The grow th and petroleum degradation characteristics of bacteria in coastal beach
WANG Bin1,2,ZHOU Ya-fei1,ZHANG Xing1,ZHENG Feng-wei1,GAO Yu-ting1,HE Jie1,ZHOU Yi-bing1,2
1.Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023, China;2.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province,Dalain Ocean University,Dalian 116023,China
Abstract: The bacteria that have capability to degrade crude oil were isolated from the coastal beach polluted by crude oilwith the basalmedium crude oil as only carbon source.The original degradation rate of diesel oil by the isolated strains was determined.In addition,the degradation rate of diesel oil by the isolated strain was determined with the basalmedium diesel oil including different concentration of glucose(0,1,2,4,8,16 g/L),the different concentrations of yeast extract,peptone,carbamide,(NH4)2SO4(according to the content of nitrogen:0.5, 1,2 g/L)and NaH2PO4(0,4,8,16 g/L).The isolated strain was identified and growth characteristicswere studied.The results showed:5 strains bacteria grew on the basalmedium crude oil as only carbon source.One of these stains had amaximum original degradation rate(19.0%)and was numbered as Y-3.The degradation rate of Y-3 increased and reached to 79.9%in the diesel oilmedium supplemented with glucose(4 g/L).The degradation rate was increased in Y-3 in addition of yeastextractand peptone also,butnotnotable in added urea,(NH4)2SO4and NaH2PO4.The Y-3 was identified asPseudomonasputidaaccording to the resultof themorphology,physiological characteristics and 16S rDNA sequence analysis.The Y-3 had fitting growth at temperature of 30℃,pH 8, and NaCl concentration of 0-30 g/L.
2012, Vol. 27 
