一株鲤源降血糖赖氨酸芽孢杆菌的筛选鉴定及其培养基优化

doi:10.16535/j.cnki.dlhyxb.2021-281

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大连海洋大学学报 ›› 2022, Vol. 37 ›› Issue (4) : 611-619. DOI: 10.16535/j.cnki.dlhyxb.2021-281

一株鲤源降血糖赖氨酸芽孢杆菌的筛选鉴定及其培养基优化

一株鲤源降血糖赖氨酸芽孢杆菌的筛选鉴定及其培养基优化

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Screening, identification and culture medium optimization of a hypoglycemic Lysinibacillus fusiformis strain from common carp

YU Junfei, XIA Banghua*, HAO Qirui, ZOU Haobo, WANG Peng, CHEN Zhongxiang,GAO Lei, WU Song, HUANG Li, QIN Dongli*, HAN Ying*

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摘要

为研究赖氨酸芽孢杆菌Lysinibacillus fusiformis的降血糖功能并提升其代谢产物浓度，通过产蛋白酶能力试验,从鲤肠道内含物中筛选具有蛋白分解能力的细菌，并对其产二肽基肽酶-Ⅳ(DDP-Ⅳ)抑制剂的能力进行测定，筛选出的菌株经分离纯化培养后,通过16S rRNA测序和生理生化特征进行鉴定，并通过单因素试验和响应面优化试验,对菌株的培养基配方进行配比优化。结果表明：筛选到一株产DDP-Ⅳ抑制剂能力较强的益生菌CR-3(37 ℃时产率为62.35%)，通过生理生化试验和构建系统进化树，将CR-3菌株鉴定为赖氨酸芽孢杆菌；响应面优化试验显示，CR-3菌株的最佳发酵培养基配方为蔗糖1.46%、CaCO3 0.55%、NH4Cl 0.48%及豆粕2.10%(均为质量分数)，在此条件下，发酵培养基的OD600 nm值较初始发酵培养基的OD600 nm值提升了64%。研究表明，本试验中筛选出的赖氨酸芽孢杆菌具有良好的产DDP-Ⅳ抑制剂能力，并具有潜在的降鲤血糖功能。

Abstract

In order to investigate the hypoglycemic function of bacillus Lysinibacillus fusiformis and thus enhance its metabolite concentration, bacteria with proteolytic ability were screened from common carp Cyprinus carpio intestinal contents by protease production ability assay, and their ability to produce dipeptidyl peptidase-Ⅳ (DDP-Ⅳ) inhibitor was measured. The screened strains were isolated, purified and cultured, then identified by 16S rRNA sequencing and physiological and biochemical characteristics, and the medium formulation of the strains was by single-factor test and response surface optimization experiment. The results showed that the CR-3 strain, with the ability to produce 62.35% at 37 ℃, was identified as L.fusiformis by physiological and biochemical identification and phylogenetic tree construction. The optimum fermentation medium was consisted of 1.46% sucrose, 0.55% CaCO3, 0.48% NH4Cl and 2.10% soybean meal, with 64% higher DDP-Ⅳ inhibitor yield than the strain unoptimized. The findings indicate that the L.fusiformis screened in this experiment has good ability to produce DDP-IV inhibitor and has potential hypoglycemic function.

导出引用

于珺妃, 夏邦华, 郝其睿, 邹昊博, 王鹏, 陈中祥, 高磊, 吴松, 黄丽, 覃东立, 韩英. 一株鲤源降血糖赖氨酸芽孢杆菌的筛选鉴定及其培养基优化[J]. 大连海洋大学学报, 2022, 37(4): 611-619 https://doi.org/10.16535/j.cnki.dlhyxb.2021-281

YU Junfei, XIA Banghua, HAO Qirui, ZOU Haobo, WANG Peng, CHEN Zhongxiang, GAO Lei, WU Song, HUANG Li, QIN Dongli, HAN Ying. Screening, identification and culture medium optimization of a hypoglycemic Lysinibacillus fusiformis strain from common carp[J]. Journal of Dalian Fisheries University, 2022, 37(4): 611-619 https://doi.org/10.16535/j.cnki.dlhyxb.2021-281

中图分类号： S 948