Application of microsatellite markers in parental apolegamy of redfin puffer Takifugu rubripes
LIU Yong-xin1, LIU Yi2, ZHOU Qin3, ZHANG Hong-tao3
1.Chinese Academy of Fishery Sciences, Beijing 100141, China; 2.Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 3.Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China
Abstract: A family mating plan of redfin puffer Takifugu rubripes was established by parental apolegamy assisted by microsatellite markers and by analysis of genetic variations and distances of parental populations, and genetic evaluation was carried out in two populations of the redfin puffer by a set of 34 microsatellite markers. The results showed that there were significantly higher allele number (6.647 0) and Nei’s gene diversity index (0.711 5) in population A than those (allele number 4.647 1 and Nei’s gene diversity index 0.646 1) in population B, significant genetic differences between the two populations (P<0.05). The genetic differentiation coefficient GST of 0.050 7 and Nm of 4.679 6 indicated that there were inferior differentiation and some degree of gene exchange among the redfin puffer populations. AMOVA revealed that there was 78.49% variation within the population while there was 21.51% variation between the two populations. The UPGMA tree established by genetic distance indicated that the genetic distance (GD) among individuals within the population was varied from 0.11 to 0.82. All individuals were divided into different branches on basis of genetic distance magnitude. Each genetic parameter demonstrated that there were genetic variations to some extent in experimental population and distant relatives among individuals. The breeding schedule can be made according to the genetic distances among individuals, and then families can be produced for selective breeding. The findings indicate that the method is feasible for establishment of families in redfin puffer with microsatellite marker information.
2015, Vol. 30 
