis. The results showed that the maximal enrichment concentration of SMZ was observed in the fish exposed to SMZ in the third day during the whole exposure period, without significant difference between the maximum concentration of SMZ and the high and low concentrations of SMZ in fish. During the optimal enrichment period, the enrichment trend of SMZ in the fish was described as gill >muscle >liver. Metabolic analysis showed that the metabolites of SMZ in the test fish were N4-acetylated SMZ and 2-amino-4,6-dimethylpyrimidine. The main metabolic pathways were SMZ acetylation addition and N-S bond cleavage linked to 2-amino-4,6-dimethylpyrimidine. In conclusion, sulfonamides antibiotics can migrate to the main tissues and organs of marine fish, and the main metabolic pathways of SMZ in Sebastes schlegelii are acetylation addition and N-S bond breakage.
  • Effects of dietary Chinese herbal medicine Scutellaria baicalensis Georgi on growth performance, serum and liver biochemical indices and liver histology of channel catfish(Ictalurus punctatus)
    LIU Haonan, LENG Xiangjun, YOU Yuhai, LIU Jiawei, CHEN Yunfeng, LI Xiaoqin
    Journal of Dalian Fisheries University. 2024, 39(5): 739-745. https://doi.org/10.16535/j.cnki.dlhyxb.2024-026
    Abstract (306) PDF (172) HTML (0)   Knowledge map   Save
    In order to investigate the effects of dietary Chinese herbal medicine (Scutellaria baicalensis Georgi) on growth performance, antioxidant and non-specific immunity capacity of channel catfish Ictalurus punctatus, juvenile channel catfish with body weight of (4.68±0.02) g were reared in net cages of each 1.0 m× 1.0 m× 1.2 m and fed isonitrogen and isolipid basic diet containing 0 (control group), 5, 10 and 15 g/kg S. baicalensis for 60 days with 3 replicates per group and 30 individuals per replicate. Then the growth performance, serum non-specific immune capacity and antioxidant capacity in liver were determined, and the histological structure was observed in the liver of the channel catfish. The results showed that there were increase in final body weight, weight gain rate and specific growth rate in 5 g/kg and 10 g/kg Chinese herbal medicine groups, without significant difference (P>0.05) compared with those in the control group. The food conversion ratio, however, was found to be significantly decreased by the supplementation of 10 g/kg Chinese herbal medicine (P<0.05). The supplementation of 5 g/kg and 10 g/kg Chinese herbal medicine led to significantly increase the levels of lysozyme acid phosphatase, total protein in serum and the levels of total antioxidant capacity, catalase, and superoxide dismutase in liver (P<0.05), and to decrease the levels of alanine aminotransferase, aspartate aminotransferase, and total cholesterol content in serum and malondialdehyde content in liver (P<0.05), with low vacuolation rate of liver. The findings indicate that the supplementation of S. baicalensis in diets can improve the feed utilization efficiency and enhance the antioxidant activity of channel catfish, with the appropriate supplemental amount of S.baicalensis of 10 g/kg under the experimental conditions.
  • Comparison of high temperature tolerance in three population diallel hybrid population of red swamp crayfish (Procambarus clarkii)
    WU Weijie, XU Qizhou, WAN Tong, JIA Liang, CHENG Xi, LI Dianzhong, BAI Zhiyi
    Journal of Dalian Fisheries University. 2024, 39(4): 606-612. https://doi.org/10.16535/j.cnki.dlhyxb.2023-281
    Abstract (298) PDF (290) HTML (0)   Knowledge map   Save
    In order to establish a hybrid population of red swamp crayfish (Procambarus clarkii) with high temperature tolerance, nine F1 populations of complete diallel hybridization from three populations of red swamp crayfish,Wuhu, Jianhu, and Gehu, were used as broodstocks to obtain F2 populations through self pollination [Gehu♀×Jianhu♂(GJ), Jianhu♀×Gehu♂(JG), Gehu♀×Wuhu♂(GW), Wuhu♀×Gehu♂(WG), Jianhu♀×Wuhu♂(JW), Wuhu♀×Jianhu♂(WJ), Gehu♀×Gehu♂(GG), Jianhu♀×Jianhu♂(JJ), and Wuhu♀×Wuhu♂(WW)]exposed to the water temperature from 30 ℃ to 35 ℃ at raising rate of 1 ℃/2 h,and at raising rate of 0.5 ℃/4 h until the crayfish death. The tolerance of 9 F2 populations to high temperature was compared using evaluation indicators of upper thermal tolerance (UTT) and Arrhenius Break Temperatures (ABT) of heart rate, combined with Critical Thermal Methodology (CTM), median lethal Time (LT50) and other methods, and the correlation was analyzed between ABT values and traits including body weight. The results showed that the descending order of the UTT values in each group were ranked as GW>GJ>WJ>JG>GG>WW>WG>JW>JJ, with significantly higher in the GW group than that in the other groups (P<0.05). The ABT was expressed as GW>WJ>WW>WG>JW>JJ>JG>GJ>GG in each group, significantly higher in the GW group than that in the other groups (P<0.05). The CTMax was found to be GW>WJ>GJ>GG>WW>JG>JW>WG>JJ in different groups, significantly higher in the GW, WJ, and GJ groups than that in the other groups (P<0.05). The LT50 was described as GW>GJ>WJ>JG>WW>GG>JW=JJ>WG in the red swamp crayfish in each group. There was significant difference in heat tolerance (UTT) among individuals in the crayfish with different developmental stages(P<0.05), significantly higher heat tolerance in crayfish juveniles than that in remarkable crayfish just reached the market specifications (P<0.05). Before and after heat stress, there was a negative correlation between heart rate and body weight in crayfish, with correlation coefficients of -0.21 and -0.15, respectively (P<0.05). The (GW) population had superior to other populations in four high temperature tolerance indicators. Compared with UTT and other indicators, using ABT indicators to measure was less harmful to individuals and more suitable for breeding high-quality varieties.
