Abstract: The purpose of this experiment was to investigate the effect of GHRH(growth hormone-releasing hormone)gene on the growth traits of tiger puffer Takifugurubripes and to provide reference for molecular assisted breeding.The sequences of GHRH gene were analyzed and aligned in tiger puffer whose body mass,body length,body height and other 10 growth-related traits were measured at the age of 6 months and 12 months after breeding by designed four pairs of PCR primers to cover the whole sequence of GHRH gene to screen the single nucleotide polymorphisms (SNPs).Subsequently,association analysis was performed between the SNPs and growth traits as well as linkage disequilibrium and diplotype analysis.The results showed that a total of seven SNPs sites were screened out,including C81T,G315C,C1083T,A1524G,G2107A,T2125C and G2256A,in which C81T,G315C and A1524G were significantly correlated with most growth traits.G2256A and T2125C,G207A and C083T were in linkage disequilibrium but not complete linkage.The diplotypes D4 and D5 had significantly higher mean body mass than the diplotype D3 did (P<0.05).The three SNPs of C81T,G315C and A1524G were shown to be dominant loci with a positive effect on the growth of tiger puffer,and the diplotypes D4 and D5 were the dominant genotypes in 6 month old population,while the diplotype D5 was the dominant genotype in 12 month old population,which was preferred in the subsequent breeding process of tiger puffer.
王梓祎, 苟盼盼, 杨金, 马明星, 杨诗晨, 李楠, 仇雪梅. 红鳍东方鲀生长激素释放激素(GHRH)基因多态性及其与生长性状的关联分析[J]. 大连海洋大学学报, 2022, 37(1): 34-41.
WANG Ziyi, GOU Panpan, YANG Jin, MA Mingxing, YANG Shichen, LI Nan, QIU Xuemei. Correlation analysis between single nucleotide polymorphisms (SNPs) of GHRH gene and growth traits of tiger puffer Takifugu rubripes. Journal of Dalian Ocean University, 2022, 37(1): 34-41.
2022, Vol. 37 
