1.Key Lab of Tropical & Subtropical Fish Breeding & Cultivation, Agriculture Ministry, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China；2.Department of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
Abstract: Largemouth bassMicropterussalmoideswith mean body weight of 40 g were fed frozen fresh fish (as control), formulated diet A (fat level 8.2%) and formulated diet B (fat level 14.5%) for 3 months, and mRNA expression levels of two lipoprotein lipases (LPL), LPLtype1 and LPLtype2, were detected in livers of the largemouth bass. The results showed that there was significantly higher average body weight gain in the fish fed the frozen trash fish than the fish fed formulated diet A and formulated diet B (P<0.05). The fish fed formulated diet B had significant differences in hepatic expression levels of LPLtype1 and LPLtype 2 mRNA from the largemouth bass fed formulated diet A and the fish fed the frozen fresh fish (P<0.05), with the higher expression levels of LPLtype1 and LPLtype2 mRNA in the fish fed the higher dietary fat level. The findings indicate that LPLs play an important role in hydrolysis of the extra fat in hepato cytes. There are more precise function divisions between LPLtype1 and LPLtype 2 genes, and LPLtype 2 is more susceptible to dietary fat concentration than LPLtype 1 does, showing more important function in lipoprotein metabolism hepatocytes.
2014, Vol. 29 