  • Effect of trpa1b knockdown on high temperature tolerance in zebrafish(Danio rerio)
    GONG Xiaoting, YAN Qianqian, CHEN Liangbiao
    Journal of Dalian Fisheries University. 2024, 39(6): 948-955. https://doi.org/10.16535/j.cnki.dlhyxb.2024-046
    Abstract (297) PDF (293) HTML (0)   Knowledge map   Save
    To explore transient receptor potential ankyrin 1b (trpa1b) gene on zebrafish(Danio rerio) under high temperature conditions, zebrafish model with trpa1b gene deletion was constructed by CRISPR/Cas9 gene knockout technology. Survival, tissue apoptosis and other related gene expression changes were investigated in Trpa1b knockout (trpa1b-/-) zebrafish and wild-type (WT) zebrafish exposed to water temperature from 28 °C to 40 °C at a warming rate of 1 °C/h per hour by histological and transcriptome methods. Zebrafish tissues of different genotypes were sampled at 40 ℃. It was found that the overall survival time of trpa1b-/-homozygotes was shorter under high temperature. TUNEL staining showed that there was significantly higher apoptosis in gill and brain of trpa1b -/- homozygotes than that in wild-type zebrafish(P<0.05). Transcriptome sequencing analysis for gill tissues of different genotypes under normal temperature (28 ℃) and high temperature (38 ℃ and 40 ℃) revealed that the expression level of hspa1b and hsp90aa1.1 in trpa1b-/- homozygotes was significantly down regulated under high temperature (P<0.001), and that the expression level of intracellular calcium ions, ubiquitin protein transferase, lysosome and ribosome related genes were changed, which impaired their viability under heat stimulation. The findings indicate that Trpa1b deletion will lead to more obvious high-temperature intolerance in zebrafish due to the fact that trpa1b gene is an important gene required to maintain high temperature tolerance in zebrafish, which provides reference for further research on the mechanism of high temperature tolerance in fish.
  • Identification of the Duox gene in Exopalaemon carinicauda and preliminary study on its immune function
    LIU Dexue, FAN Menghao, CHENG Weitao, CAO Yanrong, HAN Wanyu, HU Guangwei
    Journal of Dalian Fisheries University. 2025, 40(1): 34-43. https://doi.org/10.16535/j.cnki.dlhyxb.2024-157
    Abstract (293) PDF (255) HTML (0)   Knowledge map   Save
    Dual oxidase (Duox) is a member of the nicotinamide adenine dinucleotide (NADPH) oxidase (NOX) family,which plays a crucial role in various biological processes,such as the production of reactive oxygen species,regulation of the biological clock,and the immune response.To explore the immune function of the Duox gene in Exopalaemon carinicauda,the cDNA sequence of the Duox gene was successfully cloned from E. carinicauda,and the amino acid sequence which it encoded was analyzed.Real-time fluorescence quantitative PCR technology (qRT-PCR) was used to analyze the tissue expression characteristics of gene and its expression changes infected with Vibrio parahaemolyticus.Finally,the mortality rate of E. carinicauda infected with V. parahaemolyticus after Duox gene knockdown was analyzed by using the RNA interference technique.The result shows that the coding region sequence length of the Duox gene in E. carinicauda is 4 518 bp,encoding 1 505 amino acids,reflecting typical structural characteristics of the dual oxidase.The Duox gene is expressed in all of the E. carinicauda tissues tested,with the highest expression level in gill tissues.After infected with V. parahaemolyticus,the Duox gene expression level in gills increases significantly (P<0.05).Infection with V. parahaemolyticus after injection of siRNA reagent significantly reduces the survival rate of E. carinicauda in the RNA interference group compared with the control group (P<0.05).This study shows that the Duox gene plays a role in the immune response of E. carinicauda against pathogenic infection,and provides an favorable reference for further exploration into the immune function and mechanism of Duox in E. carinicauda.
