Chromosome karyotype and nuclear DNA content of mantis shrimp Oratosquilla oratoria
LIU Hai-ying1，2，YANG Shuo1，2，YAN Hong-wei3，ZHOU He3，LIU Qi1，2，LIN Yuan4，LI Cheng-jiu4，GUO Liang-yong4
1.Center for Marine Ranching Engineering Science Research of Liaoning，Dalian Ocean University，Dalian 116023，China；2.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province，Dalian Ocean University，Dalian 116023，China；3.College of Fisheries and Life Science，Dalian Ocean University，Dalian 116023，China；4.Fisheries Seedlings Authority of Liaoning Province，Dalian 116015，China
Abstract: The karyotypic pattern was analyzed using colchicine injection method and DNA contents in different tissues were determined using DNA of the chicken blood cell as standard in mantis shrimp Oratosquilla oratoria by a flow cytometer to understand the karyotype，DNA content and genetics content.It was found that there were 88 chromosomes in diploid with 2n=88 in hepatopancreas specimen，with the karyotype formula of 2n=62m+12sm+ 14t，and total chromosome arm number（NF）of 162.The DNA content was significantly different in different tissues（P＜0.05），with the order as：muscle＞testicle＞ovary＞hepatopancreas＞blood，the maximal value（10.43 pg/2c）in muscle and the minimal（8.70 pg/2c）in blood，with an average of 9.61 pg/2c.Moreover，the average value of the mantis shrimp genome size was estimated to be 9398.58 Mbp as 1 pg=978 Mbp.The findings make a first step towards broodstock conservation and complete aquaculture of the mantis shrimp in the future.
刘海映, 杨硕, 闫红伟, 周贺, 刘奇, 林原, 李成久, 郭良勇. 口虾蛄染色体核型分析及DNA含量[J]. 大连海洋大学学报, 2016, 31(1): 1-6.
LIU Hai-ying, YANG Shuo, YAN Hong-wei, ZHOU He, LIU Qi, LIN Yuan, LI Cheng-jiu, GUO Liang-yong. Chromosome karyotype and nuclear DNA content of mantis shrimp Oratosquilla oratoria. Journal of Dalian Ocean University, 2016, 31(1): 1-6.
2016, Vol. 31 
