Effects of Clostridium butyricum and Bacillus coagulans on growth performance, hepatic function and intestinal microbiota in rainbow trout Oncorhynchus mykiss
FAN Ying1, YE Hai-bin1, WANG Xiao-lu1, LI Le1, WANG Yong-qiang2, DIAO Jing1
1.Shandong Key Laboratory of Disease Control in Mariculture, Marine Biology Institute of Shandong Province, Qingdao 266104, China; 2.Rizhao Wanzefeng Fisheries Limited Company, Rizhao 250000, China
Abstract: Rainbow trout Oncorhynchusmykiss with average body weight of (73.0±2.0) g were reared in aquarium (80 cm×50 cm×60 cm) and fed basal diet as control group (T0) at stocking density of 20 individuals per aquarium, and the basal diet supplemented with Clostridiumbutyricum(7.35×109 CFU/kg, T1), Bacilluscoagulans(2.4×109 CFU/kg, T2), and Clostridiumbutyricum+ Bacilluscoagulans (7.35×109 CFU/kg+2.4×109 CFU/kg, T3) for 40 days in order to investigate the effects of Clostridiumbutyricum (CGMCC1.336) and Bacilluscoagulans (CGMCC1.3220) on growth performance, hepatic function and intestinal microbiota of rainbow trout with 3 replicates. The specific growth rate (SGR) were found to be significantly higher in the fish fed the diets containing Clostridiumbutyricum and Bacilluscoagulans (P<0.05), with the maximal SGR in groups T1 and T3 (1.41%/d) (P<0.05), and with food conversion ratio (FCR) of 1.08 and 1.10, respectively (P<0.05). There was significantly higher crude protein in groups T1 and T3 than that in the control (P<0.05), without significant differences in the crude ash content and moisture in all groups(P>0.05). There were significantly lower activities of glutamic oxaloacetic transaminase (GOT), and γ-glutamyl transpeptidase(γ-GT), total bilirubin content (TBiL) and hepato-somatic index in live in T3 group(P<0.05), with significantly higher activity of cholinesterase (CHE) in groups T2 and T3(P<0.05). The indices of Chao1, Simpson and Shannon were increased in T3(P<0.05), meanwhile the phylum level of bacterial community was changed, with significant decrease in proportion of Proteobacteria in T2 and T3, and with increase in proportions of Firmicutes, Bacteroidetes and Fusobacteria in T1 and T3. The findings indicate that dietary C.butyricum and B.coagulans leads to promote the growth performance and body composition, to protect the hepatic function, and to optimize the intestinal microbiota in rainbow trout.
樊英, 叶海斌, 王晓璐, 李乐, 王勇强, 刁菁. 丁酸梭菌和凝结芽孢杆菌对虹鳟生长性能、肝功能及肠道菌群的影响[J]. 大连海洋大学学报, 2019, 34(2): 198-203.
FAN Ying, YE Hai-bin, WANG Xiao-lu, LI Le, WANG Yong-qiang, DIAO Jing. Effects of Clostridium butyricum and Bacillus coagulans on growth performance, hepatic function and intestinal microbiota in rainbow trout Oncorhynchus mykiss. Journal of Dalian Ocean University, 2019, 34(2): 198-203.
2019, Vol. 34 
