WEI Ping-ping1,2, LI Xin1,2, FEI Fan1,2, MA He1,2, GAO Dong-kui1,2, SONG Chang-bin3, CHEN Tao4, LIU Ying1,2,5*
1.College of Marine Science and Environment， DaLian Ocean University, Dalian 116023, China； 2.Key Laboratory of Environment Controlled Aquaculture Ministry of Education, Dalian 116023, China; 3.Institute of Semiconductors China Academy of Sciences, Beijing 100083,China； 4.Led Cooler Technology Company Limited, Shenzhen 518000, China; 5.Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China
Abstract: Postlarval tiger puffer Takifugurubripes with body length of (4.09±0.39)mm were reared in a round barrel with 80 cm in diameter and 50 cm in height under various light spectra from LED emission including white(λ400-780 nm), blue(λ450-455 nm), green(λ525-530 nm), yellow(λ590-595 nm), and red (λ625-630 nm)to explore the effects of light spectrum on growth and related gene expression of tiger puffer larvae. The changes in relative expression levels of the related genes including growth hormone gene, growth hormone receptor 1 gene, insulin-like growth factor 1 gene, growth hormone-relasing hormone 1 gene and somatostatin 1 gene were detected by real-time fluorescence quantitative PCR. The results showed that there were higher body length and body weight in the larval tiger puffer exposed to yellow light spectrum than those in other light colors, followed by the blue, white and green light exposure fishes, with significant differences among different light colors(P<0.05). The expression level of GH gene was found to be significantly higher in red light group than that in blue, yellow and white groups at 12 d (P<0.05), without significant difference between the two groups (P>0.05). The expression level of GHR1 gene was significantly higher in green light group than that in other groups at 12 d (P<0.05). The expression levels of IGF1 gene were shown to be significantly higher under green light, yellow light and red light than those under green light and white light (P<0.05), and the expression levels of GHRH1 gene were significantly higher in green light and red light groups than those in white and yellow light groups at 12 d (P<0.05). The expression level of SS1 gene was significantly higher in red light group than that in other treatment groups (P<0.05), with the descending order as red>blue>green>white>yellow. At 22 d, however, almost all of the larvae were found to be died in the red light group, while the expression level of GH gene was higher in the green light group than that in the blue and yellow light groups (P<0.05), without significantly different from the white light group (P>0.05). No significant difference in the expression levels of GHR1 and GHRH1 genes was observed in different spectrum groups at 22 d (P>0.05). The expression level of IGF1 gene was significantly higher in green light group than that in other groups (P<0.05), with the maximal relative expression level of SS1 gene under yellow light group, significantly higher than those in other light color groups (P<0.05). The findings indicate that yellow light has promoting effect on growth and development of tiger puffer larvae, and that red and green light are not suitable for survival of the larvae.
2019, Vol. 34 
