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大连海洋大学学报  2015, Vol. 30 Issue (4): 374-379    DOI: 10.16535/j.cnki.dlhyxb.2015.04.006
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利用Bayesian方法进行牙鲆体质量和主要形态性状的QTL定位及互作分析
崔妍1,高会晓1,仇雪梅1、2,杨润清3,刘海金3
1.大连海洋大学水产与生命学院,辽宁大连116023;2.大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023; 3.中国水产科学研究院,北京100141
Bayesian mapping QTL for body weight and morphological traits in Japanese flounder Paralichthys olivaceus
CUIYan1,GAO Hui-xiao1,QIU Xue-mei1,2,YANG Run-qing3,LIU Hai-jin3
1.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;2.Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China;3.Chinese Academy of Fishery Sciences,Beijing 100141,China
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摘要 为了定位牙鲆Paralichthysolivaceus体质量和形态性状的QTL,利用雌核发育方法获得双单倍体牙鲆群体的157个个体,同时构建了包含481个SSR标记和141个EST-SSR标记的遗传连锁图谱,并采用Bayesian模型选择方法对研究性状进行主效应QTL定位和上位性互作分析。结果表明:共检测到38个主效应QTL和50对上位互作效应,其中,大部分主效应QTL位于5、6、9、22号染色体,上位互作效应所解释的表型方差为2.3%~23.1%;8个主效应QTL和7对互作QTL表现出多效性,均分别对两个以上的性状起作用。本研究结果将有助于提高牙鲆育种中对有益经济性状的选择效率。
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崔妍
高会晓
仇雪梅
杨润清
刘海金
关键词:  Bayesian选择模型  牙鲆DH群体  互作分析  R/qtlbim  遗传连锁图谱    
Abstract: A doubled haploids(DH)population of 157 individualswas established in Japanese flounder Paralich+ thys olivaceus by mitotic gynogenetics,and genetic linkage map including 481 type II SSRs and 141 EST-SSRs were constructed in order tomap QTL for the body weight and morphological traits.Subsequently,themain effect QTL and epistatic interactions for the observed traitswere analyzed by Bayesian model selection.Itwas found that 38 main-effect QTL located on the chromosomes 5,6,9,and 22 with the proportion of phenotypic variance explained by interacting QTLs ranging from 2.3%to 23.1%,and 50 pairs of epistatic interactionswere detected for themeasured traits.There were 8 pleiotropymain effect QTL's and 7 pairs of epistatic interactions,both governing simultaneously more than two traits,respectively,in themain effect QTLs.The findings improve the selection efficiency for the interest traits in Japanese flounder breeding.
Key words:  Bayesian model selection    DH population of Paralichthys olivaceus    analysis of interaction    R/qtlbim    genetic linkagemap
                    发布日期:  2016-12-30      期的出版日期:  2015-08-21
中图分类号:  S917.4  
引用本文:    
崔妍, 高会晓, 仇雪梅, 杨润清, 刘海金. 利用Bayesian方法进行牙鲆体质量和主要形态性状的QTL定位及互作分析[J]. 大连海洋大学学报, 2015, 30(4): 374-379.
CUIYan, GAO Hui-xiao, QIU Xue-mei, YANG Run-qing, LIU Hai-jin. Bayesian mapping QTL for body weight and morphological traits in Japanese flounder Paralichthys olivaceus. Journal of Dalian Ocean University, 2015, 30(4): 374-379.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.16535/j.cnki.dlhyxb.2015.04.006  或          https://xuebao.dlou.edu.cn/CN/Y2015/V30/I4/374
[1] 卢建峰, 白俊杰, 李胜杰, 樊佳佳, 徐浩. 大口黑鲈遗传连锁图谱的构建[J]. 大连海洋大学学报, 2011, 26(2): 138-143.
[2] 葛学亮, 孙中武, 尹洪滨. 人工养殖鲶的TRAP遗传分析[J]. 大连海洋大学学报, 2010, 25(1): 76-79.
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