cDNA cloning and expression distribution of Wnt9 gene in adult tissues and embryonic development in sea cucumber Apostichopus japonicas
MA Deyou,YU Lianlian,LIN Weigang,WANG Chengliang,DING Jun,CHANG Yaqing
College of Fisheries and Life Science,Key Laboratory of Mariculture &Stock Enhancement in North China’s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China
Abstract: In order to understand of the regulatory roles of Wnt9 gene in early development of sea cucumber Apostichopusjaponicus,the molecular characteristic and dynamic expression pattern of Wnt9 gene were investigated through RACE,real-time PCR and whole mount in situ hybridization techniques.It was found that the full-length cDNA of Wnt9 gene in sea sucumber was 1 603 bp,with an 1 104 bp open reading frame encoding 367 amino acid residues containing a conserved Wnt1 domain.Wnt9 shared common features of Wnt family including N-glycosylation sites and conserved cysteine sequences.Phylogenetic analysis revealed that the deduced amino acid sequence of Wnt9 in sea sucumber was highly similar to that of Wnt9a and Wnt9a like from the genetically close species in echinoderm.qRT-PCR analysis indicated that Wnt9 gene was differentially expressed in adult tissues of sea sucumber,with the maximum in body wall (P<0.05),and the minimum in gonad (P<0.05).Wnt9 was expressed throughout the embryonic development of sea sucumber,and its expression levels were increased significantly at the doliolaria stage and peaked at the juvenile stage (P<0.05).Further investigation using whole mount in situ hybridization showed that Wnt9 mRNA positive signals were observed in doliolaria,and enhanced in juveniles,indicating that Wnt9 gene has effect on the embryonic growth and development,especially postmetamorphic stage in this important economic species.The findings lay a foundation for exploring the formation of the head-to-tail axis and the morphological remodeling during metamorphosis of sea cucumber.
马得友, 于莲莲, 林威港, 汪成粱, 丁君, 常亚青. 刺参Wnt9基因克隆、组织分布及在胚胎发育中的表达分析[J]. 大连海洋大学学报, 2022, 37(3): 394-402.
MA Deyou, YU Lianlian, LIN Weigang, WANG Chengliang, DING Jun, CHANG Yaqing. cDNA cloning and expression distribution of Wnt9 gene in adult tissues and embryonic development in sea cucumber Apostichopus japonicas. Journal of Dalian Ocean University, 2022, 37(3): 394-402.
2022, Vol. 37 
