Vitellogenin（Vg）gene in mantis shrim p Oratosquilla oratoria
LIU Hai-ying1，2，ZHANG Na1，2，YAN Hong-wei3，LIU Qi1，2，XING Kun1，2，CHEN Lei1，2，SUIYou-zhen4，LIN Yuan5，LICheng-jiu5，GUO Liang-yong5
1.Center for Marine Ranching Engineering Science Research of Liaoning Province，Dalian Ocean University，Dalian 116023，China；2.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province，Dalian Ocean University，Dalian 116023，China；3.College of Fisheries and Life Science，Dalian Ocean University，Dalian 116023，China；4.InstituteofOcean Fisheries，Zhoushan 316021，China；5.Fisheries Seedlings Authority of Liaoning Province，Dalian 116015，China
Abstract: In the present study，the full length cDNA of vitellogenin（Vg）gene was cloned in mantis shrimp Oratosquilla oratoria using rapid amplification of cDNA ends（RACE）（GenBank accession No.KR422400），and expression levels of Vg genemRNA in various tissues，especially in ovary within differentovarian development stages，were investigated by qPCR.The results showed that the Vg gene was 7727 bp long，with 36 bp of 5′untranslated region（UTR），170 bp of 3′UTR and 7521 bp of the coding region encoding a size of 2506 amino acids predicted protein.The predicted protein contained a signal peptide（20 amino acids long），three N-glycosylation site，a consensus cleavage site RSKR and a conserved KALGNVG motif thatwas conservative in invertebrates and vertebrate. Moreover，there were 4 conserved domains，Vitellogenin_N，vWFD，DUF1943 and DUF1081，in the predicted protein.The deduced amino acids sequence showed 46％-52％identity with other known decapods Vg by using BLASTP online analysis.Phylogenetic tree rvealed that the Vg formed a clade separated from the decapod species Vg.The qPCR indicated that the Vg mRNA were found in both gonad and hepatopancreas，and had significantly higher expression level of Vg mRNA in ovary than in other tissues（P＜0.05），withoutexpression inmuscle.In the ovary，however，the expression of Vg mRNA was found to be significantly increased from immature stage tomature stage（P＜0.05），then dropped immediately（P＜0.05）.The expression profile in ovary wellwas responding to the annual cycle of vitellogenesis.The findingswill provide basic information for understanding of thewhole function of Vg gene in themantis shrimp.
2016, Vol. 31 
