Influence of inbreeding in 9 successive generations of a fam ily on metamorphosis and morphology of sw imm ing crab Portunus trituberculatus
LIU Xian-xi1，2，3，REN Xian-yun2，3，GAO Bao-quan2，3，LIU Ping2，3，LIJian2，3
1.College of Fisheries and Life Science，Dalian Ocean University，Dalian 116023，China；2.Key Laboratory of Sustainable Development of Marine Fisheries，Ministry of Agriculture，Yellow Sea Institute of Fisheries，Chinese Academy of Fishery Sciences，Qingdao266071，China；3.Functional Laboratory of Marine Fisheries Science and Food Production Process，State Laboratory of Qingdao Marine Science and Technology，Qingdao266235，China
Abstract: Nine generations of swimming crab Portunus trituberculatus within a same family（F1-F9）were systematically bred to reveal the influence of inbreeding on larval development and morphology in swimming crab via breeding and growth with ANOVA statistical analysis on one direction.The results showed that there was decline in survival rate（metamorphosis）at each stage from zoeaⅠ（Z1）tomegalopa（M）in each generation，74.87％from Z1 to Z2，73.50％from Z2 to Z3，59.17％from Z3 to Z4，and 41.96％from Z4 to M，with theminimal survival in F9，though no linear relationship between survival and each inbreeding generation from F1to F9.Themorphometrical valueswere shown to be declined from F3to F9，theminimal value in F9，significant from the other generations in the 80 days.However，the uniformity analysis of150 days old swimming crab revealed that the F9had variation coefficient of 7.636 5 in carapace width，7.559 1 in carapace length and 8.328 2 in body height，the least variation coefficient in all the generations.There were influences of various generations on larvalmetamorphosis，larval size and growth characteristics of swimming crab，showing inbreeding depression phenomenon.During the production season，compared with the F1inbreeding generation by increasing the inbreeding coefficient，except for F2，the estimated inbreeding depression coefficient was-6.12％--21.95％in overall carapace width，-1.85％--16.36％in carapace width，-10.08％--27.66％in carapace length，-9.39％--22.70％in body height，-13.39％--47.99％in body weight，and-9.03％--45.13％in larval yield，with higher depression coefficient in body weight and larval production than in other morphometrical values.The findings suggest that inbreeding should be avoided in crab breeding programs and that have a purpose in planned selective breeding.
刘贤曦, 任宪云, 高保全, 刘萍, 李健. 连续九代家系内自交对三疣梭子蟹幼体变态和形态性状的影响[J]. 大连海洋大学学报, 2016, 31(5): 469-476.
LIU Xian-xi, REN Xian-yun, GAO Bao-quan, LIU Ping, LIJian, . Influence of inbreeding in 9 successive generations of a fam ily on metamorphosis and morphology of sw imm ing crab Portunus trituberculatus. Journal of Dalian Ocean University, 2016, 31(5): 469-476.
2016, Vol. 31 
