cDNAs cloning and expression of egg zona pellucida ZP3s and ZPAX genes in various tissues of sterlet Acipenser ruthenus
DONG Tian， ZHU Hua， WANG Wei， DONG Ying， TIAN Zhao-hui， HU Hong-xia
Beijing Key Laboratory of Fishery Biotechnology, Beijing Fisheries Research Institute and National Engineering Research Centre for Freshwater Fisheries, Beijing 100068, China
Abstract: The partial-length cDNA sequences of?ArZP3s(ArZP3-1,?ArZP3-4 and?ArZP3-5) and?ArZPAX?genes are cloned in sterlet?Acipenser?ruthenus?by PCR to identify the gonadal development mechanism of zona pellucida gene family in sterlet. It was found that?ArZP3-1 was 1029 base pairs (bp), predicting proteins of 342 amino acid residues;?ArZP3-4 1191 bp in length, predicting proteins of 397 amino acid residues;?ArZP3-5 717 bp in length, predicting proteins of 238 amino acid residues; and?ArZPAX?733 bp in length, predicting proteins of 243 amino acid residues. Sequence analysis revealed that these genes were all comprised of a conserved ZP domain. Phylogenetic tree indicated that three?ArZP3s of sterlet showed a closer genetic relationship with higher fishes, and?ArZPAX?was clustered with its counterparts in other vertebrates. Semiquantitative RT-PCR demonstrated that the?ArZP3-1 was expressed in ovary, testis, liver, brain, kidney, heart, white muscle and gill,?ArZP3-4 only in ovary and testis, and?ArZPAX?was detected in ovary, testis, liver, intestine, kidney, spleen, heart, brain and gill, with the maximal expression level in the gonads and differentially sexual expressed.
东天, 朱华, 王巍, 董颖, 田照辉, 胡红霞. 小体鲟卵透明带家族ZP3亚型和ZPAX基因cDNAs克隆及其组织表达分析[J]. 大连海洋大学学报, 2018, 33(4): 444-454.
DONG Tian, ZHU Hua, WANG Wei, DONG Ying, TIAN Zhao-hui, HU Hong-xia. cDNAs cloning and expression of egg zona pellucida ZP3s and ZPAX genes in various tissues of sterlet Acipenser ruthenus. Journal of Dalian Ocean University, 2018, 33(4): 444-454.
2018, Vol. 33 