  • Structure and expression characteristics of taurine transporter CgTauT genes in Crassostrea gigas
    JIANG Chunyu, KONG Ning, SUN Shiqing, LI Xiang, LUO Cong, QIAO Xin, WANG Lingling, SONG Linsheng
    Journal of Dalian Fisheries University. 2025, 40(1): 25-33. https://doi.org/10.16535/j.cnki.dlhyxb.2024-125
    Abstract (290) PDF (180) HTML (0)   Knowledge map   Save
    To investigate the composition and expression characteristics of the taurine transporter (CgTauT) gene family in Crassostrea gigas,a genome-wide screening strategy was used to identify taurine transporter genes,and the qRT-PCR was used to analyse their tissue distribution pattern and to expression characteristics after taurine treatment.The results revealed that C. gigas contains four CgTauT genes,with each containing an SNF structural domain and 12 transmembrane domains.The four CgTauT genes were constitutively expressed in the various tissues of C. gigas,with CgTauT1 showing the highest expression in the adductor muscle,and CgTauT2,CgTauT3 and CgTauT4 showing the highest expression in the mantle.There were no significant correlations between the expression levels of the four CgTauT genes and the taurine contents of the tissues.Under taurine immersion treatment,the four CgTauT genes exhibited different temporal expression profiles in the mantle of C. gigas.The expression level of CgTauT1 increased after three hours of treatment.With prolonged taurine treatment,the mRNA expression level of CgTauT3 increased gradually,while that of CgTauT4 first increased and then decreased.These findings provide a theoretical foundation for further exploration of the physiological functions of CgTauTs and the molecular mechanisms underlying taurine accumulation in C. gigas.
  • Numerical investigation on the stability of OWC-perforated floating breakwater
    ZHENG Yanna , LI Jiafan, SHAO Xiran, MU Yingna, CHEN Changping, HAO Jian
    Journal of Dalian Fisheries University. 2024, 39(5): 856-863. https://doi.org/10.16535/j.cnki.dlhyxb.2024-016
    Abstract (276) PDF (44) HTML (0)   Knowledge map   Save
    To investigate the stability of an oscillating water column (OWC) perforated floating breakwater, the RAO (Response amplitude operator) for the motion of the floating breakwater was simulated using ANSYS AQWA software. The effects of several factors on breakwater stability were discussed including spacing between double-body structures of the breakwater, wave incident angle, width of OWC opening, draft depth, and length of anchor chain dragging. Results showed that the spacing change between double-body structures had little effect on mooring stability; an increase in wave incident angle led to reduce longitudinal sway motion amplitude and increase the stability; widening the OWC opening led to increased motion amplitude of the breakwater; increasing draft depth or reducing anchor chain length led to increase heave and roll direction motion amplitude while decreasing pitching direction motion and increasing natural period. The finding indicates that the mooring stability of OWC-perforated floating breakwater can be adjusted by changing the opening width, draft depth, length of anchor chain dragging, and wave direction in actual engineering.
  • Exploring low and Ultra-low temperature preservation techniques for sperm of Hemibarbus labeo in the Yalu River
    LI Jianqiao, LUO Xiaonian, DUAN Youjian, LI Jiao, KANG Junlong, MO Yanfang, DONG Ruixin, XI Wenqiu
    Journal of Dalian Fisheries University. 2025, 40(2): 204-215. https://doi.org/10.16535/j.cnki.dlhyxb.2024-223
    Abstract (275) PDF (120) HTML (0)   Knowledge map   Save
    To preserve the genetic resources of Hemibarbus labeo in Yalu River,ten mature parent fish (♀∶♂= 4∶6) were taken from Xingda Fish Farm in Liaoyang County in May 2024.The effects of various dilution solutions,dilution ratios,cryoprotectants,and freezing and thawing protocols on sperm were assessed.Results indicated that the dilution solution L-1 (0.8 g of NaCl,0.05 g of KCl,and 0.50 g of glucose dissolved in 100 mL of distilled water) had the superior preservation efficacy for H. labeo sperm.After 4 ℃ low temperature storage for 48 h,activation rates reached 71.667%±2.887%,motility times averaged (21.667±1.528) s,and lifespans were recorded at (41.000±1.000) s.The ideal dilution ratio was 1∶5,and post-storage recovery metrics showed activation rates of 76.667%±2.887%,motility times averaging (24.000±2.000)s,and lifespans of (39.333±1.528) s after low temperature storage for 48 h with a cryoprotectant (DMSO) concentration of 10%.The optimal freezing conditions for H. labeo sperm involved exposure to nitrogen vapor approximately 3 cm above the liquid nitrogen surface for 5 min prior to thawing.This treatment resulted in high vitality levels when 24-hour preserved sperm were thawed using a water bath set to 37 ℃ for 20 s and recovery metrics showed an activation rate of 80.000%±8.667%,average motility time of (37.000±1.733)s,and lifespan of (102.333±3.215)s.Following sperm recovery from the frozen state,artificial insemination trials yielded fertilization rates and hatching success rates of 70.667%±1.154% and 72.000%±4.583%,respectively.This study establishes a comprehensive system for low temperature and ultra-low temperature preservation techniques applicable to H. labeo sperm,thereby providing foundational data essential for both germplasm resources conservation and artificial reproduction of this species.
