Genetic diversity and genetic differentiation in broodstock, released populations and natural fishing population of black bream Megalobrama terminalis in the Qiantang River based on COⅠ gene
1.Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 2.College of Fisheries, Nanjing Agricultural University, Wuxi 214081, China;3.Hangzhou Supervision and Management Chief Station of Fishery Administration, Fishing Port and Fishing Boat, Hangzhou 310008, China
Abstract: In order to probe into the status of genetic diversity and genetic differentiation of broodstock, released populations and natural fishing population of black bream Megalobramaterminalis in the Qiantang River, mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ)gene was sequenced in 224 individuals from 6 populations including 2 broodstock populations collected from Jingshan(JS)and Bai Song(BS), 3 released populations Yuhang(YH), Tonglu(TL)and Jiande(JD)and 1 natural fishing population from capture.The results showed that 192 variable sites accounting for 29.05% of total nucleotide were found in 661 bp sequences，with average content of 26.5% in A，28.0% in T，29.0% in C and 16.5% in G.There was higher content of(A+T)(54.5%)than that of(G+C)(45.5%), and 31 haplotypes were detected in total, in which 6 haplotypes were shared by at least two populations, with the first hap9 dominant haplotypes, accounting for 48.66% and the second hap2 dominant haplotypes, accounting for 22.32% of the total samples.Twenty-five specific haplotypes(only accounting for 13.4% of the total number of samples)were found in only one population, which was consistent with the result that hap9 was the original haplotype based on the haplotype network structure diagram.The six populations had haplotype diversity(Hd)range of 0.710-0.848(average 0.705), and the nucleotide diversity(Pi)range of 0.003 07-0.008 40(average 0.005 78), with the maximal genetic diversity in Jingshan broodstock population, and the minimal genetic diversity in Yuhang released population.The genetic differentiation coefficient(Fst)was shown to vary from-0.009 34 to 0.193 55(average 0.099 09)among the 6 populations, with the maximal values of gene flow(Nm)of over 1.AMOVA analysis showed that there was 90.09% of genetic variation within the populations, and 9.91% genetic variation among populations.There was Nei’s genetic distance of 0.003-0.009 among the 6 populations, with higher genetic diversity in black bream in the Qiantang River, indicating that there was moderate genetic differentiation among some populations, and more gene exchange among populations, without significant genetic differentiation between natural fishing population and farmed population.
王邢艳, 徐东坡, 张婉平, 童奇烈, 方弟安, 周彦锋, 张敏莹. 基于COⅠ基因研究钱塘江三角鲂亲本、放流和自然捕捞群体的遗传多样性与遗传分化[J]. 大连海洋大学学报, 2021, 36(4): 603-611.
WANG Xingyan, XU Dongpo, ZHANG Wanping, TONG Qilie, FANG Di’an, ZHOU Yanfeng, ZHANG Minying. Genetic diversity and genetic differentiation in broodstock, released populations and natural fishing population of black bream Megalobrama terminalis in the Qiantang River based on COⅠ gene. Journal of Dalian Ocean University, 2021, 36(4): 603-611.
2021, Vol. 36 
