Effects of dietary glutathione on growth and anti－oxidative capability of rainbow trout Oncorhynchus mykiss
WANG Yi1， SUN Guo－xiang2、3， LIU Ying1， YANG Xiu－liang3， ZHANG Xu3， WANG Shun－kui4
1.Aquacultural Engineering Research ＆ Development Center， Dalian Ocean University， Dalian 116023， China； 2.Institute of Oceanology， Chinese A-cademy of Sciences， Qingdao 266000， China； 3.Shandong Jincheng Biological Pharmaceutical Company Limited， Zibo 255000， China； 4.Shandong Oriental Ocean Company Limited， Yantai 264003， China
Abstract: The rainbow trout Oncorhynchus mykiss with initial body weight of(718.3±36.3)g were reared in a recycling water system and fed diets containing glutathione(GSH)at a rate of 0，100，200，400 and 600 mg/kg at water temperature of(15.0±1.0)℃ for 8 weeks to investigate the effects of dietary glutathione on the growth performance and antioxidant ability and resistance to nitrite exposure in rainbow trout.The results showed that average weight gain rate(WGR)，specific growth rate(SGR)and food intake(FI)were found to be elevated first and then declined with increase in dietary SGR level，the maximum in the fish fed the diet containing 200 mg/kg of GSH and significantly higher than those in other groups(p＜0.05).There was significantly lower food conversion ratio in 200 mg/kg group than in other groups(p＜0.05)， with the minimal mortality rate(4.04%).The gill filament and serum superoxide dismutase(SOD)activities in GSH－fed fish were higher than those in the control group，significantly higher serum and hepatic SOD activities in 200 mg/kg GSH groups.The gill filament，serum and hepatic catalase(CAT)activities were higher in GSH－fed fish than those in the control group， increase first and then decrease with elevated dietary GSH level， significantly higher serum and hepatic CAT activities in 200 mg/kg GSH groups(p＜0.05).There were significantly lower malondialdehyde(MDA)levels in serum，liver and gill filament in GSH－fed fish than those in the control group， without significant difference(P＞0.05)， and the minimum in the fish fed the diet containing GSH at a rate of 200 mg/kg.The findings indicate that supplementation of suitable amount of glutathione leads to improve the growth and antioxidant capacity of rainbow trout.
2017, Vol. 32 
