Cloning and expression profiling of the gsdf gene in red fin puffer Takifugu rubripes
YAN Hong-wei1，TIAN Yu-shun1，JIANG Li-nan1，WANG Lian-shun1，LIU Yang1，LIU Qi2，LIU Sheng-cong3
1.College of Fisheries and Life Science，Dalian Ocean University，Dalian 116023，China；2.Center for Marine Ranching Engineering Science Research of Liaoning，Dalian Ocean University，Dalian 116023，China；3.Dalian Tianzheng Co.Ltd.Dalian 116000，China
Abstract: The full length cDNA of gsdf gene（Trgsdf，GenBank accession No.KR914667）was first cloned in redfin puffer Takifugu rubripeshis using rapid amplification of cDNA ends（RACE）.The results showed that the Trgsdf gene had 1734 bp in length，with 144 bp of5′-untranslated region（UTR），942 bp of3′-UTR and 648 bp of the coding region encoding a 215 amino acids predicted protein.The predicted protein contained a signal peptide（19 amino acid long），a transmembrane region（19 amino acid long），an N-glycosylation site（NST）and a conserverd domain of the TGF-βsuperfamily.A BLAST search against the NCBIprotein database showed that the Trgsdf-encoding protein shared 26％-58％identity with the GSDF proteins found in other fish species.Phylogenetic analysis revealed that the fish GSDF proteins had a clade separated from the othermembers of the TGF-βsuperfamily.Moreover，the Trgsdf-encoding protein showed a higher similarity with themedaka Oryzias latipes GSDF and the protogynous wrasse Halichoeres trimaculatusgsdf as compared with the GSDF in other species.The expression pattern of Trgsdf in various tissues by RT-PCR（reverse transcription polymerase chain reaction）showed that therewas Trgsdf transcripts in both female and male redfin puffer，with a high expression level in ovary and testis，low expression level in skin andmuscle and withoutexpression in other tissues.Relative real-time PCR showed that there was significantly higher expression level of Trgsdf in testis than that in ovary（P＜0.05）.The findings suggest that GSDF might be involved in the development of gonad（especially in testis differention and development）in redfin puffer.
闫红伟, 田雨顺, 姜丽楠, 王连顺, 刘洋, 刘奇, 刘圣聪. 红鳍东方鲀gsdf基因的克隆及表达模式分析[J]. 大连海洋大学学报, 2016, 31(2): 140-146.
YAN Hong-wei, TIAN Yu-shun, JIANG Li-nan, WANG Lian-shun, LIU Yang, LIU Qi, LIU Sheng-cong. Cloning and expression profiling of the gsdf gene in red fin puffer Takifugu rubripes. Journal of Dalian Ocean University, 2016, 31(2): 140-146.
2016, Vol. 31 
