Abstract: In order to study effects of compound probiotics on the delayed reproductive performance, physiological indices and larval survival rate in largemouth bass Micropterussalmoides, 150 pairs of broodstock with body mass of 550-665 g in female and 500-573 g in male were randomly reared in 15 tanks of each 5.0 m×2.7 m×1.0 m, and fed 5 diets supplemented with 0, 0.1%, 0.3%, 0.5% and 0.7% of compound probiotics in the basic diet with 3 replicates at water temperature of below 17.5 ℃ for 30 days in early July. Then, 3 d after rearing, the largemouth bass broodstocks were induced to spawn at water temperature warming to 21.5-22.5 ℃ by hormone injection. The results showed that the gonado-somatic index (GSI), fecundity and relative fecundity of female were increased gradually with the increase in compound probiotics dose, with the peak in 0.5% group, without significant difference in GSI, fecundity and relative fecundity among the other test groups (P>0.05). There were higher fertilization rate, hatching rate and larval survival rate in first batch in 0.3% and 0.5% groups than those in other groups, with significantly higher survival rate in 0.5% group than that in control group and 0.1% group(P<0.05). There was gradual increase in activities of alkaline phosphatase (AKP) and acid phosphatase (ACP) in liver of female largemouth bass in groups before spawning, and higher than those after spawning(P<0.05), with the peak AKP and ACP activity in the 0.5% group, without significant difference in the activity of total superoxide dismutase (T-SOD) in liver of female fish among different groups before spawning(P>0.05), significantly higher activity of T-SOD in plasma in 0.3% and 0.5% groups than those in other groups (P<0.05). The significant improvement of the larval survival rate and enhancement of the activities of AKP and ACP in the liver and T-SOD activity in plasma were observed in the female fish in 0.5% group. The finding provides theoretical basis for the application of compound probiotics in the delayed breeding of largemouth bass.
王赛赛, 朱莉飞, 李荣妮, 史东杰, 孙砚胜, 张欣. 复合益生菌对大口黑鲈延后繁殖性能、生理指标及仔鱼成活率的影响[J]. 大连海洋大学学报, 2021, 36(6): 959-965.
WANG Saisai, ZHU Lifei, LI Rongni, SHI Dongjie, SUN Yansheng, ZHANG Xin. Effects of compound probiotics on reproductive performance, physiological indices and larval survival rate in largemouth bass Micropterus salmoides. Journal of Dalian Ocean University, 2021, 36(6): 959-965.