  • Analysis on karyotype of F1 hybrids of red seabream(Pagrus major)♂×black porgy (Acanthopagrus schlegelii)♀ and F1 self-fertilization of black porgy
    ZHOU Shimiao, XU Dafeng, DU Shuran, JIA Chaofeng, MENG Qian, ZHU Fei, CHEN Shuyin
    Journal of Dalian Fisheries University. 2025, 40(2): 216-222. https://doi.org/10.16535/j.cnki.dlhyxb.2024-212
    Abstract (272) PDF (102) HTML (0)   Knowledge map   Save
    To analyze the chromosome karyotype changes in F1 self-fertilization of Acanthopagrus schlegelii (AS) and F1 hybrids of AS ♀× Pagrus major (PA) and to provide a basis for black porgy breeding,metaphase chromosome morphology of two sub-populations were prepared respectively by injecting phytohemagglutinin(PHA) and colchicine.The results showed that the chromosome number of both AS and PA is 2n = 48.The karyotype of AS is 2n = 4m + 4sm + 2st + 38t (NF = 56),while the karyotype of PA is 2n = 8sm + 2st + 38t (NF = 56).They have some different karyotypes and st chromosomes with distinct satellite structures.The karyotype of AS is completely consistent with that of the black porgy female parent,while the karyotype of PA is inconsistent with that of the parent species.The number of st and t type chromosomes in the karyotype of PA is the same as that of the female parent,and total number of m+sm type chromosomes is also same.This might suggest gynogenesis for PA.The study provides reference information for the genetics and breeding of F1 hybrids and F1 self-fertilized black porgy.
  • Prediction method of pH value in aquaculture water quality based on the integration of LSTM and XGBoost
    GUO Fangyi, LIU Mingjian, WANG Gang, ZHANG Sijia, SHAN Bolin, LIU Tong
    Journal of Dalian Fisheries University. 2024, 39(6): 1021-1031. https://doi.org/10.16535/j.cnki.dlhyxb.2024-093
    Abstract (271) PDF (272) HTML (0)   Knowledge map   Save
    To ensure equilibrium of aquaculture ecosystem and health of aquatic animals, a pH prediction method for aquaculture water quality, designated as PCA-ES-LSTM-BSO-XGBoost (PELBX), was established. In the PELBX, principal component analysis (PCA) was firstly applied to reduce the dimensionality of water quality data, simplifying parameter complexity and enhancing the efficiency and accuracy of model training. Subsequently, the Long Short-Term Memory (LSTM) network was utilized to capture the dynamic changes in water quality parameters over time, employing early stopping to prevent overfitting and to ensure high prediction accuracy for unseen data. Moreover, the parameters of the XGBoost model in parallel were optimized by the BSO algorithm to improve the precision of pH predictions. Finally, the predictions from the LSTM and XGBoost models were weighted and combined, effectively integrating the advantages of time series analysis and nonlinear learning, significantly enhancing prediction accuracy. Experimental results showed that the PELBX model outperformed in pH prediction with a root mean square error of 0.115, mean absolute error of 0.088, mean absolute percentage error of 1.066%, and a coefficient of determination of 0.747. Compared to the best-performing PCA-LSTM-BSO-XGBoost model in ablation studies, the performance parameters above were improved by 8.73%, 8.33%, 8.26%, and 7.64% respectively; and relative to the best model in the field, BiLSTM-GRU, performances were improved by 10.16%, 1.12%, 0.56%, and 8.73% respectively. The finding demonstrates that the PELBX model significantly enhances the accuracy and stability of water pH value prediction, validating the effectiveness and feasibility of the proposed method.
