Expression comparisons of heat shock proteins gene in gills of zebrafish exposed to both normoxia and hypoxia
DI Zhi-chao1, ZHOU Tao1, XU Qiang-hua1,2,3,4
1.College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China; 2.Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306,China; 3.National Distant-water Fisheries Engineering Research Center, Shanghai 201306, China; 4.Collaborative Innovation Center for Distant-water Fisheries, Shanghai 201306, China
Abstract: The transcriptome analysis of heat shock proteins (HSPs) was conducted and differential expressed genes were screened in gills of zebrafishDaniorerio exposed to hypoxia and normoxia for two weeks in order to research the biological functions of zebrafish responding to the hypoxia stresses. Among the compared gill transcriptome data, 80 heat shock protein family members were acquired, and 28 differential expressed HSP genes were screened in the hypoxic and normoxic gills. It was found that 16 and 12 genes showed significantly up-regulated and down-regulated expressions(P<0.05), respectively,Hsp47 being the most up-regulated HSP family members under the hypoxia condition, andHsp90ab1 being the only up-regulated macromolecule HSP member. Gene expressions ofHsp47 andHsp90ab1 were detected in different tissues under hypoxia and normoxia by qRT-PCR analysis, indicating thatHsp47 had significantly up-regulated expressions in the gill and muscle tissues(P<0.01), and thatHsp90ab1 showed significantly up-regulated expressions in the gill, skin and muscle tissues under hypoxia stress(P<0.05 orP<0.01). The western blotting analysis revealed that HSP47 and HSP90ab1 proteins showed significantly up-regulated expressions in gills of zebrafish exposed to different hypoxia stresses(P<0.01). It is concluded that HSP47 and HSP90ab1 play important roles in hypoxia response and are the most important HSP family members during the hypoxia response.
狄治朝, 周涛, 许强华. 低氧胁迫与常氧条件下斑马鱼鳃中热休克蛋白基因家族的表达差异比较[J]. 大连海洋大学学报, 2018, 33(6): 690-695.
DI Zhi-chao, ZHOU Tao, XU Qiang-hua, . Expression comparisons of heat shock proteins gene in gills of zebrafish exposed to both normoxia and hypoxia. Journal of Dalian Ocean University, 2018, 33(6): 690-695.
2018, Vol. 33 
