Abstract: The effects of temperature（8 ℃ and 34 ℃） stress for 12 hours on serum immunity parameters including the content of hemocyanin,and activities of superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT）,hydrolase（acid phosphatase ACP,and alkaline phosphatase AKP） were studied in swimming crab Portunus trituberculatus between the second generation of candidate disease-resistance stock（G2S） screened by the common stock（RS）.Statistically,the significant effect of temperature stress was observed between two groups of G2S and RS during 12 hours.The content of hemocyanin was found to be increased in the groups of G2S and RS,a peak value at 3 h（P〈0.05）,and then to be decreased significantly after 5h and constant during 7-12 h.In addition,there were significant decrease in SOD and AKP activities in the two groups during 3 h（P〈0.05）,then stable after 3h.POD,CAT and ACP activities were also increased to a peak value at different times.There were higher content of hemocyanin and serum enzyme activities in G2S than those in the RS at both 0 h and stress time（P〈0.05）.These results revealed that the rapid increment in temperature could result in metabolic maladjustment of free radicals and have profound effects on physiological and immune functions,and that G2S had the higher immune ability compared with RS.
2010, Vol. 25 
