Genetic analysis of cultured catfish Silurus asotus using TRAP technique
GE Xue-liang, SUN Zhong-wu, YIN Hong-bin
1. College of Life Science, Northeast Forestry Univ. , Harbin 150040, China; 2. Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
Abstract: TRAP (Target Region Amplified Polymorphism) was first used for research of genetic diversity in catfish Silurus asotus. Nine pairs of primers selected from 24 primers produced good amplified patterns with 37.5% of polymorphic primer was in all amplified primers. There were 15 loci amplified by one primer, and 75 polymorphic loci were obtained in all 135 amplified loci with 55.56% of polymorphic loci. The genetic variation analyses showed that TRAP marker can be used in genetic structure research of the catfish. The higher percentage of polymorphic primer number indicated that TRAP as a DNA molecular marker can be used in genetic linkage map research for Silurus asotus.