Abstract: In order to investigate the mechanism of the response of scalloped spiny lobster Panulirushomarus to nitrite stress, the transcriptomics were comparatively analyzed in hepatopancreas of scalloped spiny lobster with body weight of (39.00±5.31)g exposed to under normal conditions <0.01 mg/L, control group)and to 80 mg/L nitrite stress prepared by NaNO2 for 7 d by a transcriptomic sequencing approach. The results showed that 264 differentially related genes were screened, with up-regulated 86 and down-regulated 178. GO functional annotation results showed that the annotated differential genes were primarily involved in functions including binding, catalysis and metabolism, and KEGG pathway enrichment analysis showed that the differentially expressed genes were significantly enriched in focal adhesion, leukocyte transendothelial migration, insulin resistance, tight junctions, and starch and sucrose metabolism pathways. Nine differential genes were randomly selected for qRT-PCR validation, and the fluorescence quantification results were found to be in general agreement with the sequencing results. The findings will provide substantial reference for the research of gene functions in energy metabolism, growth and immunity in scalloped spiny lobster under nitrite stress.
2022, Vol. 37 
