Abstract: The effect of chitosan and its constituents oligosaccharides(chitosan oligosaccharides)and monosaccharides (glucosamine and N-acetylglucosamine)in the matrix of aquatic products on digestion of DNA by pepsin was investigated to understand theory of pepsin digestion of DNA and to further explore its mechanism. The effects of different mass ratios of sugar to DNA on pepsin digestion of salmon sperm DNA and λDNA were evaluated in vitro by using simulated gastric juice. The electrophoresis showed that chitosan inhibited the degradation of DNA at mass ratio of chitosan to nucleic acid = 0.5∶1 for 2 hours at physiological pH, the obvious inhibition at mass ratio of oligochitosan to nucleic acid = 5∶1. However, there was no inhibitory effect of glucosamine and N-acetylglucamine on digestion within the range of 1∶1-240∶1 mass ratio of DNA to glucosamine. It is concluded that chitosan and oligochitosan can inhibit the digestion of DNA by pepsin due to the interaction of positively charged sugars with negatively charged DNA, or due to the binding of sugars with pepsin as to protect DNA from digestion.
张金勇, 高养春, Amir Shah Ruddin Md Sah, Aileen TAN Shau-Hwai, Norlaila Binti Mohd Zanuri, 李宏俊. 基于高通量测序和形态学鉴定的马来西亚马塘红树林区浮游动物多样性分析[J]. 大连海洋大学学报, 2021, 36(6): 910-919.
2019, Vol. 34 
