Identification and bioinformatics of pathogen Myxobolus honghuensis found in silver crucian carp Carassius auratus gibelio in Qagan Lake
WANG Zhao1,WANG Hao1,QIAN Ai-dong1,ZHOU Jing-xiang1,MA Yue1, ZU Xiu-jie2,LIGai-juan2,LIU Hui-ji2,LIU Yan-hui2
1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2.Jilin Academy of Fishery Sciences,Changchun 130033,China
Abstract: Morphology of diseased silver crucian carp Carassius auratus gibelio collected from a breeding base in Qagan Lake in northeast China was observed and morphometrics of a parasite weremeasured in the diseased fish. The parasite was identified by cloning,sequencing and bioinformatics of 18S rDNA.The results showed that the diseased fish had the typical symptoms ofmyxobolus infection,specially similar to Myxobolus honghuensis in shape and dimensions.Biological information analysis revealed that there was 99.3%similarity of the 18S rDNA sequence between this parasite and Myxobolus honghuensis(GenBank:JF340216).Phylogenetic tree indicated that this parasite and Myxobolushonghuensis were clustered into the same evolutionary branch,confirming that the infection was caused by Myxobolus honghuensis.The findingswere the first report an early infection of Myxobolus honghuensis in northeast China and provided referenceswith the prevention and treatment ofmyxobolus.
王钊, 王好, 钱爱东, 周井祥, 马悦, 祖岫杰, 李改娟, 刘慧吉, 刘艳辉. 一例查干湖异育银鲫洪湖碘泡虫病原的鉴定与生物信息学分析[J]. 大连海洋大学学报, 2015, 30(5): 509-513.
WANG Zhao, WANG Hao, QIAN Ai-dong, ZHOU Jing-xiang, MA Yue, ZU Xiu-jie, LIGai-juan, LIU Hui-ji, LIU Yan-hui. Identification and bioinformatics of pathogen Myxobolus honghuensis found in silver crucian carp Carassius auratus gibelio in Qagan Lake. Journal of Dalian Ocean University, 2015, 30(5): 509-513.
2015, Vol. 30 
