Effects of abrupt and gradual decreases in water tem perature on blood physiological and biochem ical parameters in dusty rabbit fish Siganus fuscescens
NING Jun-hao1,2,QIN Yu-bo3,HU Lun-chao1,ZHANGWei-jie1,LILie1,CHANG Ya-qing1,SONG Jian1
1.Key Laboratory of Mariculture&Stock Enhancement in North China蒺s Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China; 2.Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao266071,China;3.Liaoning Prov-ince Marine Environmental Forecasting Center,Shenyang 110001,China
Abstract: In this study,hematology and serum biochemical parameterswere investigated in dusty rabbit fish Siga-nus fuscescens with body weight of(37.12依5.60)g exposed to abrupt(24益in control group--20益--16益--8益,sampled in 1 h acclimatization)and gradual(24益in control group--20益--8益at a rate of1益/3 h, sampled in 1 h acclimatization)decreases in water temperature.The results showed that the number of red blood count(RBC),hemoglobin(HGB),and hematocrit(Hct)in hematology were significantly decreased at8益,in the abrupt decrease temperature groups,with first increase-then decrease in activities of glutamyl-transpeptidase (ALT)and alkaline phosphatase(AKP).Therewas significantly higher ALT activity than that in control group ex-cept in group 20益,with significantly higher AKP activity at 20益(P<0.05).Glutamate-oxaloacetate(AST) activity was shown to be a fluctuation tendency,significant increase from the initial level(P<0.05).Glucose (GLU)concentration in serum was significantly increased at 8益,while significantly lower in the other tempera-ture treatments than the initial level(P<0.05).There was significantly lower concentrations of cholesterol (CHOL),total protein(TP)and albumin(ALB)in the other temperature except for 20益than those in the con-trol group.The triglyceride(TG)and creatinine(CREA)concentrationswere significantly increased at20益,sig-nificantly lower TG at the other temperature than the initial level(P<0.05).In gradual decrease in water tempera-ture,however,RBC and HGB were decreased significantly(P<0.05),without significant differences in the other hematology parameters(P>0.05).There were significantly higher ALT activities at12益than those in the control group,and significantly higher AST activities at 20益and 12益than those in control group.It was found that AKP activitieswere significantly increased in all groups except for at16益(P<0.05).GLU concentration was sig-nificantly increased at8益,and CHOL concentration significantly decreased at16益and 12益(P<0.05).The concentration of TG,TP and CREA showed a increase-decrease trend,the TP concentration being not recovered to the initial level.ALB concentration was significantly decreased compared to the initial level except for at20益(P< 0.05).The findings suggest that dusty rabbit fish have lower limit temperature for survival at12益,and lethal low temperature of 8-9益.
宁军号, 秦宇博, 胡伦超, 张伟杰, 李磊, 常亚青, 宋坚. 水温骤降和缓降胁迫对褐篮子鱼血液生理生化指标的影响[J]. 大连海洋大学学报, 2017, 32(3): 294-301.
NING Jun-hao, QIN Yu-bo, HU Lun-chao, ZHANGWei-jie, LILie, CHANG Ya-qing, SONG Jian. Effects of abrupt and gradual decreases in water tem perature on blood physiological and biochem ical parameters in dusty rabbit fish Siganus fuscescens. Journal of Dalian Ocean University, 2017, 32(3): 294-301.
2017, Vol. 32 
