Genetic variability and relationship between rock scallop Crassadoma gigantea and three other scallops based
on sequence of mtDNA Cyt b gene
LIAO De－jie1， TONG Jin－gou2， CAO Shan－mao1， ZHOU Ying2，3，YU Xiao－mu2， FU Bei－de2， LIU Yang1， WANG Xiao1
1.Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province， Dalian Ocean University， Dalian 116023， China； 2.State Key Laboratory of Freshwater Ecology and Biotechnology， Institute of Hydrobiology， Chinese Academy of Sciences， Wuhan 430072， China； 3.University of Chinese Academy of Sciences， Beijing 100049， China
Abstract: The sequences of Cyt b gene were amplified by PCR and sequenced to obtain the whole sequences of Cyt b gene in 10 individuals sampled randomly from four scallop species including rock scallop Crassadoma gigantea，bay scallop Argopecten irradians，Zhikong scallop Chlamys farreri and yesso scallop Patinopecten yessoensis to understand the genetic variation and the relationships among the four scallop species.Then，the related analysis of sequence characterization， genetic diversity， genetic distance and phylogenetic evolution were analyzed by related software.The results showed that， the sequences of Cyt b gene in Zhikong scallop， rock scallop， bay scallop and yesso scallop were shown more than 99%homologous to the corresponding sequences in GenBank，with haplotype number of 3－4， polymorphic sites of 2－15， haplotype diversity of 0.545－0.800， nucleotide diversity of 0.001 00－0.005 72 and average nucleotide difference of 0.788－6.545， indicating that the maximal genetic diversity(0.429)between rock scallop and bay scallop and the minimal(0.255)between rock scallop and yesso scallop.The phylogeny trees further confirmed that there was close genetic relationship between rock scallop and yesso scallop.The finding provides basic information for future research on species introduction and intensive culture，germplasm resources management，and genetics and breeding in rock scallop.
廖德杰, 童金苟, 曹善茂, 周颖, 俞小牧, 付北德, 刘阳, 王潇. 基于mtDNA Cyt b基因全序列探讨岩扇贝与3种扇贝的遗传变异水平及其亲缘关系[J]. 大连海洋大学学报, 2018, 33(2): 190-196.
LIAO De－jie, TONG Jin－gou, CAO Shan－mao, ZHOU Ying, YU Xiao－mu, FU Bei－de, LIU Yang, WANG Xiao. Genetic variability and relationship between rock scallop Crassadoma gigantea and three other scallops based
on sequence of mtDNA Cyt b gene. Journal of Dalian Ocean University, 2018, 33(2): 190-196.
2018, Vol. 33 