  • Effects of seawater acidification on growth, and respiratory metabolism of mantis shrimp(Oratosquilla oratoria)
    SUN Peiqi, YANG Jisong, QIN Yuxue, XUE Jiaxin, CHANG Yifan, YU Xiaoming, CHEN Lei
    Journal of Dalian Fisheries University. 2024, 39(4): 589-596. https://doi.org/10.16535/j.cnki.dlhyxb.2023-287
    Abstract (266) PDF (301) HTML (0)   Knowledge map   Save
    To probe effects of seawater acidification on growth, feeding, and respiratory metabolism of mantis shrimp Oratosquilla oratoria, the mantis shrimp weighing (10.90±0.33) g was reared in 3 PVC cylindrical tanks of each 1.0 m height, and diameter 1.0 m, and exposed to pH 8.10 (control group) pH 7.90, pH 7.70,and pH 7.50 prepared by pure CO2 aeration for 49 days. During the experiment, the weight gain rate, specific growth rate, dressing rate, daily food intake, survival rate, as well as oxygen consumption rate and ammonia excretion rate of the mantis shrimp were determined. The results showed that there was significant impact of pH value of seawater on the weight gain rate, specific growth rate, dressing rate, daily food intake, survival rate, oxygen consumption rate, and ammonia excretion rate of the mantis shrimp (P<0.05), with decrease in weight gain rate, specific growth rate, and dressing rate of the mantis shrimp and increase in the daily food intake with the decrease in pH value. The oxygen consumption rate and ammonia excretion rate of the mantis shrimp were shown to be increased with the decrease in pH value, with a trend of first decreasing and then increasing in O∶N value. At the end of the experiment, there was survival rate of 98.41±0.72%, 98.10±0.68%, 97.74±0.80% and 95.31±1.25% of the mantis shrimp in each group, respectively. The significantly lower survival rate was found in pH 7.50 group than that in the control group (P<0.05). The findings indicated that acidification led to adverse effects on the feeding, growth and the respiratory metabolism of the mantis shrimp, with increase in the oxygen consumption rate and ammonia excretion rate of the mantis shrimp with the decrease in pH value.
  • Advancement in research and application of age determination in shellfish: a review
    GAO Jin, XIE Xi, BAI Yongan, PANG Jinchi, ZHAO Yanhong, WANG Qingzhi
    Journal of Dalian Fisheries University. 2024, 39(4): 708-718. https://doi.org/10.16535/j.cnki.dlhyxb.2023-269
    Abstract (265) PDF (251) HTML (0)   Knowledge map   Save
    Different methods of age identification for different shellfish species result in low efficiency, large errors, and low repeatability in specific research and practical processes due to the lack of unified standards and significant differences in morphological structures and lifestyles in shellfish species. In this article, the key development history of shellfish age identification technology, principles, characteristics, and application effects of different age identification techniques for shellfish are reviewed based on related literature on shellfish age identification at home and abroad. Suggestions are made regarding the verification of shellfish growth line formation cycles, optimal selection of suitable methods, and standardization process, aiming to provide references for research and innovative utilization of shellfish growth and age identification methods.
  • Comparative transcriptomics reveals key genes and underlying mechanisms of salinity-alkalinity tolerance in Gymnocypris przewalskii
    YAN Qingchun, LI Changzhong, WANG Linan, YANG Ying, LI Zhaonan, LIANG Tianxiu , JIN Wenjie, CHEN Yanxia
    Journal of Dalian Fisheries University. 2025, 40(2): 223-234. https://doi.org/10.16535/j.cnki.dlhyxb.2024-200
    Abstract (263) PDF (156) HTML (0)   Knowledge map   Save
    To further explore the regulatory mechanism of Gymnocypris przewalskii to adapt hypersaline and hyperalkaline water environment,we screened the genes that may play important regulatory roles in its adaptation to the hypersaline and hyperalkaline water environment of Qinghai Lake.We used transcriptomics technology to sequence the liver (GECL,GPRL),kidney (GECK,GPRK),and gill (GECG,GPRG) tissues of G. przewalskii and G. eckloni using high throughput sequencing.The different tissues of the two species were screened for differentially expressed genes (DEGs),which then were functionally annotated.The results showed that a total of 149.57 Gb of clean data were obtained from 18 samples,that the clean data from each sample reached >6.50 Gb,that the percentage of Q30 bases was >83.89%,and 224 010 Unigenes were totally obtained after de novo assembly.The GECG vs GPRG,GECK vs GPRK,and GECL vs GPRL comparisons yielded 9 807,6 550,and 5 651 DEGs respectively.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that DEGs co-expressed in gill,kidney,and liver tissues of G. przewalskii and G. eckloni were significantly enriched in the signaling pathways of “ferroptosis”,“apoptosis”,and “glycolysis/gluconeogenesis” (P< 0.05).The DEGs annotated to these pathways included genes such as tfa,tfr1a,atf4b,and hk2,which may play important regulatory roles in the adaptation of G. przewalskii to the hypersaline and hyperalkaline water environment of Qinghai Lake.
