The electrophoresis of isoenzymes in three breeds of sea mustard Undaria pinnatifuta
YANG Hong-yi, ZHAO Wen, LI Yu-wei, ZHANG Peng, YANG Dan-guang, ZHANG Guo-hui, TAN Xue-en, CHEN Gang, WANG Shen
1. Test and Quarantine Bureau in Dayaowan, Dalian 116601, China ;2. Key Laboratory of Hydrobiology., College of Life Science and Biotechnology, Dalian Fisheries University, Dalian 116023, China ;3. Laohutan Marine Park of Dalian, Dalian 116000, China; 4. Test and Quarantine Bureau in Liaoning Prouince,Dalian 1116001,China
Abstract: Peroxidase (POD)and esterase (EST) isoenzymes were analysed in sporophyll, stem and leaf in three populations ( native, Japanese and half - bred ) of sea mustard Undaria pinnatifida by electrophoresis. The results showed that the POD activity was higher in the sporophyll than that in the stems and leaves in which the POD activities were weak from bottom to top in native plant. For the Japanese plant, the POD activities of the stems and leaves were the same in all positions. However, the activity was slightly higher in midportion of a leaf than that in stems. The high enzyme activities were observed in mid - bottom stems in the haftbred plant, and the activity was higher in bottom leaf than that in mid -top leaf. The EST enzyme activity was higher in the sporophyll than that in the stems and leaves. The methods of sample treatment, and relationship among the breeds were discussed.
杨弘诣, 赵文, 李裕伟, 张鹏, 杨旦光, 张国辉, 谭学恩, 程刚, 王深. 3个不同养殖群体裙带菜同工酶的电泳分析[J]. 大连海洋大学学报, 2006, 21(1): 59-63.
YANG Hong-yi, ZHAO Wen, LI Yu-wei, ZHANG Peng, YANG Dan-guang, ZHANG Guo-hui, TAN Xue-en, CHEN Gang, WANG Shen . The electrophoresis of isoenzymes in three breeds of sea mustard Undaria pinnatifuta. Journal of Dalian Ocean University, 2006, 21(1): 59-63.