Abstract: A feeding trial was conducted to investigate growth, immunity and Cu concentration in some organs of white leg shrimp Litopenaeus vannamei with initial body weight of 0.2 g fed the diets containing 10, 30, 50 mg/kg Cu either as CuSO4 (Cu) or Cu - methionine (MCu). The shrimps were randomly stocked into tanks of each 0.3 m^3 at a rate of 30 individuals per tank, fed 4 times a day at a feeding rate of 8.0% - 10% for 8 weeks and weighted at week 4 and week 8. The serum, hepatopancreas and muscle were sampled and serum phenoloxidase (PO), superoxide dismutase (SOD) and Cu content in hepatopancreas and muscles determined. The results showed that there was no significant difference in weight gain rate (WGR) at the end of 4 weeks in Cu groups. In the MCu groups, however, significantly higher WGR (P 〈0.05) was observed, especially in the shrimp fed the diets containing 10 mg/kg MCu ( MCu - 10 group). The very significantly difference in WGR was found in the two Cu sources at supplemental level of 10 mg/kg diet(P 〈 0.01 ). There was no significant difference in WGR among MCu - 10, MCu - 30 and MCu - 50. But significant differences were found between two Cu sources ( P 〈 0.05) at 30 mg/kg diet at the end of 8 weeks. Supplemental levels and Cu sources did not affected significantly the survival rates at the 4 th and 8^th weeks ( P 〉 0.05). There were significantly higher PO and SOD activities in MCu - 10, MCu-30 and MCu-50 groups than those in the Cu groups (P 〈0.01), no significant difference among MCu - 10, MCu- 30 and MCu -50 groups. The significantly differences in SOD were found between two Cu sources at supplemental level of 30 mg/kg diet ( P 〈 0.05). Cu contents in muscles were not affected by supplemental levels and Cu sources (P 〉 0.05). Cu concentration in hepatopancreas, however, was much significantly higher in MCu - 10, MCu - 30 and MCu - 50 groups than those in the Cu groups. The shrimp fed the diets containing 50 mg/kg Cu as Cu - Methionine had
董晓慧, 杨原志, 郑石轩, 张其华. 饲料中不同铜源和水平对凡纳滨对虾生长、免疫和组织铜含量的影响[J]. 大连海洋大学学报, 2007, 22(5): 377-383.
DONG Xiao-hui, YANG Yuan-zhi, ZHENG Shi-xuan, ZHANG Qi-hua. Effects of dietary copper sources and levels on growth, immunity and Cu concentration in tissues of white leg shrimp Litopenaeus vannamei. Journal of Dalian Ocean University, 2007, 22(5): 377-383.