  • Effects of ocean warming on bacterial biofilm formation and biofilm-induced larval metamorphosis of the mussel Mytilus coruscus
    MA Fan, TAO Yu, YANG Jinlong, LIANG Xiao
    Journal of Dalian Fisheries University. 2025, 40(2): 241-249. https://doi.org/10.16535/j.cnki.dlhyxb.2024-205
    Abstract (259) PDF (87) HTML (0)   Knowledge map   Save
    The goal of this study was to assess how ocean warming regulates biofilm formation and larval metamorphosis of the mussel Mytilus coruscus. Pseudoalteromonas marina,a typical marine bacterium known for inducing larval metamorphosis,was selected as the test organism.The temperature gradient of 14 ℃,18 ℃,22 ℃,27 ℃,and 31 ℃ was set based on the sea surface temperature of the ocean combined with ocean warming.The characteristics of biofilms formed at different temperatures and their induction of larval metamorphosis were observed.The results showed that bacterial density,extracellular a-polysaccharide and cyclic dimeric guanosine monophosphate contents (c-di-GMP),and induction activity increased with increasing seawater temperature to a peak at 22 ℃,and then they decreased as temperature increased.The induction activity of biofilms was not correlated with β-polysaccharide content but was correlated with c-di-GMP content.Furthermore,the variation trends of lipopolysaccharide and colanic acid contents were consistent with the changes in biofilm induction capacity,whereas cellulose content showed an increasing trend with rising temperature.As c-di-GMP content regulates secretion of extracellular polysaccharides,temperature induced changes in its contents ultimately affect the capacity of the biofilm to induce larval metamorphosis.These results can be applied to improving the larval settlement efficiency of mollusks under ocean warming and to the sustainable development of the marine mollusk aquaculture industry.
  • Glucose metabolism and relevant response of Gymnocypris przewalskii exposed to chronic alkalinity stress
    GAO Zihan, WEI Wei, YUE Miao, LI Yixin, CUI Yanrong, XU Baoke, LI jin, WEI Fulei, LIANG Jian
    Journal of Dalian Fisheries University. 2025, 40(1): 65-75. https://doi.org/10.16535/j.cnki.dlhyxb.2024-042
    Abstract (259) PDF (93) HTML (0)   Knowledge map   Save
    To inquire mechanism of Gymnocypris przewalskii adaptation to alkalinity changes through glucose metabolism,G. przewalskii with body weights of 126.67 g±8.88 g were reared in an 85 cm×56 cm×62 cm tank containing either freshwater (control group) or four experimental alkalinities of 25%,50%,75% and 100%,prepared using NaHCO3 and Na2CO3 dissolved in tap water (NaHCO3∶Na2CO3 = 9∶1).The important indicators of G. przewalskii glucose metabolism were determined,including glycogen content,glucose (GLU) content,and the activities of pyruvate (PA),hexose kinase (HK),pyruvate kinase (PK),phosphoenolpyruvate (PK),phosphoenolpyruvate carboxylase (PEPCK),glucose 6 phosphate dehydrogenase (G6PD),and fructose 1,6 bisphosphate aldolase (FBA).The results showed that,with increasing alkalinity,the hepatic glycogen,muscle glycogen,and GLU content in the gills and kidneys increased and then decreased,and the GLU in the muscle gradually decreased.As the alkalinity increased,PA in the gills increased and then decreased,and PA in the kidneys,liver,and muscle decreased and then increased.The activities of HK,PK,PEPCK and G6PD in the gills showed an increasing trend with increasing alkalinity,and the activities of FBA in the kidneys and liver gradually rose.A heatmap showed that glucose metabolism processes in the gills,kidneys,and liver were promoted by increasing alkalinity to some extent,while glucose metabolism in the muscle was inhibited as alkalinity increased.As alkalinity increased,fba-c was commonly expressed in the gills,kidneys,intestine,and liver,and the expression level of fba-c in the gills and kidneys increased and then decreased.The maximum expression levels were observed in the 75% alkalinity group.In conclusion,the gills,kidneys,and liver responded to alkalinity changes by increasing glucose metabolism,while muscle responded to alkalinity changes by inhibiting glucose metabolism,leading to rapid adaptation to different alkalinity levels in G. przewalskii.
