Adaptative expression of four transporter-related genes in sea cucumber Apostichopus japonicus exposed to salinity stress
JIANG Ya-nan, TIAN Yi, LI Xiao-yu, SHANG Yan-peng, CHANG Ya-qing
Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
Abstract: The expression profiles of four genes related-salinity regulation were detected in intestine, coelomic fluid, and respiratory tree of sea cucumberApostichopusjaponicas with body weight of (16.93±3.08)g reared at a salinity of 30 (as control group) which were abruptly exposed to low salinity of 18 for 1.3, 3,6, 12, 24, 48 and 72 h by RT-PCR, including single carboxylic acid transporter family 16a13 (Monocarboxylate Transporters 13, SLC16a13) gene, single carboxylic acid transporter family 6a8 (Solute Carrier Family 6 member 8, SLC6a8) gene, Chitin receptor protein (Fibrinogen C Domain-Containing Protein 1, FIBCD1) gene, and AMPA type glutamate receptor 1 (Glutamate Ionotropic Receptor AMPA Type Subunit 1, Gria1) gene. The results showed that there was expression of the 4 genes in coelomic fluid, intestine and respiratory tree significantly tree higherSLC16a13 expression level than that in the control group at 6 h, 12 h, and 48 h(P<0.05), except in the intestine at 1.5 h and 3 h, with the maximum at 72 h. In the respiratory tree, the significantly up-regulatedSLC16a13 expression was shown, the maximal expression at 72. During the experiment,SLC16a8 gene was shown to be significantly higher in coelomic fluid that in the other tissues(P<0.05). The maximalFIBCD1 expression level was observed in coelomic fluid, followed by the respiratory tree, and the minimum in the intestine. There was the maximalGria1 expression in the intestine, followed by coelomic fluid and the minimal in the respiratory tree. The findings appear to be the foundation for research of adaptation mechanism of sea cucumber.
2018, Vol. 33 
