1.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;2.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;3.Chinese Academy of Fishery Sciences,Beijing 100039,China
Abstract: Activities of protease, amylase and lipase were analyzed and compared in stomach, intestine and pyloric caecum in female sterletAcipenserruthenusbroodstock with different developmental phases (Ⅱ-Ⅴ). The results showed that the maximal protease activity was observed in the broodstock with Ⅱ phase ovary, and that higher protease activity in the broodstock with Ⅲ phase ovary. The amylase activity was found to be higher in the broodstock with Ⅴ phase ovary, and the lipase activity to be higher in broodstock with Ⅴ and Ⅱ phase ovaries. The minimal activities of protease, amylase and lipase were observed in the broodstock with Ⅳ phase ovary, and in the broodstock with different gonadal developmental phases higher protease activity was observed in pyloric caecum and hindgut, followed by in the stomach and midgut. The maximal amylase activity, however, was found in hindgut, followed by in the stomach, pyloric caecum, foregut and hindgut. The maximal lipase activity was observed in the pyloric caecum, and higher lipase activity was found in the intestine and stomach. The findings indicated that the sterlet broodstock with Ⅱ and Ⅲ phase ovary required more dietary protein and carbohydrate, while the sterlet broodstock with Ⅴ or Ⅱphase ovary needed more lipid. During the whole gonadal development, the sterlet broodstock required more dietary lipid.
2014, Vol. 29 
