Effects of dietary supplementation of probiotcs on growth，digestive enzyme activity and nutrient profile of body wall in juvenile sea cucumber Apostichopus japonicus
BAO Peng-yun， LI Lu-yao， CHEN Wei， SUN Jing-xian， ZUO Ran-tao， MA Yue-xin
Key Laboratory of Mariculture and Stock Enhancement in North China's Sea， Ministry of Agriculture， Dalian Ocean University， Dalian 116023， China
Abstract: A 8-week feeding trial was conducted to evaluate the effects of probiotics on intestinal bacterial counts，growth，digestive enzyme activity and nutrient profile of body wall in juvenile sea cucumber Apostichopus japonicus.The juvenile sea cucumber with body weight of(0.54±0.06)g were fed basal diet(control group)or a diet supplemented with probiotics containing Rhodotorula sp.H26(1×105cells/g)， Metschnikowia sp.C14(1×105cells/g)and Bacillus sp.BC26(1×107cells/g)at water temperature of 16-24℃.It was found that there were significantly higher intestinal numbers of heterotrophic bacteria in probiotics group than those in the control group(P＜0.05)at week 4 and week 8.The specific growth rate and intestinal pepsin， trypsin，amylase and lipase activities of sea cucumber fed the diet containing probiotics were significantly increased compared to the control group(P＜0.05)at week 4 and week 8.Sea cucumber in probiotics group had higher crude protein and carbohydrate contents in body wall than those in the control group(P＜0.05)at week 4 and 8.The numbers of vibrios， however， were found to be significantly lower in the intestine of sea cucumber fed the diet supplemented with probiotics than those in animals fed the basal diet at week 4 and 8.Sea cucumber in probiotics group had lower ash content in body wall compared with the control group(P＜0.05)at week 8.The findings indicate that a diet supplemented with probiotics leads to improvement of growth and digestive enzyme activity，and nutrient profile of body wall in juvenile sea cucumber.
包鹏云, 李璐瑶, 陈炜, 孙静娴, 左然涛, 马悦欣. 饲料中添加混合益生菌对幼参生长、消化酶活力和体壁营养组成的影响[J]. 大连海洋大学学报, 2018, 33(1): 52-56.
BAO Peng-yun, LI Lu-yao, CHEN Wei, SUN Jing-xian, ZUO Ran-tao, MA Yue-xin. Effects of dietary supplementation of probiotcs on growth，digestive enzyme activity and nutrient profile of body wall in juvenile sea cucumber Apostichopus japonicus. Journal of Dalian Ocean University, 2018, 33(1): 52-56.
2018, Vol. 33 
