Co-production and functional property of polysaccharides from southern oyster Crassostrea rivularis by fractional ultrafiltration
YANG Daqiao 1,2 , WANG Jinxu3, LI Laihao 1* ,YANG Xianqing1, MA Haixia1, HU Xiao1
1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science; 2. College of Food Science and Technology, Shanghai Ocean University; 3. School of Life Sciences and Food Technology, Hanshan Normal University
Abstract: The hydrolysate was separated into three groups including 0-8000(CRRS-A), 8000-200 000(CRRS-B), and >200 000(CRRS-C) from southern oyster Crassostrea rivularis by enzymatic hydrolysis combined with fractional ultrafiltration and the content, molecular weight (MW) distributions, physical and chemical properties, antioxidant activities and basic structure of each group were determined by colorimetric method, high performance liquid chromatography (HPLC),UPLC-Q-Tof-MS/MS, and Fourier infrared spectrum(FTIR). The results showed that the CRRS-A was primarily comprised of polypeptide (87.26±1.65)%, with MW of less than 2000 and containing 10 compounds. The maximal antioxidant activities including 2,2-diphenyl-1-picrylhydrazide (DPPH) free radical scavenging ability and hydroxyl radical scavenging ability were observed in CRRS-A, and the superoxide radical scavenging ability was significantly higher in CRRS-A than that in antioxidant glutathione (GSH) (116.67±3.22)U/g. The CRRS-B was found to contain polysaccharide (75.82±0.28)%, including glucuronic acid (5.06±0.60)%, methyl pentose (31.23±1.29)%, sulfate radical (23.47±0.7)%, and glucosamine hydrochloride(2.29±0.04)%, with MW of less than 8300-150 000, assuming pyranose carbohydrate with high antioxidant activity varying between 2,6-di-tert-butyl-4-4-methylphenol(BHT) and GSH. The CRRS-C was pyranotype glycoprotein with a molecular weight of 6 600-7 152 000 and had poor antioxidant activity in vitro. The findings indicated that fractional ultrafiltration system separated polypeptide and polysaccharide from southern oyster effectively and that the membrane separation components of enzymatic hydrolysates from southern oyster CRRS-A showed high antioxidant activity in vitro, CRRS-B showing better antioxidant activity than for BHT.
杨大俏, 王锦旭, 李来好, 杨贤庆, 马海霞, 胡晓. 近江牡蛎膜分离联产制备多糖多肽及其功能特性研究[J]. 大连海洋大学学报, 2020, 35(1): 126-133.
YANG Daqiao, WANG Jinxu, LI Laihao, YANG Xianqing, MA Haixia, HU Xiao. Co-production and functional property of polysaccharides from southern oyster Crassostrea rivularis by fractional ultrafiltration. Journal of Dalian Ocean University, 2020, 35(1): 126-133.
2020, Vol. 35 
