Expression of genes related to temperature in sea cucumber Apostichopus japonicas under the stimulated winter and spring temperature in different coasts
BAI Xue-qiu, DING Jun, CAO Xue-shun, LI Cheng-ze, CHANG Ya-qing
Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
Abstract: The genes related to temperature including hsp70, gp96, UQCRFS1, ferritin, lysozyme, mtLSU, and proeintl(2)efl selected from the subtracted cDNA library were expressed in body wall, intestine and respiratory tree of sea cucumber Apostichopus japonicas using quantitative Real-time PCR under the simulated sea water temperature in Russian coastal area, Dalian coastal area, and Fujian coastal area from December to April in a laboratory. The expression of hsp70 gene was found to be not significant difference in the same tissue in the three temperature groups, but there was significant differences in different tissues at the same conditions. There was higher expression of gp96 gene in the simulation of Fujian coastal area in winter and spring group than in the other two groups, significantly higher relative expression levels in respiratory tree than in intestine and the body wall in the simulation of Fujian coastal area in winter and spring group (P<0.05). The expression of UQCRFS1 gene was higher in the simulation of Russian coastal area in winter and spring groups than in the other two groups. No same ferritin gene expression patterns and relative expression levels was observed in the three temperature groups. The maximal relative expression levels of both lysozyme and proeintl (2)efl genes were found in the respiratory tree, significantly higher than in the intestine and body wall in the three groups (P<0.05). There were essentially the same mtLSU gene expression patterns in the three groups, significantly higher relative expression levels in the body wall than that in the intestine and respiratory tree (P<0.05).
白雪秋, 丁君, 曹学顺, 李成泽, 常亚青. 刺参温度相关基因在模拟不同海域冬春季水温条件下的表达研究[J]. 大连海洋大学学报, 2015, 30(1): 18-24.
BAI Xue-qiu, DING Jun, CAO Xue-shun, LI Cheng-ze, CHANG Ya-qing. Expression of genes related to temperature in sea cucumber Apostichopus japonicas under the stimulated winter and spring temperature in different coasts. Journal of Dalian Ocean University, 2015, 30(1): 18-24.
2015, Vol. 30 
