1.College of Fisheries and Life Science，Dalian Ocean University，Dalian 116023，China；2.Beijing Fisheries Research Institute，Beijing 100068，China；3.Beijing Key Laboratory of Fishery Biotechnology，Beijing 100068，China
Abstract: Thirteen microsatellite lociwere amplified from sterlet Acipenser ruthenus using published cross-species primers for polymorphism analysis.A proportion method and a filling method were comparatively used for reading data derived from microsatellite in order to find the suitablemethod.The results showed that there was no significant difference（P＞0.05）between the twomethods so that fillingmethod was chosen for the genetic diversity analysis in the following study.The observed number of alleles per locuswas ranged from 5 to 30，and the levels of observed and expected heterozygosities for polymorphic lociwere changed from 0.089 6 to 0.940 3 and from 0.085 2 to 0.912 6，respectively，with average of0.648 0 and 0.719 3.Therewas average Hardy-Weinberg departure value（d）of-0.098 0 and average polymorphism information content（PIC）of0.702 8，indicating that polymorphic lociwere all suitablemolecularmarkers for genetic analysis of sterlet population.The genetic diversity analysis revealed that the test farmed sterlet population had inbreeding to a certain extent.It is suggested that the genetic diversity of the population be improved by introduction of different broodstocks.
董颖, 杨瑞, 姜志强, 胡红霞. 微卫星标记在人工养殖小体鲟种群的数据分析方法比较和遗传多样性分析[J]. 大连海洋大学学报, 2016, 31(5): 516-532.
DONG Ying, YANG Rui, JIANG Zhi-qiang, HU Hong-xia, . Com parativemethods of reading data derived from genetic diversity analysis by m icrosatellite loci in farmed sterlet Acipenser ruthenus population. Journal of Dalian Ocean University, 2016, 31(5): 516-532.
2016, Vol. 31 
