Abstract: Amplified fragment length polymorphism （AFLP） was used to detect the genetic variation in two populations of roughskin sculpin Trachidermus fasciatus collected from Dandong （Liaoning province） and Qinhuangdao （ Hebei province）. It was found that 214 polymorphic markers were produced out of the total of 360 bands amplified from 63 individuals by six EcoRI and MseI primer combinations. The proportions of polymorphic loci were 50.28% for Dandong population and 50.00% for Qinhuangdao population and the average heterozygosity and Shannon＇s Information index were 0. 1690 and 0. 1733 for Dandong population, and 0.2534 and 0.2592 for Qinhuangdao population, indicating that the genetic diversity index of the two populations showed the same level. Shannon＇s Information index and AMOVA analysis revealed that the genetic variation was mainly derived from individuals within populations and there was no significant genetic divergence between the populations. The UPGMA tree based on AFLP data supported this result and no distinct lade was detected. Dominant gene frequency revealed that there was the same genetic population structure in the two populations.
2008, Vol. 23 
