M orphology and histology of brain and ultrastructure and distribution of lipofuscin in brain ofmantis shrimp Oratosquilla oratoria
FAN Qing-song1,XING Kun1,LIU Hai-ying1,SUN Jing-xian2,CHEN Lei1,LIU Qi1
1.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean University,Dalian 116023,China;2.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China
Abstract: Morphology and histology of brain and ultrastructure and distribution of lipofuscin in brain were investigated inmantis shrimp Oratosquilla oratoria by biopsy,fluorescentmicroscopy and transmission electron microscopy to research the characterization ofmorphology,distribution and ultrastructure of lipofuscin in the brain.The brain was found in the rear of ophthalmic segment and in the small sharp point of cephalothorax,and histologically the rounded cerebral nucleus with diameter of 10-30μm was distributed uniformly in the brain,especially aggregated in the protocerebral bridge cellmass.Under fluorescentmicroscopy,the lipofuscin granules with diameter of 1-3 μm were yellow-brown in color and uniformly distributed in the brain.The transmission electron microscope revealed that lipofuscin was similar to the lysosome in thatboth were covered by single-layered organellesmembrane,and in the granules filled with homogeneous electron-dense contents.The findings indicated that the lipofuscin granules in brain of the mantis shrimp had similar form to other crustaceans and uniform distribution in brain.
范青松, 邢坤, 刘海映, 孙静娴, 陈雷, 刘奇. 口虾蛄脑部褐脂质分布、组织学和超微结构特征研究[J]. 大连海洋大学学报, 2016, 31(2): 147-150.
FAN Qing-song, XING Kun, LIU Hai-ying, SUN Jing-xian, CHEN Lei, LIU Qi. M orphology and histology of brain and ultrastructure and distribution of lipofuscin in brain ofmantis shrimp Oratosquilla oratoria. Journal of Dalian Ocean University, 2016, 31(2): 147-150.