  • Research progress on typical red tide organism Noctiluca scintillans:a review
    CHEN Yifan, WANG Junyue, WANG Yinghao, LI Qiuping, HE Senhao, SHEN Xin, JI Nanjing
    Journal of Dalian Fisheries University. 2024, 39(6): 1075. https://doi.org/10.16535/j.cnki.dlhyxb.2024-045
    Abstract (259) PDF (803) HTML (0)   Knowledge map   Save
    In this review, the research progress on Noctiluca scintillans was summarized, including basic biology, ecology, and red tide governance. Furthermore, future researches were proposed focusing on feeding, reproduction, physiology, biochemistry, and early warning and control of N. scintillans considering the challenges present in the current research on this species, aiming to offer theoretical perspectives on the mechanisms involved in bloom formation and prevention of red tides caused by N.scintillans.
  • Transcriptomic response of the Pacific abalone(Haliotis discus hannai) to different algae diets and preliminary functional analysis of related sulfatase gene ids-1
    HU Taoze, XIE Yusu, LI Chao, YAO Yibing, LIU Xiao, XU Fei
    Journal of Dalian Fisheries University. 2024, 39(6): 915-925. https://doi.org/10.16535/j.cnki.dlhyxb.2024-063
    Abstract (258) PDF (306) HTML (0)   Knowledge map   Save
    In order to analyze the decomposition and adaptation mechanism of Pacific abalone (Haliotis discus hannai) to its ingested algae,the differentially expressed genes (DEGs) in the viscera mass of the Pacific abalone fed three types of algae (Laminaria japonica, Gracilaria lemaneiformis and Ulva pertusa) as well as the selected sulfatase gene ids-1, were investigated by transcriptome sequencing, bioinformatics analysis, gene cloning, quantitative real-time PCR, prokaryotic expression, protein purification, and enzymatic activity analysis. The results showed that a total of 104 up-regulated and 118 down-regulated DEGs were identified when comparing the group LXC to HD. A total of 80 up-regulated genes and 93 down-regulated genes were identified in the group SC to HD, while the number of up-regulated and down-regulated genes in the group SC to LXC were 77 and 72. The results of Gene Ontology (GO) showed that the DEGs were enriched to entries mostly related to substance metabolism, especially the synthesis, catabolism, and metabolism of sulfated substances, with several sulfatase genes that showed DEGs identified at GO:0006027 (glycosaminoglycan catabolic process). Further, the sulfatase ids-1 gene was cloned and its molecular characteristics were analyzed. The open reading frame of this gene was determined to be 1 767 bp in length, encoding 588 amino acids. The deduced protein contains an N-terminal signal peptide (1-23 aa), a conserved domain of sulfatase (28-376 aa), and 9 glycosylation sites. Phylogenetic analysis showed that IDS-1 from pacific abalone shared the highest homology with IDS from Haliotis refescens and Haliotis rubra. Multiple amino acid sequence alignment demonstrated that IDS-1 carries the typical core motif “CXPXR” of the sulfatase family as well as the highly conserved active sites of the IDS subfamily. However, the recombinant IDS-1 protein produced from Escherichia coli did not exhibit sulfatase activity. Furthermore, qPCR analysis showed the highest expression of ids-1 in the visceral mass of pacific abalone, followed by the gill and mantle, with comparatively lower expression in upper foot tentacles. Our study also demonstrated a developmental stage-specific expression pattern of ids-1. The expression level is low from fertilized eggs to planktonic larvae, and increases in plantigrade larvae, with the highest level in adults. Additionally, we found that the expression of ids-1 varied significantly under different algae feeding conditions, with the brown alga-fed group exhibiting notably higher expression compared to the red and green alga-fed groups, while the green alga group displayed the lowest expression level. We speculate that ids-1 may be closely related to the nutritional patterns and dietary transitions during the development of abalone, and its expression level is potentially directly affected by differences in feeding preferences and food intake. This findings provides reference for in-depth analysis of the diet adaptation regulation mechanism in Pacific abalone.
