Genetic diversity analysis of wild populations of Manila clam Ruditapes philippinarum from China, Japan and Democratic People’s Republic of Korea based on mitochondrial 16S rRNA gene sequence
TAN Yue1，QIU Ming1，LI Jinlong1，ZUO Luya1，WU Wanting1，HUO Zhongming1,2*，YAN Xiwu1,2
1.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2.Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian 116023, China
Abstract: The genetic structure and genetic diversity were compared and analyzed in wild populations of Manila clam Ruditapesphilippinarum collected from Laizhou(LZ), Beihai(BH), Yingkou(YK), and Tianjin(TJ), China, from Hokkaido, Japan(JH) and Sinuiju(NKS), Democratic People’s Republic of Korea(D.P.R.Korea), by amplification and sequencing of mitochondrial 16S rRNA gene fragment to investigate the genetic diversity and genetic differentiation in wild Manila clam in coastal areas of China, Japan and D.P.R.Korea. The results showed that the Manila clam had 16S rRNA sequence amplification fragment of 535 bp, with the contents of 33.3% in T, 10.9% in C, 33.8% in A, 22.0% in G, and A+T of 67.1%. A total of 20 haplotypes were identified from 60 individuals, haplotype sharing among different individuals. There were haploid type diversity (Hd) of 0.378-0.911, nucleotide diversity (Pi) of 0.000 75-0.010 60, and nucleotide differences (K) number of 0.400-5.578 in the six wild populations of Manila clam, with relatively rich genetic diversity in the four populations of Manila clam in Tianjin, Yingkou, Laizhou and Beihai, and with relatively low genetic diversity in the populations of Sinuiju and Hokkaido (Hd<0.5, Pi<0.005), indicating that the six groups of Manila clam were divided into north and south lineages, that is, the North Sea group was the south lineages, and the other five groups were the north lineages. The gene flow of distance-isolated pattern was found among the three populations in northern China, Japan and D.P.R.Korea. In China, the transplanting of Manila clam has a certain influence on the genetic resources of local wild populations. This finding provides reference with the conservation and sustainable utilization of the germplasm resources of Manila clam.
谭月, 邱明, 李金龙, 左陆雅, 吴婉婷, 霍忠明, 闫喜武, . 基于线粒体16S rRNA基因序列的中日朝菲律宾蛤仔野生群体遗传多样性分析[J]. 大连海洋大学学报, 2021, 36(1): 127-134.
TAN Yue, QIU Ming, LI Jinlong, ZUO Luya, WU Wanting, HUO Zhongming, YAN Xiwu, . Genetic diversity analysis of wild populations of Manila clam Ruditapes philippinarum from China, Japan and Democratic People’s Republic of Korea based on mitochondrial 16S rRNA gene sequence. Journal of Dalian Ocean University, 2021, 36(1): 127-134.
2021, Vol. 36 
