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大连海洋大学学报  2020, Vol. 35 Issue (4): 522-527    DOI: 10.16535/j.cnki.dlhyxb.2019-337
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大气CO2浓度升高对繁茂膜海绵硅酶基因表达及胁迫后滤食新月菱形藻能力的影响
李东子1，王啸宇2，徐铭2，沈思思1，丁小涵1， 郭君宁1，李俊成1，张世杰1，付晚涛1、3*
1.大连海洋大学 海洋科技与环境学院，辽宁 大连 116023; 2.大连海洋大学 水产与生命学院，辽宁 大连 116023;3.辽宁省高校近岸海洋环境科学与技术重点实验室，辽宁 大连 116023
Effects of elevated CO2 concentration on silicatein gene expression and ability to filtering diatom Natzchia closterum of marine sponge Hymeniacidon perlevis
LI Dongzi1, WANG Xiaoyu2, XU Ming2, SHEN Sisi1, DING Xiaohan1, GUO Junning1, LI Juncheng1, ZHANG Shijie1, FU Wantao1,3*
1.College of Marine Science and Environment，Dalian Ocean University, Dalian 116023, China; 2.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 3.Key Laboratory of Offshore Marine Environmental Science and Technology of Liaoning Province’s University, Dalian 116023, China
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摘要 为探究大气CO2浓度升高对海洋硅质生物的影响，以繁茂膜海绵Hymeniacidon perlevis为模式生物，在实验室条件下模拟大气CO2浓度升高(500、750、1000 mg/L)对海绵硅酶基因相对表达的影响，以及胁迫48 h后在当前大气CO2(390 mg/L)下恢复1 h时海绵滤食新月菱形藻Natzchia closterum能力的变化。结果表明：500 mg/L 大气CO2胁迫12、24、48 h时，海绵硅酶基因表达量分别比对照组(390 mg/L CO2)提高24.8%、14.8%、19.2%，而750、1000 mg/L大气CO2胁迫12、24、48 h时，海绵硅酶基因表达量分别比对照组降低40.0%、71.9%、82.3%(750 mg/L CO2)，55.2%、83.6%、80.8%(1000 mg/L CO2)；经高浓度大气CO2胁迫48 h再恢复1 h时，24 h内对照组海绵块滤食微藻的平均效率和平均清除率分别为198.2×104cells/(h·g)和4.09 mL/(h·g)，而500、750、1000 mg/L大气CO2浓度组海绵滤食微藻的平均效率与平均清除率分别为对照组的104.8%、96.6%(500 mg/L CO2)，58.5%、65.3%(750 mg/L CO2)，30.8%、20.0%(1000 mg/L CO2)。研究表明，大气CO2浓度升高至750 mg/L以上时明显抑制了海绵硅酶基因表达，并导致海绵滤食微藻能力大幅下降。
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李东子
王啸宇
徐铭
沈思思
丁小涵
郭君宁
李俊成
张世杰
付晚涛
关键词:  大气CO2浓度  繁茂膜海绵  硅酶基因表达  滤食能力    
Abstract: Marine sponge Hymeniacidon perlevis was held in a 25 L inter-tidal zone ecological environment simulation system and exposed to CO2 level of 500, 750, and 1000 mg/L prepared by an air bag at a rate of 0 (control group) and 25 g of sponge to investigate effect of elevated CO2 concentration on silicatein gene expression in marine sponge. Then the marine sponge recovered for 1 h from the stress of elevated atmospheric CO2 concentration were reared in a 500 mL Erlenmeyer flask containing diatom Natzchia closterum at (105.5±8.6)×106 cells/mL at 20 ℃ and light intensity of 80 μmol /(m2·s) to probe into effect of high CO2 level on ability to filter the diatom of sponge. The results showed that there were 24.8%, 14.8% and 19.2% higher silicatein gene expression levels in the marine sponge exposed to atmospheric CO2 concentration of 500 mg/L at 12, 24 and 48 h than those in the control group (CO2 concentration of 390 mg/L), 40.0%, 71.9%, and 82.3% lower in the marine sponge exposed to atmospheric CO2 concentration of 750 mg/L than those in the control group and 55.2%, 83.6%, and 80.8% lower in the marine sponge exposed to atmospheric CO2 concentration of 1000 mg/L CO2 than those in the control group. The marine sponge recovered for 1 h from the 24 h stress of elevated atmospheric CO2 concentration in the control group had average filtering rates (FR) of 198.2×104 cells /(h·g)and clearance rates (CR) of 4.09 mL/(h·g), as 104.8% and 96.6% in 500 mg/L CO2 group, 58.5% and 65.3% in 750 mg/L CO2, and 30.8% and 20.0% in 1000 mg/L CO2 in as those the control group within 24 h. The findings demonstrated that above 750 mg/L CO2 concentration led to markedly inhibit silicatein gene expression in the marine sponge and to result in decrease in FR and CR of the sponge.
Key words:  atmospheric CO2 level    marine sponge Hymeniacidon perlevis    silicatein gene expression    filtering ability
               出版日期:  2020-10-09      发布日期:  2020-10-09      期的出版日期:  2020-10-09
中图分类号:  Q812  
基金资助: “十二五”国家科技支撑计划项目(2015BAD13B05)；大连海洋大学2018年大学生创新创业训练计划项目(201810158104)
引用本文:    
李东子, 王啸宇, 徐铭, 沈思思, 丁小涵, 郭君宁, 李俊成, 张世杰, 付晚涛. 大气CO2浓度升高对繁茂膜海绵硅酶基因表达及胁迫后滤食新月菱形藻能力的影响[J]. 大连海洋大学学报, 2020, 35(4): 522-527.
LI Dongzi, WANG Xiaoyu, XU Ming, SHEN Sisi, DING Xiaohan, GUO Junning, LI Juncheng, ZHANG Shijie, FU Wantao, . Effects of elevated CO2 concentration on silicatein gene expression and ability to filtering diatom Natzchia closterum of marine sponge Hymeniacidon perlevis. Journal of Dalian Ocean University, 2020, 35(4): 522-527.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.16535/j.cnki.dlhyxb.2019-337  或          https://xuebao.dlou.edu.cn/CN/Y2020/V35/I4/522
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