  • Screening and identification of a highly efficient denitrifying functional bacterium XYB4 and its effects on the growth and intestinal flora of Litopenaeus vannamei and water quality
    WU Jiaqi, ZHOU Jianing, XUE Yuan, WANG Furui, ZHU Miao, SUN Chengbo
    Journal of Dalian Fisheries University. 2025, 40(1): 86-94. https://doi.org/10.16535/j.cnki.dlhyxb.2024-126
    Abstract (253) PDF (115) HTML (0)   Knowledge map   Save
    To develop highly efficient and economic denitrifying technology for an aquaculutre system,the experiment isolated a highly efficient denitrifying bacterium from a biofloc in shrimp culture pond ( named as XYB4) by morphological screening,physiological and biochemical identification,molecular biological assay,safety evaluation test,and denitrifying effect under the influence of different physical and chemical factors.The result shows that the XYB4 strain is a gram-positive bacillus.16S rDNA sequence result shows that it is Bacillus subtilis.The safety evaluation test shows that XYB4 is not lethal to Litopenaeus vannamei,without hemolysis,and is sensitive to 14 types of antibiotics.The best conditions for denitrification by the XYB4 strain are as follows:salinity 30,temperature 30 ℃,pH 7.5,and C/N 10-15.A mixture of XYB4 with feed (B7),splashed into an aquaculture pond (P7),significantly reduced the concentrations of  and  in the cultured water (P<0.05),and significantly increased the growth rate and specific growth rate of L. vannamei by 16.32%,17.52%,and 40.18%.and 40.06%,respectively.There was no significant difference in survival rate among the three groups (P>0.05).The richness of the L. vannamei intestinal flora and the water flora is significantly increased in the experimental group (P<0.05).The addition of the XYB4 strain inhibited the growth of harmful bacteria,such as Photobacterium and Tenacibaculum,and promoted the growth of beneficial nitrifying bacteria,such as Nitrospirae.In conclusion,the application of XYB4 can effectively reduce the concentrations of  and  in aquaculture water,promote the growth of L. vannamei,improve the richness of the microflora communities in the water and L. vannamei intestines,and inhibit the growth of some harmful microbial populations.The XYB4 has a high application value in aquaculture production.
  • Research progress and prospect of solid waste transport performance in recirculating aquaculture system:a review
    ZHAO Chenxu, WU Gang, SUN Wei, HU Wei, REN Xiaozhong, ZHANG Jun, WANG Xian
    Journal of Dalian Fisheries University. 2025, 40(1): 174. https://doi.org/10.16535/j.cnki.dlhyxb.2024-161
    Abstract (252) PDF (63) HTML (0)   Knowledge map   Save
    Recirculating aquaculture systems (RAS) are widely acknowledged as pivotal in developing future improved aquaculture practices because of their ability to rear a large number of aquatic organisms in a small space,conserve resources,and maintain environmental sustainability.Nevertheless,the buildup of solid waste in RAS presents a danger to the health of aquaculture species and has detrimental impacts on the functioning and productivity of a system.Efficient management and disposal of solid waste in RAS is crucial for maintaining optimum system operation.This study examines the categorization,origins,and potential consequences of solid waste in the RAS,and also explores methods to enhance waste transportation efficiency.Significant improvements in solid waste removal efficiency can be achieved by optimizing the design of aquaculture tanks,upgrading intake and drainage structures,modifying tank bottom gradients,utilizing diversion devices,and enhancing feed quality.These improvements will guarantee the consistent and effective functioning of a RAS.
  • Study on the response of polian vesicle to Vibrio splendidus invasion in Apostichopus japonicus
    JIA Ziqiang, LI Qiang, REN Yuan, WU Jiong, WANG Yinan
    Journal of Dalian Fisheries University. 2025, 40(2): 235-240. https://doi.org/10.16535/j.cnki.dlhyxb.2024-198
    Abstract (249) PDF (88) HTML (0)   Knowledge map   Save
    To investigate the response of polian vesicles to pathogenic bacteria invasion in the sea cucumber Apostichopus japonicus,we injected Vibrio splendidus,the pathogen responsible for “skin ulcer syndrome”,into the coelom of A. japonicus via the body wall,and studied the morphological and tissue structural changes of polian vesicles,and proliferation rate of cells in polian vesicles’ wall and cavity.The results showed that the morphology and tissue structure of polian vesicles changed significantly after invaded by V. splendidus.During the 2 h to 12 h after injection of Vibrio splendidus,polian vesicles expanded and elongated gradually,the number of dark-stained cells in the polian vesicles’ wall first decreased,then increased,but decreased,then increased again,and their proliferation was most active within 6 h to 12 h after injection.The 5-ethynyl-2′-deoxyuridine (EdU) positive rate of cells in the cavity of polian vesicles was 10.39%,11.20% and 11.61%,respectively,at 4 h,6 h,and 12 h after injection,whereas it was 7.22%,6.24%,and 6.61%,respectively,in the control group.These results indicated that polian vesicles responded to V. splendidus invasion by accelerating cell proliferation in the cyst wall and cavity,even if the bacteria could not enter the vesicles.
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Editor-in-Chief: SONG Linsheng
Deputy Editor-in-Chief:
ZHANG Guochen
LI Zhijun
LIU Ying
ZHANG Dongdong (executive)
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Supervisor: Education Department of Liaoning Province
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