Abstract: Fourteen morphological traits and correlation were comparatively measured and analyzed in 6-month-old tiger puffer Takifugurubripes in three families by cluster analysis, One-way ANOVA and correlation analysis in order to obtain excellent families and morphological traits of the tiger puffer and provide data for breeding. The results showed that there were higher body weight(BWH), body depth (BD), body width (BW) and caudal peduncle depth (CPD) in the full-sib family than those in the Japanese family and the half-sib family, significantly higher than those in Japanese family (P<0.05), with better growth than that in the Japanese family and the half-sib family. The cluster analysis showed that the Japanese family had significantly different morphology from the full-sib family and half-sib family, and phylogenetic analysis revealed that half-sib family and full-sib family were clustered into one branch, and then another converged Japanese family. The One-way ANOVA indicated that there were significant differences in postorbital head length (EH), head length (HL), BWH, HL + trunk length (TR) and occipital (IS) among the morphological traits of tiger puffer in three families (P<0.05). Correlation analysis showed that total body length(TL), body length (BL), BD, BW, HL+TR, caudal length (TA) and CPD were shown to be significantly correlated with BWH (P<0.05). The findings indicate that full-sib families can be considered as excellent families for genetic breeding of tiger puffer, and that the BWH, BW, BD, BL, HL and CPD are the main breeding traits, combined with the significant differences and the coefficient of variation in morphological traits related to BWH in the three families, which can provide reference for breeding of tiger puffer.