Abstract: Activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were determined in rainbow troutOncorhynchusmykisswith body weight of (240 g±18 g)exposed to low (6 ℃) and high temperature(24 ℃) by a BS-180 biochemical analyzer, and levels of serum insulinlike growth factor-1 (IGF-1) and the expression ofGHR1,IGF-1 andIGF-2 in liver were detected by radioimmunoassay and by real-time quantification. The activities of serum AST and ALT were shown to be increased first and then decreased, the maximum at 6 h and significantly higher in the lower temperature group for ALT even at 0 h(P<0.05), finally being reduced to the level in the control group. The ALP activity was significantly decreased at 0 h (P<0.05), and then maintained low level in low temperature group, while the ALP activity was decreased slightly in high temperature group. The serumIGF-1 level was found increase at 0 h and then recovery to the level in the control group at low temperature exposure. In the high temperature exposure, however, the serumIGF-1 level was kept at low level, without significant difference (P>0.05). The expression ofIGF-1 andIGF-2 genes was shown to be decreased firstly and then increased, the minimum at 12 h, significantly lower forIGF-2 gene. The findings revealed that acute temperature led to harmful liver and to increase in AST and ALT activities and to decrease in ALP activity; meanwhile serumIGF-1 level and the expression ofGHR1,IGF-1 andIGF-2 also were decreased, indicating that acute temperature may affect the expression of growth related genes.
2014, Vol. 29 
