Functional divergence of different ep300/crebbp gene family members in rainbow trout Oncorhynchus mykiss
1.College of Animal Science,Shanxi Agricultural University,Taigu 030801,China;2.Agriculture and Rural Affairs Bureau of Lüliang City,Lüliang 033099,China
1.College of Animal Science,Shanxi Agricultural University,Taigu 030801,China;2.Agriculture and Rural Affairs Bureau of Lüliang City,Lüliang 033099,China
Abstract: To probe into copy number variation,functional divergence and correlation with hypoxia response of ep300/crebbp gene family in rainbow trout Oncorhynchusmykiss,TBLASTN and BLASTP search and reconstruction of phylogenetic tree were firstly conducted,based on which the members and their orthologs in zebrafish Daniorerio were determined.Tissue expression profiles and co-expressed genes of these copies were analyzed based on original transcriptome sequencing data,and GO enrichment analyses of these co-expressed genes were also conducted.The degree of variation among proteins encoded by these copies was compared by CDD search,multiple sequence alignment and three-dimensional structural modeling.The results showed that there were eight ep300/crebbp members in rainbow trout (ep300aa-ep300bb,crebbpaa-crebbpbb),which were all formed by whole genome duplication events and could be divided into four ohnolog pairs corresponding to the four members in D.rerio.There were significant differences in tissue expression profiles,and primary and tertiary structures of encoded proteins between different ohnolog pairs,much smaller differences between two copies within an ohnolog pair.These eight members had different numbers of unique co-expressed genes,and the number of GO terms enriched by these gene collections related to binding,modification,stress/stimulus-response and hypoxia tolerance were also varied greatly.ep300ba and ep300bb belong to an ohnolog pair,and yet their encoded proteins were varied in 35 positions in conserved domains,with the far greater number than other ohnolog pairs.Furthermore,only co-expressed genes of ep300bb enriched GO terms related to stress/stimulus-response and hypoxia tolerance.The findings suggest that ep300bb can serve as an important candidate gene for genetic improvement of rainbow trout due to its great evolution rate and stronger correlation with hypoxia tolerance than other ep300/crebbp members.
王宪宗, 刘青, 李澍. 虹鳟ep300/crebbp基因家族不同拷贝的功能分化[J]. 大连海洋大学学报, 2022, 37(3): 376-385.
WANG Xianzong, LIU Qing, LI Shu. Functional divergence of different ep300/crebbp gene family members in rainbow trout Oncorhynchus mykiss. Journal of Dalian Ocean University, 2022, 37(3): 376-385.
2022, Vol. 37 
