Effects of florfenicol on relative expression of ATP-binding cassette transporter gene(ABCG)in different tissues of sw imm ing crab Portunus trituberculatus
1.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;2.Yellow Sea Fisheries Research Institute,Chinese Acad-emy of Fishery Sciences,Qingdao 266071,China
Abstract: ATP-binding cassette transporter genewas cloned in swimming crab Portunus trituberculatus by a RACE method and the gene expression of ABCG was in hepatopancreas,gill and muscle of swimming crab with intramus-cular injection of flofenicol ata dose of20,40 and 80mg/kg to evaluate effects of florfenicol on the gene expression of ABCG in different tissues of swimming crab by fluorescent quantitative PCR.Results showed the full-length of ABCG cDNA of swimming crab(ptABCG)was shown to be 2473 bp encoding a polypeptide of 578 amino acids. Bioinformatics analysis indicated that ptABCG,without signal peptide,sharedmany similaritieswith the ABC trans-membrane transporter,including two conserved regions:a highly conserved nucleotide binding domains(NDB) and a transmembrane domain(TMD).Homology and phylogenetic analysis revealed that the amino acid sequence of ptABCG shared a high similarity with Chinese shrimp Fenneropenaeus chinensis and Pacific white leg shrimp Litopenaeus vannamei.The gene expression of ptABCG was up-regulated in hepatopancreas,gill and muscle after intramuscular injection of different doses of florfenicol,showing time-dose effect.It is speculated that the ptABCG protein may involve in themetabolism of florfenicol in swimming crab.
2017, Vol. 32 
