Effects of photoperiod on expression level and daily expression pattern of GH and SS genes in brain of tiger puffer Takifugu rubripes
WEI Pingping 1,2, LI Xin 1,2, LIU Ying 1,2,3, LI Xiaohao 1,2, SONG Changbin 4, CHEN Tao 5, MA He 1,2* , TIAN Ye 1,2
1.College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China; 2.Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian 116023, China; 3.Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China; 4.Institute of Semiconductors, China Academy of Sciences, Beijing 100083, China; 5.Led Cooler Technology Company Limited, Shenzheng 518172, China
Abstract: Juvenile tiger puffer Takifugu rubripes with initial body weight of (7.89±0.42)g was reared in a barrel with a depth of 50 cm and a diameter of 80 cm at a rate of 60 fish per barrel and exposed to photoperiod of 8L∶16D, 12L∶12D, 16L∶8D, 20L∶4D, and 24L∶0D at water temperature of 19-23 ℃ for 30 d with triplication. The growth hormone (GH) and somatostatin (SS) genes were analyzed in brains of tiger puffer to investigate effects of photoperiod on expression level and daily expression pattern of GH and SS genes in brain of tiger puffer. The expression of GH gene was shown to be significantly higher in brains of tiger puffer in 8L∶16D group than that in other groups (P<0.05), significantly higher expression level of SS gene in 8L∶16D group than at in 24L∶0D group (P<0.05), without significant difference in the expression levels of SS genes in the other groups. The expression levels of GH and SS gene were low at light phase and high at dark phase under 12L∶12D and 8L∶16D, respectively. The findings indicate that long-term light (16-24 h/d) is not conducive to the maintenance of the physiological rhythm of juvenile tiger puffer and that the optimal duration of illumination iss 8-12 h/d in the experiment.
魏平平, 李鑫, 刘鹰, 李晓豪, 宋昌斌, 陈涛, 马贺, 田野. 光周期对红鳍东方鲀脑组织中GH和SS基因表达水平和昼夜表达模式的影响[J]. 大连海洋大学学报, 2020, 35(1): 108-113.
WEI Pingping , LI Xin , LIU Ying , LI Xiaohao , SONG Changbin , CHEN Tao , MA He , , TIAN Ye , . Effects of photoperiod on expression level and daily expression pattern of GH and SS genes in brain of tiger puffer Takifugu rubripes. Journal of Dalian Ocean University, 2020, 35(1): 108-113.
2020, Vol. 35 
