Effects of dietary vitamin C on hemolymph immune function in marron Cherax quadricarinatus challenged with white spot syndrome virus (WSSV)
ZUO Di, LIU Xiaojie, WU Donglei, LIU Zhiquan, ZHANG Yunfei, MA Chang’an
1.School of Health and Social Care, Shanghai Urban Construction Vocational College, Shanghai 201415, China; 2.Institute of Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 3.School of Life Science, East China Normal University, Shanghai 200062, China; 4.Shanghai Natural History Museum, Natural History Research Center, Shanghai Science & Technology Museum, Shanghai 200041, China
Abstract: To investigate effect of vitamin C (VC) on haemolymph immune function of marron Cheraxquadricarinatus infected with white spot syndrome virus (WSSV)by injection of WSSV, marron with initial body mass of (20.31±2.09)g and initial body length of (19.88±3.17)cm were reared in 18 tanks of each 1.0 m×0.5 m×0.5 m at bottom with 4-6 tiles and 10 pieces of PVC tube at stocking rate of 8 individuals and fed diets containing VC at a dose of 0(control), 100, 200, 400, 800, 1 600 and 3 200 mg/kg at water temperature of 26 ℃ for 8 weeks. The total hemocyte count, phagocytic activity, and hemolytic activity, hemagglutination activity and bacteriostatic rate were detected in the haemolymph in the marron administrated with WSSV infection in 2, 4, 6, and 8 weeks and C-lectin gene expression were analyzed in hepatopancreas, gill, muscle, stomach, intestine, brain, and antennal gland using molecular biology technology in the marron administrated with WSSV infection at a dose of 0, 400, and 800 mg/kg in 2, 4, 6, and 8 weeks. The results showed that the number of hemocytes was increased by 42.26% in the marron in the 800 mg/kg VC supplement group compared with the control group (P<0.05), with the maximal agglutination activity and antibacterial rate in hemolymph of the marron fed the diet containing 800 mg/kg VC, significantly higher than those in the control group (P<0.05). Although 96 hours of WSSV infection significantly led to decrease in the total number of hemocytes, phagocytic activity, agglutination activity and antibacterial rate were significantly higher in 800 mg/kg VC addition group at 96 h than those in control group (P<0.05). There was significantly higher expression of C-lectin gene in hemocytes, the maximal expression level of C-lectin gene was observed in hemocytes, than that in other tissues (P<0.05), with significant decrease in C-lectin gene expression in the marron exposed to WSSV infection (P<0.05), and significantly higher gene expression in 800 mg/kg VC addition group than that in control group and in 400 mg/kg VC addition group (P<0.05). The findings indicated that VC enhanced the immune function of marron fed the diet containing 800 mg/kg of witamin C, showing an immune protective effect on marron against WSSV infection.
左迪, 刘小杰, 吴东蕾, 刘志权, 张云飞, 马长安. 维生素C对白斑综合征病毒(WSSV)侵染后红螯光壳螯虾血淋巴免疫机能的影响[J]. 大连海洋大学学报, 2021, 36(6): 943-951.
ZUO Di, LIU Xiaojie, WU Donglei, LIU Zhiquan, ZHANG Yunfei, MA Chang’an. Effects of dietary vitamin C on hemolymph immune function in marron Cherax quadricarinatus challenged with white spot syndrome virus (WSSV). Journal of Dalian Ocean University, 2021, 36(6): 943-951.
2021, Vol. 36 
