QUE Yan-fu1， XU Xian2， XU Nian1， HUANG Jin3， LI Wei-tao1， XIONG Mei-hua1， LI Hong-tao2， ZHU Bin1*
1.Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem,Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China; 2.Wuhan Sinoeco Ecological Science & Technology Company Limited, Wuhan 430080, China; 3. Breeding and Releasing Station of Fish in Angu Hydropower Station, Shengda Hydropower Company Limited, China Hydropower Construction Group, Leshan 614013, China
Abstract: In the present study, six polymorphic microsatellite markers were first used in parentage analysis for the two families of Hemibarbuslabeo at Angu Proliferation Releasing Station. A total of 37 alleles were obtained, with the observed heterozygosis ranging from 0.361 to 0.952 (mean value 0.776), expected heterozygosis varying from 0.302 to 0.795 (mean value 0.589), and polymorphic information content(PIC) ranging from 0.262 to 0.767 (mean value 0.526). The Cervus analysis indicated that there was combined non-exclusion probability (CEP) of 99.09% if parents were both unknown. The parentage allocation rate was 95.83% (12 parents) in 72 offsprings of the two families, with above 95% of both CEP and parentage allocation rate, which is a basic requirement value for parentage analysis. The finding shows that these six microsatellite markers are useful for parentage identification, and provides technical support for evaluating stock enhancement in Hemibarbuslabeo in the future.
2019, Vol. 34 
