Identification and Application of Microsatellite Makers from BAC and ESTs Sequence in redfin puffer （ Takifugu rubripes）
HAO Jun, SUN Xiao-wen, MENG Xue-song, FENG Jian
1. School of Life Science and Technology, Dalian Fisheries Univ. , Dalian 116023, China; 2. Key Lab. of Bioengineering Breeding of Northern Finfish, Ministry of Agriculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 3. Dalian Tianzheng Co. , Ltd. , Dalian 1160021,China
Abstract: Microsatellites as a new kind of molecular marker are extremely important markers, and have more advantages over the traditional genomic- derived simple sequence repeats （SSRs）. Twenty- two new microsateUites were identified from redfin puffer （ Takifugu rubripes） BAC and EST library found in UK MRC HGMP - RC （ the Fugu Genomics Project） by using bioinformatics software of Tandem Repeats Finder. Twenty - two pairs of micrsat- ellite primer were designed and applied to investigate the polymorphism in the populations of redfin puffer from Zhuanghe, Hebei I and Hebei II. All pairs of primer generated PCR amplification products, and 9 Out of 11 （ expressed sequence tags） EST - SSRs primers yielded products of expected size while 20 pairs of primer containing polymorphisms. There was lower allele number in the microsateUites located in ESTs than those isolated from a BAC library. These novel microsatellites will be very useful for population genetic and reproductive strategies studies, as well as for screening economic character of redfin puffer.
2007, Vol. 22 
