Isolation of bacterial pathogen and histopathological change in cultured turbot with breaking out furunculosis
LIU Jiannan, GUO Yi, NI Ping, GUO Sicong, MA Hongli, LU Xiaocen, YE Shigen*
Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs， Dalian Key Laboratory of Marine Animal Disease Prevention and Control，Dalian Ocean University，Dalian 116023，China
Abstract: A strain of bacterial pathogen named as DLPJC01 was isolated from the diseased turbot Scophthalmusmaximus with body weight of (180±10)g and identified as Aeromonassalmonicida subsp.achromogenes by physicochemical characteristics and PCR amplification of 16S rDNA gene. The pathogenicity of DLPJC01 to turbot was confirmed by challenge experiments. Susceptibility test results showed that this strain was highly sensitive to 5 antibiotics including gentamicin and ceftriaxone, and completely resistant to 13 antibiotics including trimethoprim, ciprofloxacin and kanamycin. Histopathological change indicated that typical acute systemic infection as observed in the diseased turbot, including the clump bacteria in all tissues checked, neutrophilic exudation in muscle tissue with necrosis of myocytes and adipocytes, acute suppurative myositis, and multifocal or diffuse necrosis with nuclear fragmentation and chromatin edging in the liver, spleen, kidney, gills and intestines as well. The findings indicated that liver, spleen, kidney, gill, intestine and muscle in the turbot were the main target organs or tissues of Aeromonassalmonicida, as a typical systemic infection.
刘建男, 郭羿, 倪萍, 郭思聪, 马红丽, 陆晓岑, 叶仕根. 养殖大菱鲆暴发性疖疮病的病原分离与组织病理研究[J]. 大连海洋大学学报, 2020, 35(5): 701-706.
LIU Jiannan, GUO Yi, NI Ping, GUO Sicong, MA Hongli, LU Xiaocen, YE Shigen. Isolation of bacterial pathogen and histopathological change in cultured turbot with breaking out furunculosis. Journal of Dalian Ocean University, 2020, 35(5): 701-706.
2020, Vol. 35 
